Fermentation characteristics in stirred-tank reactor of exopolysaccharides with hypolipidemic activity produced by <i>Pleurotus geesteranus</i>5<sup>#</sup>

DUOBIN, MAO; YUPING, MA; LUJING, GENG; AIJING, ZHAO; JIANQIANG, ZHENG; CHUNPING, XU

doi:10.1590/0001-3765201320130037

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Biological Sciences • An. Acad. Bras. Ciênc. 85 (4) • 2013 • https://doi.org/10.1590/0001-3765201320130037 copy

Fermentation characteristics in stirred-tank reactor of exopolysaccharides with hypolipidemic activity produced by Pleurotus geesteranus5^#

Authorship SCIMAGO INSTITUTIONS RANKINGS

In this study, the hypolipidemic effect of exopolysaccharides (EPS) fromPleurotus geesteranus 5^# fermenting liquor by the optimal culture conditions in a 5-L stirred-tank reactor was investigated. The hypolipidemic effect of the polysaccharide, investigated in streptozotocin induced diabetic mice, decreased plasma glucose, total cholesterol and triacylglycerol concentrations by 17.1 %, 18.8 % and 12.0 %, respectively. The results of the present investigation strongly demonstrate the potential of this polysaccharide to prevent hyperglycemia in the experimental animals. Under optimal culture conditions, the maximum concentrations of mycelial and EPS were 22.63 g/L after 7 d cultivation and 11.09 g/L after 10 d, respectively. Furthermore, the morphological parameters (i.e. mean diameter, circularity, roughness and compactness) of the pellets and the broth viscosity were characterized. It was proved that compactness of the pellet morphology (R²=0.963, p<0.01) was significantly and positively determined with mycelial biomass. Moreover, mean diameter (R²=93.3, p<0.01) and broth viscosity (R²=0.950, p<0.01) were significantly and positively determined with EPS content.

exopolysaccharides; Pleurotus geesteranus ; fermentation; hypolipidemic effect; morphology

Group	Oral administration
Normal^a a Group of 8 normal mice.	None
Control^b b Diabetic mice induced by streptozotocin (50mg/kg body weight) for 8 mice.	0.9% NaCl
EPS^b b Diabetic mice induced by streptozotocin (50mg/kg body weight) for 8 mice.	200 mg/kg•d P. geesteranus 5^# EPS

Group¹ 1 See Table I. Each value is the mean ± SE (n = 8).	Liver (g/100g BW)	Kidney (g/100g BW)	Spleen (g/100g BW)	Pancreas (g/100g BW)
Normal	4.16 ± 0.05^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	1.31 ± 0.15^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	0.30 ± 0.08^NS	0.35 ± 0.07^NS
Control	5.34 ± 0.08^c a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	1.75 ± 0.11^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	0.29 ± 0.04	0.31 ± 0.09
EPS	4.79 ± 0.09^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	1.54 ± 0.10^ab a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	0.28 ± 0.07	0.42 ± 0.05

Group¹ 1 See Table I.	Normal	Control	EPS
plasma glucose, total cholesterol, and triglyceride levels
Glucose (mmol/L)	5.97±1.17^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	36.90±0.99^c a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	30.58±2.80^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.
Total cholesterol (mmol/L)	2.82±0.26^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	4.64±0.13^c a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	3.77±0.40^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.
Triglyceride (mmol/L)	1.23±0.10^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	1.67±0.06^c a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	1.47±0.06^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.
plasma HDL cholesterol, LDL cholesterol, and atherogenic index
HDL cholesterol (mmol/L)	1.36±0.06^c a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	0.77±0.08^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	1.04±0.10^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.
LDL cholesterol² 2 Total cholesterol - HDL cholesterol - (Triglyceride/5). (mmol/L)	1.39±0.07^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	3.54±0.04^c a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	2.60±0.29^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.
Atherogetic Index³ 3 (Total cholesterol - HDL cholesterol)/ HDL cholesterol. Each value is mean ± SE for 8 mice.	1.20±0.10^a a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	5.06±0.50^c a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.	2.78±0.07^b a,b,c Values with different superscript letters in the same column significantly different among the groups at p<0.05.

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[1] Chun-Ping Xu E-mail: c.p.xu@zzuli.edu.cn

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Fermentation characteristics in stirred-tank reactor of exopolysaccharides with hypolipidemic activity produced by Pleurotus geesteranus5#

Fermentation characteristics in stirred-tank reactor of exopolysaccharides with hypolipidemic activity produced by Pleurotus geesteranus5^#