Atomic force microscopy reveals different surface morphologies of functionally-different glial cells

SESSIONS OF THE ACADEMIA BRASILEIRA DE CIÊNCIAS

SUMMARY OF COMMUNICATIONS

THEMES ON NEUROSCIENCE

ATOMIC FORCE MICROSCOPY REVEALS DIFFERENT SURFACE MORPHOLOGIES OF FUNCTIONALLY-DIFFERENT GLIAL CELLS^*

¹²¹²¹

¹

²Depto. Anatomia, ICB, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, RJ, Brazil.

Astroglial cells are involved in directional movements of neurons such as migration of the neuronal cell body and growth of neurites. In the mammalian midbrain, medial (M) and lateral (L) radial glia and derived astrocytes differ in their ability to support neuritic growth. In previous work, we have demonstrated that the growth-permissive ability of L astrocytes and non-permissive properties of M astrocytes correlate with the respective composition of the cell surface-associated and secreted glycosaminoglycans (GAGs). Recent work also shows that the GAG-degrading enzyme heparitinase I increases the neurite growth-promoting ability of M midbrain astrocytes (Garcia-Abreu J et al. 2000 Glia 29: 260). In agreement with previous AFM studies of living glial cell lines and cells in primary culture, imaging of living L and M cells at similar load forces showed structures identified as F-actin fibers. Moreover, no systematic differences were observed between L and M pictures. By contrast, the surfaces of formaldehyde-fixed lateral (L) and medial (M) astrocytes differ by the presence of conspicuous 250 nm protrusions in the former, and of a fibrillar network in the extracellular matrix of the latter. Furthermore, we show that treatment with heparitinase I leads to disappearance of the fibrils from M cells and, consequently, to the assumption of an L-like appearance. Our results suggest that the formation of fibrils may follow from an ability to form large aggregates by association of HS carbohydrate units as has been unexpectedly detected by AFM for oligomers of polysialic acid or polysialic acid-containing carbohydrate units of N-CAM, with formation of filament bundles (Toikka J et al. 1998. J Biol Chem 273: 28557). Taken together with the functional effects of heparitinase I treatment, our present results demonstrate an important role of the extracellular matrix on the functional properties of astrocytes. They also emphasize the power and potentialities of AFM in the study of the extracellular matrix on the surface of fixed cells. — (June 27, 2000 ).

Publication Dates

• Publication in this collection
  05 Oct 2000
• Date of issue
  Sept 2000