<i>In vitro</i> anthelmintic activity of <i> Psidium guajava</i> hydroalcoholic extract against gastro-intestinal sheep nematodes

SILVA, LUCIANA P.; DEBIAGE, RAFAEL R.; BRONZEL-JÚNIOR, JOÃO L.; SILVA, REGILDO M.G. DA; -PEIXOTO, ERIKA C.T. MELLO

doi:10.1590/0001-3765202020190074

home Sumário navigate_before Anterior Atual Seguinte navigate_next

home Sumário

BIOLOGICAL SCIENCES • An. Acad. Bras. Ciênc. 92 (suppl 2) • 2020 • https://doi.org/10.1590/0001-3765202020190074 linkcopy

In vitro anthelmintic activity of Psidium guajava hydroalcoholic extract against gastro-intestinal sheep nematodes

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Tanniferous plants have been used for ruminants verminosis control and represent a possibility to minimize the pharmacological resistance against conventional antiparasitics. This study aimed to evaluate the antihelminthic activity of the hydroalcoholic extract of stem bark of guava tree (PgHA). It was performed the hatchability and larval migration inhibition assays to evaluate PgHA at the following concentrations 0.62, 1.25, 2.5 and 5.0 mg mL^-1 and the control treatments. The total polyphenol, flavonoid and tannin contents were determined by phytochemical analysis, high performance liquid chromatography coupled to mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl, ferric reducing antioxidant power and thiobarbituric acid reactive substances tests. It was also determinated total protein, intracellular H₂O₂ and antioxidant activity of enzimes: glutathione S-transferase and superoxide dismutase. PgHA was able to inhibit both hatchability and larval migration, but only hatchability inhibition presented dose-dependent pattern. The antioxidant activity was demonstrated by linear regression with IC₅₀ corresponding to 534.02 μg mL^-1. The antiparasitic mechanism occurred through pro-oxidative activity by the increase of total proteins, intracellular H₂O₂ and the lipid peroxidation products, as well as the increase of the enzymes above related. Thus, the PgHA showed antiparasitic activity in vitro.

Key words
Gastrointestinal nematodes; Guava tree; medicinal plants; tannins; parasite

Concentration (µg.mL^-1)	DPPH^a	FRAP	TBARS	Total Phenols^b	Total Flavonoids^c	Total Tannins^d
25	2.26±0.91	64.74±8.43	26.07±3.58	-	71.57	-
50	12.43±0.36	128.07±10.32	22.16±7.10	-	100.29	-
75	9.55±0.63	175.30±23.13	16.57±3.22	29.74	156.76	-
100	18.67±3.26	205.30±43.32	13.59±2.58	45.38	344.29	0.68
250	56.59±8.37	579.55±53.89	16.38±1.29	133.03	382.86	6.27
500	87.49±1.39	986.60±78.89	4.28±0.61	165.49	460.00	74.49
1000	86.81±2.23	1317.89±41.37	4.28±0.61	156.33	767.62	177.94

Treatments mg.m^-1	TP (mg.mL^-1)	PH (µM.g^-1)	LP (mM.L^-1)	GST (nm.mL^-1.min^-1)	SOD U.mL^-1)
0	38.04±2.32^a	0,19±0.02a	0,37±0.09a	1.64±0.23a	0.73±0.06a
0.62	42.31±1.98^a	0.27±0.09b	0,45±0.12a	1.86±0.21a	0.75±0.09a
1.25	53.23±2.33b	0.35±0.02b	0,69±0.18b	2.17±0.14b	0.89±0.24a
2.50	89.54±2.65c	0.61±0.06c	1,18±0.23c	2.69±0.16c	1.23±0.11b
5.00	197.67±3.42d	1.47±0.11d	2,65±0.63d	3.89±0.14d	3.49±0.23c

vertical_align_top show_chart

more_horiz close
- image
- translate
- link
- article
- vertical_align_top
- file_download
- show_chart
- image
- translate
- link
- article

location_on

Academia Brasileira de Ciências Rua Anfilófio de Carvalho, 29, 3º andar, 20030-060 Rio de Janeiro RJ Brasil, Tel: +55 21 3907-8100 - Rio de Janeiro - RJ - Brazil
E-mail: aabc@abc.org.br

rss_feed Acompanhe os números deste periódico no seu leitor de RSS

[tfnI] Values are expressed as mean ±SE. Same letters within the same column indicate no signiﬁcant differences among samples by Tukey test (α≤0.05). ^aPercentage values of DPPH radical scavenging activity; ^bValues of total phenol levels (mg of gallic acid equivalent/g of extract); ^cTotal flavonoid levels in quercetin equivalent per mg/g of extract; ^dTannic acid equivalent per gram of the extract (mg TAE g^-1).