Effects of Tityus stigmurus (Thorell 1876) (Scorpiones: Buthidae) venom in isolated perfused rat kidneys

ABSTRACT

Scorpions belonging to the Tityus genus are of medical interest in Brazil. Among them, Tityus stigmurus is the main scorpion responsible for stings in the Northeast region. After a sting, the scorpion venom distributes rapidly to the organs, reaching the kidneys quickly. However, there are few studies concerning the renal pathophysiology of scorpion poisoning. In this study, we evaluated the effects of T. stigmurus venom (TsV) on renal parameters in isolated rat kidneys. Wistar rats (n = 6), weighing 250-300 g, were perfused with Krebs-Henseleit solution containing 6 g/100 mL bovine serum albumin. TsV at 0.3 and 1.0 μg/mL was tested, and the effects on perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR), and electrolyte excretion were analyzed. Effects were observed only at TsV concentration of 1.0 μg/mL, which increased PP (_controlPP_40' = 92.7 ± 1.95; _TsVPP_40' = 182.0 ± 4.70^* mmHg, ^*p < 0.05), RVR (_controlRVR_40' = 3.28 ± 0.23 mmHg; _TstRVR_40' = 6.76 ± 0.45^* mmHg, ^*p < 0.05), UF (_controlUF_50' = 0.16 ± 0.04; _TstUF_50' = 0.60 ± 0.10^* mL/g/min,^*p < 0.05), GFR and electrolyte excretion, with histological changes that indicate renal tubular injury. In conclusion, T. stigmurus venom induces a transient increase in PP with tubular injury, both of which lead to an augmented electrolyte excretion.

Key words:
2D-PAGE; kidney; MDCK; venom; Tityus stigmurus