Second-generation ethanol production by <i>Wickerhamomyces anomalus</i> strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure

SEHNEM, NICOLE T.; MACHADO, ÂNGELA S.; MATTE, CARLA R.; MORAIS JR, MARCOS ANTONIO DE; AYUB, MARCO ANTÔNIO Z.

doi:10.1590/0001-3765202020181030

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BIOLOGICAL SCIENCES • An. Acad. Bras. Ciênc. 92 (suppl 2) • 2020 • https://doi.org/10.1590/0001-3765202020181030 linkcopy

Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure

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Abstract

The aims of this work were to improve cell tolerance towards high concentrations of furfural and 5-hydroxymethylfurfural (HMF) of an osmotolerant strain of Wickerhamomyces anomalus by means of evolutionary engineering, and to determine its ethanol production under stress conditions. Cells were grown in the presence of furfural, HMF, either isolated or in combination, and under high osmotic pressure conditions. The most toxic condition for the parental strain was the combination of both furans, under which it was unable to grow and to produce ethanol. However, the tolerant adapted strain achieved a yield of ethanol of 0.43 g g^-1glucose in the presence of furfural and HMF, showing an alcohol dehydrogenase activity of 0.68 mU mg protein^-1. For this strain, osmotic pressure, did not affect its growth rate. These results suggest that W. anomalus WA-HF5.5strain shows potential to be used in second-generation ethanol production systems.

Key words
Second-generation ethanol; Wickerhamomyces anomalus; furaldehydes tolerance; osmotic pressure; lignocellulosic hydrolysates

	Strains
Condition	WA-001	WA-HF5.5	WA-001	WA-HF5.5	WA-001	WA-HF5.5	WA-001	WA-HF5.5	WA-001	WA-HF5.5	WA-001	WA-HF5.5
	Biomass (g L^-1)		µ_max (h^-1)		Ethanol (g L^-1)		Y_P / _S (g g^-1)		Q_p(g L^-1 h^-1)		Glycerol (g L^-1)
Control	7.79 ± 0.10	6.93 ± 0.22	0.35	0.30	5.81 ± 0.15	5.82 ± 0.17	0.30	0.35	0.24	0.47	0.40 ± 0.05	0.48 ± 0.05
HMF 3 g L^-1	7.12 ± 0.26	6.29 ± 0.08	0.21	0.19	6.07 ± 0.12	5.87 ± 0.14	0.27	0.30	0.18	0.24	1.98 ± 0.09	1.54 ± 0.02
Furfural 3 g L^-1	0.05 ± 0.00	0.18 ± 0.05	0.00	0.06	0.51 ± 0.03	4.1 ± 0.17	0.00	0.22	0.00	0.08	0.00 ± 0.00	1.56 ± 0.14
Furfuraland HMF	0.00 ± 0.00	3.41 ± 0.12	0.00	0.19	0.00 ± 0.00	4.7 ± 0.31	0.00	0.43	0.00	0.16	0.00 ± 0.00	1.81 ± 0.11

Condition	Osmolarity (mM kg^-1)	Specific Growth Rate μ (h^-1)
		WA-001	WA-HF5.5
YPD	209	0.31	0.28
YPD + 0.25 M NaCl	661	0.29	0.29
YPD + 0.50 M NaCl	1024	0.27	0.27
YPD + 0.75 M NaCl	1350	0.24	0.28
YPD + 1.00 M NaCl	1653	0.21	0.25
YPD + 1.25 M NaCl	1969	0.18	0.23
YPD + 1.50 M NaCl	2060	0.08	0.21

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