Scanning electron microscopy of dermatofibroma

Dermatofibroma is a proliferation of spindle cells located in the dermis. We used scanning electron microscopy to examine two histologically confirmed lesions and observed preserved collagen bundles in the perilesional area. In the lesional area, the collagen was denser, without formation of bundles. Higher magnification showed collagen with mesh-like appearance similar to stretched tufts of cotton. Very high magnification evidenced the tufts of cotton and spindle cells measuring 2 to 12 microns.

Keywords:
Collagen; Histiocytoma, Benign Fibrous; Microscopy, Electron, Scanning

Authorship

Felipe Simões Lopes Quintana

Post-Graduation Program in Health and Behavior, Universidade Católica de Pelotas, Pelotas (RS), Brazil.Universidade Católica de PelotasBrazilPelotas, RS, BrazilPost-Graduation Program in Health and Behavior, Universidade Católica de Pelotas, Pelotas (RS), Brazil.

University Hospital, Universidade Federal de Pelotas, Pelotas (RS), Brazil.Universidade Federal de PelotasBrazilPelotas, RS, BrazilUniversity Hospital, Universidade Federal de Pelotas, Pelotas (RS), Brazil.

https://orcid.org/0000-0003-2781-1869

Hiram Larangeira de Almeida Jr.Mailing Address: Hiram Larangeira de Almeida Jr. E-mail: hiramalmeidajr@hotmail.com

Department of Dermatology, Universidade Federal de Pelotas, Pelotas (RS), Brazil.Universidade Federal de PelotasBrazilPelotas, RS, BrazilDepartment of Dermatology, Universidade Federal de Pelotas, Pelotas (RS), Brazil.

https://orcid.org/0000-0003-0705-1778

Caroline Pires Ruas

Electron Microscopy Center of the South, Fundação Universidade de Rio Grande, Rio Grande (RS), Brazil.Fundação Universidade de Rio GrandeBrazilRio Grande, RS, BrazilElectron Microscopy Center of the South, Fundação Universidade de Rio Grande, Rio Grande (RS), Brazil.

https://orcid.org/0000-0002-6917-1727

Valéria Magalhães Jorge

Pathology Department, Universidade Federal de Pelotas, Pelotas (RS), Brazil.Universidade Federal de PelotasBrazilPelotas, RS, BrazilPathology Department, Universidade Federal de Pelotas, Pelotas (RS), Brazil.

https://orcid.org/0000-0003-3153-6847

Mailing Address: Hiram Larangeira de Almeida Jr. E-mail: hiramalmeidajr@hotmail.com

Conflict of interest: None.

AUTHORS’CONTRIBUTIONS

Felipe Simões Lopes Quintana

0000-0003-2781-1869

Approval of the final version of the manuscript; Conception and planning of the study; Elaboration and writing of the manuscript; Obtaining, analyzing and interpreting the data; Critical review of the literature; Critical review of the manuscript

Hiram Larangeira de Almeida Jr

0000-0003-0705-1778

Approval of the final version of the manuscript; Conception and planning of the study; Elaboration and writing of the manuscript; Obtaining, analyzing and interpreting the data; Effective participation in research orientation; Critical review of the literature; Critical review of the manuscript

Caroline Pires Ruas

0000-0002-6917-1727

Approval of the final version of the manuscript; Obtaining, analyzing and interpreting the data; Critical review of the manuscript

Valéria Magalhães Jorge

0000-0003-3153-6847

Approval of the final version of the manuscript; Conception and planning of the study; Elaboration and writing of the manuscript; Obtaining, analyzing and interpreting the data; Effective participation in research orientation; Intellectual participation in propaedeutic and/or therapeutic conduct of the cases studied; Critical review of the literature; Critical review of the manuscript

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (3)

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Figure 1
Light Microscopy - A - Collagen homogenization with cell proliferation (asterisks) with normal collagen in the lower portion (NC) (hematoxylin & eosin, x150). B - similar aspect in the lesion border, with involvement of the papillary dermis (hematoxylin & eosin, x150). C - detail of the fusiform cells (arrows) (hematoxylin & eosin, x400). D - diminished elastic fibers in the homogenous area, with clear demarcation of normal elastic tissue (arrows) in the lower portion (Weigert’s stain, x150)

Thumbnail

Figure 2
Scanning electron microscopy. A - Low magnification of the perilesional area with normal collagen bundles (x450). B - High er magnification of normal collagen bundles (x1.700). C - Low mag nification of the lesional area with compact tufted collagen (x450). D - Higher magnification of affected compacted collagen (x2.000)

Thumbnail

Figure 3
Scanning electron microscopy. A - Detail of the affected area with tufted collagen without bundle formation (x3.000). B e C - Detail of fusiform cells, measuring 2 to 12 microns (x7,000 and x8,000)

Figure 1 Light Microscopy - A - Collagen homogenization with cell proliferation (asterisks) with normal collagen in the lower portion (NC) (hematoxylin & eosin, x150). B - similar aspect in the lesion border, with involvement of the papillary dermis (hematoxylin & eosin, x150). C - detail of the fusiform cells (arrows) (hematoxylin & eosin, x400). D - diminished elastic fibers in the homogenous area, with clear demarcation of normal elastic tissue (arrows) in the lower portion (Weigert’s stain, x150)

Figure 2 Scanning electron microscopy. A - Low magnification of the perilesional area with normal collagen bundles (x450). B - High er magnification of normal collagen bundles (x1.700). C - Low mag nification of the lesional area with compact tufted collagen (x450). D - Higher magnification of affected compacted collagen (x2.000)

Figure 3 Scanning electron microscopy. A - Detail of the affected area with tufted collagen without bundle formation (x3.000). B e C - Detail of fusiform cells, measuring 2 to 12 microns (x7,000 and x8,000)

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