Blood gas analyzer utility in evaluating oxygen kinetics of the aqueous humor

Utilidade de um analisador sanguíneo de gás para a avaliação cinética do oxigênio no humor aquoso

Purpose:

To measure the partial pressure of oxygen (PO₂) and carbon dioxide (PCO₂) and the pH of aqueous humor (AH) and arterial blood samples from rabbits using a blood gas analyzer.

Methods:

Twenty New Zealand rabbits were anesthetized intramuscularly with ketamine and xylazine and were then allowed to breathe room air. Using a gas blood analyzer, arterial blood and AH samples were analyzed for PO₂, PCO₂, and pH.

Results:

The mean arterial blood pressure was 87.14 ± 15.0 mmHg. The mean blood and AH PO₂ were 95.18 ± 11.76 mmHg and 88.83 ± 9.92 mmHg, the mean blood and AH PCO₂ were 25.86 ± 5.46 mmHg and 29.50 ± 5.36 mmHg, and the mean blood and AH pH were 7.38 ± 0.06 and 7.33 ± 0.09, respectively.

Conclusion:

Conclusions: The blood gas analyzer was easily employed to evaluate the aqueous humor in rabbits. When comparing the results of studies evaluating aqueous PO₂, care should be taken to determine the methods used in these studies.

Aqueous humor; Blood gas analysis; Oxygen/physiology; Carbon dioxide/physiology; Rabbits