OBJECTIVE: Our purpose was to elucidate whether calcium trinatrium diethylenetriaminepentaacetic acid (CaNa3DTPA), a hydrophilic extracelular chelator with some imunological and anti-inflammatory properties, protects skeletal muscle submitted to ischemia/reperfusion. METHODS: Fifty two male Wistar rats were randomly allocated in the following groups:G1-CTAN- control anesthesia whithout surgery. G2-CTCIR - control surgery without ischemia. G3-IRCT and G3-IRDTPA: four hours ischemia and two hours reperfusion. The animals of group 3 were treated with saline and those of the group 4, treated with CaNa3DTPA. Parameters were: foot circumference, rats weight, plasmatic creatine-phosphokinase (CPK), muscular malondialdehyde (MDA), and optic microscopy of muscle cells. RESULTS: There was a significant post-ischemic increase in foot circumference in G3-IRCT. CPK measurements were significantly increased in G3-IRCT and G3-IRDTPA, when compared to G1-CTAN and G2-CTCIR, but there were no differences between G3-IRCT and G3-IRDTPA. MDA was increased in the left no-ischemic muscles in G3-IRCT, when compared to contralateral muscles of G3-IRCT and of G1-CTAN.The histological evaluation showed inflammatory cell infiltrate in G3-IRDTPA and edema in G3-IRCT. The glycogen recovery was similar in both groups. CONCLUSION: There was less edema in the animals with ischemia-reperfusion treated group, but CaNa3DTPA don't protect muscle cells from injury in relations with CPK, MDA managements and histological alterations.
Ischemia; Reperfusion; Skeletal muscle; Diethylenetriaminepentaacetic acid; Rats
