The authors studied the enzymatic alterations (CPK, TGO, e LDH) due to skeletal muscle damage in rats submitted to inferior limb ischemia for 0, 2, 4 and 6 hours. Twenty eight rats were divided into four groups: Group I (control), and Groups II, III and IV (ischemia for 2, 4 and 6 hours, respectively). A blood sample was drawn of the retrocular venous plexus after the ischemia period in all animals. The values obtained (mean±standard deviation) for TGO in Groups I, II, III and IV were, respectively: 20,14±6,76; 59,71±28,91; 88,28±11,17 e 123±52,65 U/l. The values for CPK were: 67,85±62,76; 203±108,71; 237,71±95,06 e 291,71±173,19 U/l. For LDH, we obtained: 414,42±222,47; 526±234,75 e 427,57±273,58 U/l. For the statistical analysis, we used the ANOVA method followed by the Bonferroni’s t test (p<0,05). TGO levels obtained were statistically elevated in the Groups III and IV when compared to the control group. For CPK, there were significant difference between the groups IV and I. LDH levels there were not statisticantly different between the groups. The authors conclude which, after 2, 4 and 6 hours of ischemia, the LDH dosage was not an useful parameter in the prediction of muscle damage. TGO and CPK measures were altered significantly after 4 and 6 hours, respectively being useful in the evaluation of muscle ischemia in rats.
Ischemia; Muscle; Microsurgery; Enzymes; Rats
