Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis

Qian, Yunzhong; Chen, Yi; Wang, Linyang; Tou, Jinfa

doi:10.1590/s0102-865020180070000001

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Acta Cirúrgica Brasileira

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Sumário

ORIGINAL ARTICLES, 1-Experimental Surgery • Acta Cir. Bras. 33 (07) • July 2018 • https://doi.org/10.1590/s0102-865020180070000001 copy

Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis¹ 1 Research performed at Central Laboratory, The Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Purpose:

To investigate the effects of baicalin on inflammatory reaction, oxidative stress and protein kinase D1 (PKD1) and nuclear factor-kappa B (NF-κB) protein expressions in severe acute pancreatitis (SAP) rats.

Methods:

Sixty rats were divided into sham operation, model, and low-, medium- and high-dose baicalin group. SAP model was established in later 4 groups. The later 3 groups were injected with 0.1, 0.2 and 0.4 ml/100 g 5% baicalin injection, respectively. At 12 h, the serum SAP related indexes and inflammatory factors, peripheral blood CD₃ and γδT cell percentages, wet/dry ratio and pancreas ascites volume, oxidative stress indexes and PKD1 and NF-κB protein expressions in pancreatic tissue were determined.

Results:

Compared with model group, in high-dose baicalin group the wet/dry ratio and ascites volume, serum amylase level, phospholipase A₂ activity, TNF-α, IL-1 and IL-6 levels, and pancreatic malondialdehyde level and PKD1 and NF-κB protein expression were significantly decreased (P < 0.05), and peripheral blood CD3 and γδT cell percentages and pancreatic superoxide dismutase and glutathione peroxidase levels were significantly increased (P < 0.05).

Conclusion:

Baicalin can resist the inflammatory reaction and oxidative stress, and down-regulate protein kinase D1 and nuclear factor-kappa B protein expressions, thus exerting the protective effects on severe acute pancreatitis in rats.

Key words:
Pancreatitis; Oxidative Stress; Protein Kinases; NF-kappa B; Rats

Group	Wet/dry ratio	Ascites volume (ml)
Sham operation	3.21±0.65	0.63±0.11
Model	8.45±1.12^a	11.23±2.51^a
Low-dose baicalin	7.21±0.66^a	10.37±1.94^a
Middle-dose baicalin	6.38±0.72^ab	9.16±1.82^a
High-dose baicalin	5.52±0.57^ab	6.29±1.19^abc

Group	Amylase (U/L)	PLA₂ activity (nmol/min·ml)
Sham operation	456.45±67.18	14.56±3.02
Model	1131.17±112.43^a	72.28±14.23 ^a
Low-dose baicalin	1067.36±115.18^a	65.38±10.5 ^a
Middle-dose baicalin	834.18±93.83^ab	34.78±6.38^ab
High-dose baicalin	573.59±101.08^bc	19.31±4.61^bc

Group	TNF-α (pg/ml)	IL-1 (pg/ml)	IL-6 (pg/ml)
Sham operation	16.36±2.28	53.11±11.01	40.42±8.31
Model	128.16±23.03^a	166.83±29.16^a	298.94±45.27^a
Low-dose baicalin	112.48±20.21^a	135.47±24.27^a	202.32±31.83^ab
Middle-dose baicalin	92.71±13.58^a	103.62±18.61^ab	196.17±29.72^ab
High-dose baicalin	68.94±9.17^ab	92.38±15.32^ab	173.05±26.62^ab

Group	CD₃ (%)	γδT (%)
Sham operation	71.41±6.16	6.33±1.02
Model	57.62±5.25^a	3.04±0.63^a
Low-dose baicalin	58.37±6.73^a	3.35±0.72^a
Middle-dose baicalin	59.25±4.43^a	4.17±0.53^a
High-dose baicalin	63.52±6.26^abc	5.01±0.95^abc

Group	SOD (U/mgprot)	GSH-Px (U/mgprot)	MDA (mmol/mgprot)
Sham operation	174.45±19.73	131.12±14.56	1.39±0.67
Model	126.12±18.01^a	76.57±11.03^a	7.31±1.23^a
Low-dose baicalin	131.47±14.36^a	86.32±10.12^a	6.34±0.98^a
Middle-dose baicalin	147.73±15.12^ab	104.17±12.93^abc	4.56±0.72^ab
High-dose baicalin	162.19±17.02^abc	117.95±13.08^bc	3.35±0.39^abc

Group	PKD1/β-actin	NF-κB/β-actin
Sham operation	0.04±0.01	0.25±0.04
Model	0.56±0.07^a	0.93±0.08^a
Low-dose baicalin	0.48±0.06^a	0.87±0.09^a
Middle-dose baicalin	0.23±0.04^abc	0.57±0.07^ab
High-dose baicalin	0.12±0.02^abc	0.42±0.05^bc

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[1] Correspondence: Dr Yi Chen Department of General Surgery, The Children’s Hospital School of Medicine, Zhejiang University 57 Bamboo Alley, Hangzhou 310052 China Phone: +86-571-88873023 6193006@zju.edu.cn

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Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis1 1 Research performed at Central Laboratory, The Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Abstract

Purpose:

Methods:

Results:

Conclusion:

Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis¹ 1 Research performed at Central Laboratory, The Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou, China.