Is the combination of fat grafts and platelet rich plasma effective in rats?

This is an experimental, randomized and blinded study, which involved 47 rats. Fat was harvested from the inguinal region and grafted to the cranial region. The experimental group consisted of PRP-enriched fat grafts (n=22) whilst the control group consisted of fat graft only (n=25). After a 100-day period, the animals were euthanised and the fat grafts were analyzed using scores from 0 (absent) to 4 (abundant), in optical microscopy by two independent and blinded pathologists.

RESULTS:

Regarding fat graft cell viability, the PRP group scored moderate/abundant in 63% of cases and the fat graft only group scored absent/slight in 72% of cases (p=0.03). The PRP group also presented lower fat necrosis scores when compared to the fat graft only group (p=0.03). Tumors (dermoid cysts) within the fat grafts were observed in three animals in which the grafts were mixed with PRP.

CONCLUSION:

Platelet-rich plasma improves the viability and integration of fat grafts in rats, but more studies are needed to fully understand the exact mechanisms that lead to this improvement and assess the safety of the method for use in humans.

Key words:
Adipose Tissue; Graft Survival; Platelet-Rich Plasma; Rats

	Evaluator 1			p	RR (95% CI)		Evaluator 2				p	RR (95% CI)
	Fat + PRP			Fat						Fat + PRP		Fat
	n=22	%	n=25	%			n=22	%	n=25	%
Fat cells
Sparse	9	40.9	18	72.0	0.03	0.26 (0.08 - 0.91)	8	36.4	18	72.0	0.010	0.22 (0.06 - 0.76)
Abundant	13	59.1	7	28.0	0.03	0.26 (0.08 - 0.91)	14	63.6	7	28.0	0.010	0.22 (0.06 - 0.76)
Area of necrosis
Sparse	13	59.1	7	28.0	0.03	3.71 (1.09 - 12.56)	14	63.6	7	28.0	0.014	4.50 (1.31 - 15.42)
Abundant	9	40.9	18	72.0	0.03	3.71 (1.09 - 12.56)	8	36.4	18	72.0	0.014	4.50 (1.31 - 15.42)
Local inflammation
Sparse	19	86.4	16	64.0	0.07	3.56 (0.82 - 15.43)	20	90.9	16	64.0	0.03	5.62 (1.06 - 29.79)
Abundant	3	13.6	9	36.0	0.07	3.56 (0.82 - 15.43)	2	9.1	9	36.0	0.03	5.62 (1.06 - 29.79)

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

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[1] Correspondence: Ruffo Freitas-Junior Programa de Mastologia, Hospital das Clínicas-UFG 1ª Avenida, s/n, Setor Universitário. 74605-050 Goiânia-GO Brasil Tel.: (55 62)3945-4769 ruffojr@terra.com.br

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Is the combination of fat grafts and platelet rich plasma effective in rats?1 1 Research performed at Goiânia Mastology Research Network and Mastology Program, Teaching Hospital, Universidade Federal de Goiás, Goiania-GO, Brazil.

ABSTRACT

PURPOSE:

METHODS:

RESULTS:

CONCLUSION:

Is the combination of fat grafts and platelet rich plasma effective in rats?^¹ 1 Research performed at Goiânia Mastology Research Network and Mastology Program, Teaching Hospital, Universidade Federal de Goiás, Goiania-GO, Brazil.