Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model

To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats.

Methods

Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue.

Results

Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups.

Conclusion

Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression.

Ethanol; Erectile Dysfunction; Apoptosis; MicroRNAs; Rats

Authorship

Rogério José de Azevedo Meirelles

PhD, Department of Surgery and Anatomy, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirao Preto-SP, Brazil. Acquisition of data.Universidade de São PauloBrazilRibeirao Preto, SP, BrazilPhD, Department of Surgery and Anatomy, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirao Preto-SP, Brazil. Acquisition of data.

http://orcid.org/0000-0001-9208-7120

Fermino Sanches Lizarte Neto

PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Technical procedures.USPBrazilRibeirao Preto, SP, BrazilPhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Technical procedures.

http://orcid.org/0000-0002-0046-7300

Mucio Luiz de Assis Cirino

Fellow PhD degree, Postgraduate Program in Clinical Surgical, Department of Surgery and Anatomy, Surgical Clinic Program, FMRP-USP, Ribeirao Preto-SP, Brazil. Acquisition of data.USPBrazilRibeirao Preto, SP, BrazilFellow PhD degree, Postgraduate Program in Clinical Surgical, Department of Surgery and Anatomy, Surgical Clinic Program, FMRP-USP, Ribeirao Preto-SP, Brazil. Acquisition of data.

http://orcid.org/0000-0001-7872-8331

Paulo Cezar Novais

PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Postgarduate Program in Structural and Functional Interactions in Rehabiliattion, Universidade de Marília (UNIMAR), Marilia-SP, Brazil. Histopathological examinations.USPBrazilRibeirao Preto, SP, BrazilPhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Postgarduate Program in Structural and Functional Interactions in Rehabiliattion, Universidade de Marília (UNIMAR), Marilia-SP, Brazil. Histopathological examinations.

Postgarduate Program in Structural and Functional Interactions in Rehabiliattion, Universidade de Marília, Marilia, SP, BrazilUniversidade de MaríliaBrazilMarilia, SP, BrazilPostgarduate Program in Structural and Functional Interactions in Rehabiliattion, Universidade de Marília, Marilia, SP, Brazil

http://orcid.org/0000-0001-8372-6293

Isabella Stracieri Gula

Graduate student, School of Medicine, Universidade de Ribeirão Preto (UNAERP), Brazil. Acquisition of data.Universidade de Ribeirão PretoBrazilBrazilGraduate student, School of Medicine, Universidade de Ribeirão Preto (UNAERP), Brazil. Acquisition of data.

http://orcid.org/0000-0003-0797-9525

Jairo Pinheiro da Silva

PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Professor, Faculdade de Taquaritinga (FTGA), Brazil. Manuscript preparation.USPBrazilRibeirao Preto, SP, BrazilPhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Professor, Faculdade de Taquaritinga (FTGA), Brazil. Manuscript preparation.

Faculdade de Taquaritinga, BrazilFaculdade de TaquaritingaBrazilBrazilFaculdade de Taquaritinga, Brazil

http://orcid.org/0000-0002-5213-8525

Maria de Fátima Galli Sorita Tazzima

PhD, Assistant Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Manuscript writing.USPBrazilRibeirao Preto, SP, BrazilPhD, Assistant Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Manuscript writing.

http://orcid.org/0000-0002-5844-7310

Valéria Paula Sassoli Fazan

PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Manuscript writing.USPBrazilRibeirao Preto, SP, BrazilPhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Manuscript writing.

http://orcid.org/0000-0003-1293-5308

Marina Toledo Durand

PhD, UNAERP, Ribeirao Preto-SP, Brazil. Manuscript writing.UNAERPBrazilRibeirao Preto, SP, BrazilPhD, UNAERP, Ribeirao Preto-SP, Brazil. Manuscript writing.

http://orcid.org/0000-0002-5625-4662

Daniela Pretti da Cunha Tirapelli

PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Final approval.USPBrazilRibeirao Preto, SP, BrazilPhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Final approval.

http://orcid.org/0000-0002-1886-4988

Camila Albuquerque Melo de Carvalho

PhD, Department of Surgery and Anatomy, FMRP-USP, and Professor, Centro Universitário Barão de Mauá, Ribeirao Preto-SP, Brazil. Manuscript writing.USPBrazilBrazilPhD, Department of Surgery and Anatomy, FMRP-USP, and Professor, Centro Universitário Barão de Mauá, Ribeirao Preto-SP, Brazil. Manuscript writing.

Centro Universitário Barão de Mauá, Ribeirao Preto, SP, BrazilCentro Universitário Barão de MauáBrazilRibeirao Preto, SP, BrazilCentro Universitário Barão de Mauá, Ribeirao Preto, SP, Brazil

http://orcid.org/0000-0003-2325-2844

Bruno César Schimming

PhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Associate Professor, Department of Bioscience, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Critical revision.USPBrazilRibeirao Preto, SP, BrazilPhD, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, and Associate Professor, Department of Bioscience, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Critical revision.

Department of Bioscience, Universidade Estadual Paulista, Botucatu, SP, BrazilUniversidade Estadual PaulistaBrazilBotucatu, SP, BrazilDepartment of Bioscience, Universidade Estadual Paulista, Botucatu, SP, Brazil

http://orcid.org/0000-0002-0837-9612

Carlos Augusto Fernandes Molina

http://orcid.org/0000-0002-5118-778X

Silvio Tucci Junior

http://orcid.org/0000-0002-7656-0181

Luis Fernando Tirapelli

http://orcid.org/0000-0002-2251-1106

Correspondence: Luis Fernando Tirapelli Avenida Bandeirantes, 3900 14049-900 Ribeirão Preto-SP Brasil Tel.: (55 16)3315-3305 lab.biomol.cirurgia@fmrp.usp.br

Conflict of interest: none

SCIMAGO INSTITUTIONS RANKINGS

Faculdade de Taquaritinga, BrazilFaculdade de TaquaritingaBrazilBrazilFaculdade de Taquaritinga, Brazil

PhD, UNAERP, Ribeirao Preto-SP, Brazil. Manuscript writing.UNAERPBrazilRibeirao Preto, SP, BrazilPhD, UNAERP, Ribeirao Preto-SP, Brazil. Manuscript writing.

Centro Universitário Barão de Mauá, Ribeirao Preto, SP, BrazilCentro Universitário Barão de MauáBrazilRibeirao Preto, SP, BrazilCentro Universitário Barão de Mauá, Ribeirao Preto, SP, Brazil

Figures

Figures (8)

Thumbnail

Figure 1
Cross-sectional photomicrograph of the rat penis where the two corpus cavernosum (CC) covered by the tunica albuginea ( arrow ) can be identified and contain a large number of cavernous ( blank ) spaces within it; ventrally the urethra (U) inside the spongy body and the dorsal deep vein of the penis (VDP). Masson’s trichrome, x50.

Thumbnail

Figure 2
Photomicrography showing detail of some connective trabeculae composed mainly of collagen fibers (CF) in blue and by subendothelial smooth muscle ( thinner arrows ). In the center a large cavernous space (EC) containing red blood cells inside ( red ) and endothelial ( thick arrow ). Masson’s trichrome, x400.

Thumbnail

Figure 3
Average area occupied by the smooth muscle fibers of the corpus cavernous of each animal from A and C groups (Two- way ANOVA, p=0.0002).

Thumbnail

Figure 4
Average area occupied by collagen fibers corpus cavernosum of the C and A groups (Mann-Whitney test, p=0.1988).

Thumbnail

Figure 5
Average area occupied by gaps or cavernous spaces of the corpus cavernosum in groups C and A (Mann-Whitney test, p=0.1583).

Thumbnail

Figure 6
Average of the protein expression of caspase 3 in the corpus cavernosum of C and A groups (Two-way ANOVA, p<0.0001).

Thumbnail

Figure 7
Gene expression of miRNA -21 in the serum in groups C and A (Mann- Whitney test, p=0.2135).

Thumbnail

Figure 8
Gene expression of miRNA -21 in the corpus cavernosum tissue in groups C and A (Mann- Whitney test, p=0.864).

Figure 1 Cross-sectional photomicrograph of the rat penis where the two corpus cavernosum (CC) covered by the tunica albuginea ( arrow ) can be identified and contain a large number of cavernous ( blank ) spaces within it; ventrally the urethra (U) inside the spongy body and the dorsal deep vein of the penis (VDP). Masson’s trichrome, x50.

Figure 2 Photomicrography showing detail of some connective trabeculae composed mainly of collagen fibers (CF) in blue and by subendothelial smooth muscle ( thinner arrows ). In the center a large cavernous space (EC) containing red blood cells inside ( red ) and endothelial ( thick arrow ). Masson’s trichrome, x400.

Figure 3 Average area occupied by the smooth muscle fibers of the corpus cavernous of each animal from A and C groups (Two- way ANOVA, p=0.0002).

Figure 4 Average area occupied by collagen fibers corpus cavernosum of the C and A groups (Mann-Whitney test, p=0.1988).

Figure 5 Average area occupied by gaps or cavernous spaces of the corpus cavernosum in groups C and A (Mann-Whitney test, p=0.1583).

Figure 6 Average of the protein expression of caspase 3 in the corpus cavernosum of C and A groups (Two-way ANOVA, p<0.0001).

Figure 7 Gene expression of miRNA -21 in the serum in groups C and A (Mann- Whitney test, p=0.2135).

Figure 8 Gene expression of miRNA -21 in the corpus cavernosum tissue in groups C and A (Mann- Whitney test, p=0.864).

How to cite

copy

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org