The spontaneous incidence of gastric cancer in rat is extremely low. Furthermore, the development of this type of cancer induced by chemical carcinogens, fundic resection, vagotomy or reflux is very low, erratic and takes several months. The present work developed a relatively easy and reliable method for topical implantation of a fast growth tumor in the rat gastric mucosa. Cells from Walker tumor were injected through a canula in the rat stomach previously injured by clamping the mucosa in three different gastric regions. The tumors were found 100% in the large curvature, 80% in the small curvature and 20% in the esophagogastric juction. The surviving time of the inoculated animals was 13,2±1,92 days. The neoplastic tissue showed a rapid and constant growth pattern and it has allowed to inoculate a large series of rats.
Experimental gastric tumor; Drug evaluation tumor model; Walker tumor
