Effects of different periods of gastric ischemia on liver as a remote organ

Thirty male rabbits were studied, distributed in 3 groups (n=10). Group 1: ligature and section of the gastric vasculature and removal of the liver after three hours; Group 2: ligature and section of the gastric vasculature and removal of the liver after 6 hours; Group 3: ligature and section of the gastric vasculature and removal of the liver after 12 hours. Blood samples were collected immediately before surgery and after the determined time of ischemia in each group to evaluate the hepatic function. After the death of the rabbits, the liver was removed for macro and microscopic study.

Results:

An increase in aminotransferases and bilirubin occurred in groups 2 and 3. Total protein and albumin diminished in all of the animals. All of the rabbits from groups 2 and 3 presented hepatocellular necrosis.

Conclusion:

The devascularization of the stomach for a period of above three hours is associated with hepatic morphological and functional disorders.

Key words:
Stomach; Ischemia; Inflammation; Liver; Rabbits

GROUP/MICROSCOPIC CHANGES	VACUOLAR HYDROPIC DEGENERATION n (%)	DILATATION OF BLOOD VESSELS n (%)	CONGESTION OF BLOOD VESSELS n (%)	HEPATOCELLULAR NECROSIS n (%)	LEUKOCYTE INFILTRATION n (%)
1	10 (100%)	10 (100%)	10 (100%)	2 (20%)	-
2	8 (80%)	8 (80%)	8 (80%)	10 (100%)	-
3	10 (100%)	10 (100%)	10 (100%)	10 (100%)	8 (80%)

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[1] Correspondence:
Andy Petroianu Avenida Afonso Pena, 1626/1901 30.130-100 Belo Horizonte - MG Brasil Tel.: (55 31)98884-9192 / 3409-9948 petroian@gmail.com

VARIABLES	GROUPS	PREOPERATIVE	POST-ISCHEMIA	PRE- AND POST-VARIATION
ALT (IU/l)	1	135.8 ± 23.2 A	158.8 ± 19.8 A	23.00a
	2	115.6 ± 16.0 A	151.0 ± 46.2 B	35.40 a
	3	96.8 ± 20.0 A	135.0 ± 45.6 B	-61.80 b
AST (IU/l)	1	32.4 ± 12.5 A	20.2 ± 12.5 A	-12.20 a
	2	40.2 ± 6.3 A	138.0 ± 264.0 A	93.80 a
	3	44.8 ± 10.6 A	776.2 ± 320.4 B	731.40 a
ALP (IU/l)	1	99.4 ±23.6 A	104.8 ± 25.9 A	5.40 a
	2	141.6 ± 44.2 A	138.0 ± 21.5 A	-3.60 a
	3	146.0 ± 41.6 A	290.0 ± 194.2 A	144.00 a
GGT (IU/l)	1	5.9 ± 4.8 A	6.4 ± 3.6 A	0.50 a
	2	5.4 ± 2.2 A	5.9 ± 2.0 A	0.54 a
	3	5.2 ± 2.9 A	17.6 ± 13.4 A	12.26 a
TP (g/dl)	1	8.6 ± 0.8 A	6.8 ± 0.3 B	-1.60 a
	2	7.3 ± 0.5 A	6.4 ± 0.3 B	-1.00 a
	3	7.0 ±0.5 A	5.6 ± 0.4 B	-1.40 a
ALBUMIN (g/dl)	1	4.2 ± 0.2 A	3.3 ± 0.4 B	-0.90 a
	2	3.7 ± 0.2 A	2.5 ± 0.3 B	-1.22 a
	3	3.7 ± 0.9 A	2.4 ± 0.5 B	-1.28a
DIRECT BILIRUBIN (mg/dl)	1	0.26 ± 0.1 A	0.2 ± 0.1 B	-0.05 a
	2	0.5 ± 0.3 A	0.6 ± 0.4 A	0.10 ab
	3	0.2 ± 0.03 A	0.3 ± 0.1 B	0.16 b
INDIRECT BILIRUBIN (mg/dl)	1	0.5 ± 0.2 A	0.5 ± 0.3 A	-0.01 a
	2	1.0 ± 0.8 A	1.3 ± 0.6 B	0.03 a
	3	0.9 ± 0.4 A	1.9 ± 1.5 A	1.06 a

Brasil

Brasil

Effects of different periods of gastric ischemia on liver as a remote organ1 1 Research performed at Laboratory of Experimental Surgery, Department of Surgery, Faculty of Medicine, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil.

Abstract

Purpose:

Methods:

Results:

Conclusion:

Effects of different periods of gastric ischemia on liver as a remote organ¹ 1 Research performed at Laboratory of Experimental Surgery, Department of Surgery, Faculty of Medicine, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil.