Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats

Mousavi, Ghafour

doi:10.1590/S0102-865020150080000005

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Original Articles • Acta Cir. Bras. 30 (8) • Aug 2015 • https://doi.org/10.1590/S0102-865020150080000005 copy

Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats¹ 1 Reseach performed at Surgery and Radiology Section, Department of Clinical Sciences, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

Authorship SCIMAGO INSTITUTIONS RANKINGS

PURPOSE:

To evaluate the effect of Black cumin (Nigella sativa Linn.) pre-treatment on renal ischemia/reperfusion (I/R) induced injury in the rats.

METHODS:

A total of 40 male Wistar rats were randomly allocated into five equal groups including Sham, I/R model and three I/R+ Black cumin (0.5, 1 and 2%)-treated groups. I/R groups' kidneys were subjected to 60 min of global ischemia at 37°C followed by 24 h of reperfusion. At the end of reperfusion period, the rats were euthanized. Superoxide dismutase, catalase and glutathione peroxidase activities as well as reduced glutathione and renal malondialdehyde contents were determined in renal tissues. Kidney function tests and histopathological examination were also performed.

RESULTS:

High serum creatinine, blood urea nitrogen and uric acid as well as malondialhehyde (MDA) levels, and low antioxidant enzyme activities were observed in I/R rats compared to the sham rats. Pre-treatment with Black cumin for three weeks prior to IR operation improved renal function and reduced I/R induced renal inflammation and oxidative injury. These biochemical observations were supported by histopathological test of kidney sections.

CONCLUSION:

Black cumin significantly prevented renal ischemia/reperfusion induced functional and histological injuries.

Nigella sativa; Ischemia; Reperfusion; Kidney; Rats

Group	Scr (mg/dL)	BUN (mg/dL)	Uric Acid (mg/dL)
Sham	1.55 ± 0.09	61.32 ± 5.8	0.79 ± 0.11
I/R model	4.23 ± 0.21^a	142.95 ± 12.75^a	1.82 ± 0.18^a
I/R+Black cumin 0.5%	3.72 ± 0.18	131.87 ± 9.24	1.71 ± 0.14
I/R+Black cumin 1%	2.84 ± 0.11^b	102. 66 ± 7.32^b	1.32 ± 0.12^b
I/R+Black cumin 2%	1.95 ± 0.08^c	72.64 ± 4.3^c	0.92 ± 0.15^c

Group	Water intake (mL)	Urinary excretion (mL)	Kidney–body weight ratio
Sham	33.8 ± 1.4	10.53 ± 0.95	0.0037 ± 0.0001
I/R model	18.4 ± 5.3^a	4.3 ± 0.42^a	0.0048 ± 0.0001^a
I/R+Black cumin 0.5%	21.1 ± 3.6	5.5 ± 0.63	0.0045 ± 0.0001
I/R+Black cumin 1%	26.7 ± 2.8^b	7.4 ± 0.81^b	0.0043 ± 0.0001^b
I/R+Black cumin 2%	32.5 ± 2.2^c	9.6 ± 0.85^c	0.0040 ± 0.0001^c

Group	MDA (nmol/mg protein)	GSH (μmol/g protein)	SOD (U/mg protein)	CAT (U/mg protein)	GSH-Px (U/mg protein)
Sham	4.78 ± 0.22	2.79 ± 0.13	164.21 ± 14.27	44.14 ± 3.63	43.62 ± 2.67
I/R model	9.10 ± 0.41^a	1.17 ± 0.07^a	82.29 ± 4.26^a	22.13± 2.15^a	25.35±0.54^a
I/R+Black cumin 0.5%	8.62 ± 0.48	1.47 ± 0.18	90.73 ± 5.84	26.31 ± 2.78	28.14 ± 1.65
I/R+Black cumin 1%	6.81 ± 0.59^b	1.93 ± 0.11^b	145.57 ± 13.72^b	34.12 ± 3.16^b	33.49 ± 1.78^b
I/R+Black cumin 2%	5.44 ± 0.26^c	2.42 ± 0.15^c	161.42 ± 10.95^c	39.81 ±3.58^c	39.72± 1.44^c

Group	Cellular vacuolation	Inflammation, congestion, edema and hemorrhage	Tubular dilation	Epithelial necrosis	Hyaline cast
Sham	-	-	-	-	-
I/R model	+++	+++	+++	+++	+++
I/R+Black cumin 0.5%	+++	++	++	++	++
I/R+Black cumin 1%	++	+	+	+	+
I/R+Black cumin 2%	+	-	+	-	-

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[1] Correspondence: Ghafour Mousavi Department of Clinical Sciences College of Veterinary Medicine Tabriz Branch, Islamic Azad University Tabriz, Iran Phone: +989144010811 Fax: +984116374611 gh_mousavi@iaut.ac.ir

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Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats1 1 Reseach performed at Surgery and Radiology Section, Department of Clinical Sciences, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

PURPOSE:

METHODS:

RESULTS:

CONCLUSION:

Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats¹ 1 Reseach performed at Surgery and Radiology Section, Department of Clinical Sciences, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.