The effect of probiotics on ıntestinal motility in an experimental short bowel model

Metin, Mehmet; Altun, Ahmet; Köylüoğlu, Gökhan

doi:10.1590/s0102-865020200080000004

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Acta Cirúrgica Brasileira

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Sumário

ORIGINAL ARTICLE, Experimental Surgery • Acta Cir. Bras. 35 (8) • 2020 • https://doi.org/10.1590/s0102-865020200080000004 copy

The effect of probiotics on ıntestinal motility in an experimental short bowel model ¹ 1 Research performed at Department of Pediatric Surgery, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Purpose

To investigate the effect of probiotics on spontaneous contractions of smooth muscle isolated from jejunum and ileum of rat model.

Methods

Four rat groups were created (n=8, in each) including control (Group 1), control+probiotic (Group 2), short bowel (Group 3), and short bowel+probiotic (Group 4). Groups 1 and 2 underwent sham operation, Groups 3 and 4 underwent massive bowel resection. Bifidobacterium Lactis was administered in Groups 2 and 4 daily (P.O.) for three weeks. On postoperative week 3, rats were sacrificed, and jejunum and ileum smooth muscle were isolated for organ bath. Muscle contraction changes were analyzed before and after addition of antagonists.

Results

Short bowel group exhibited increased amplitude and frequency of spontaneous contractions. The addition of probiotics significantly decreased enhanced amplitude and frequency of bowel contraction in short bowel group and returned to control values. L-NNA increased amplitude and frequency of contractions in all groups. While indomethacin and nimesulide increased the amplitude in all groups, the frequency was only increased in jejunum. Hexamethonium and tetrodotoxin did not change the contraction characteristics in all groups.

Conclusion

We suggest that early use of probiotics may significantly regulate bowel motility, and accordingly improve absorption of nutrients in short bowel syndrome.

Short Bowel Syndrome; Gastrointestinal Motility; Probiotics; Amplitude; Rats

	Control	Short intestine	Pro + control	Pro + Short intestine	P
Ileum	2.54 ± 0.20	2.80 ± 0.24	2.38 ± 0.27	2.72 ± 0.24	0.095
Jejunum	2.05 ± 0.26	1.96 ± 0.24	1.90 ± 0.22	2.00 ± 0.25	0.467

	Control	Short intestine	Pro + control	Pro + Short intestine	P values	Post-hoc P values
Ileum	80.6 ± 6.8	120.6 ± 7.0*	72.2 ± 6.0	96.8 ± 7.6	<0.001*	1-2; <0.001* 1-3; 0.06 1-4; 0.053
Jejunum	60.7 ± 4.5	80.8 ± 5.0*	55.2 ± 4.7	66.2 ± 4.3	0.001*	1-2; <0.001* 1-3; 0.093 1-4; 0.075

	Control	Short intestine	Pro + control	Pro + Short intestine	P	Post-hoc P values
Ileum	27.2 ± 4,2	42.8 ± 3.9*	24.0 ± 3.6	32.6 ± 4.1	<0.001*	1-2; <0.001* 1-3; 0.372 1-4; 0.063
Jejunum	20.2 ± 3,0	29.2 ± 3.2*	17.8 ± 3.4	23.8 ± 3.3	0.010*	1-2; <0.001* 1-3; 0.471 1-4;0.117

Ileum	Control	Short intestine	Pro + control	Pro + Short intestine
Vehicle	80.6 ± 6.6	120.6 ± 7.0	72.2 ± 6.6	96.8 ± 7.6
L-NNA	124.2 ± 8.2*	168.8 ± 8.1*	94.2 ± 7.1*	134.2 ± 7.0*
Indomethacin	112.0 ± 7.2*	156.8 ± 7.2*	90.6 ± 6.4*	128.2 ± 7.2*
Nimesulide	102.8 ± 6.9*	148.2 ± 6.6*	87.2 ± 5.4*	118.4 ± 6.8*
Hexamethonium	70.9 ± 6.8	110.5 ± 7.0	66.6 ± 5.0	89.7 ± 6.4
Tetrodotoxin	72.3 ± 6.5	107.2 ± 6.4	64.2 ± 5.2	82.4 ± 6.3
P values	<0.001*	<0.001*	0.002*	<0.001*
Post hoc P values	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*
	1-3; <0.001*	1-3; <0.001*	1-3; <0.001*	1-3; <0.001*
	1-4; <0.001*	1-4; <0.001*	1-4; <0.001*	1-4; <0.001*

Jejunum	Control	Short intestine	Pro + control	Pro + Short intestine
Vehicle	60.7 ± 4.5	80.8 ± 5.0	55.2 ± 4.7	66.2 ± 4.3
L-NNA	88.8 ± 5.2*	113.6 ± 6.1*	77.4 ± 4.5*	100.8 ± 5.2*
Indomethacin	84.2 ± 4.8*	106.3 ± 5.2*	72.2 ± 4.3*	93.4 ± 4.6*
Nimesulide	80.7 ± 4.3*	100.2 ± 4.8*	69.8 ± 4.6*	88.8 ± 4.4*
Hexamethonium	54.1 ± 4.0	73.4 ± 4.3	48.9 ± 4.0	59.0 ± 4.5
Tetrodotoxin	52.6 ± 4.4	70.0 ± 5.2	45.4 ± 4.7	56.9 ± 4.4
P values	<0.001*	<0.001*	<0.001*	<0.001*
Post hoc P values	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*
	1-3; <0.001*	1-3; <0.001*	1-3; <0.001*	1-3; <0.001*
	1-4; <0.001*	1-4; <0.001*	1-4; <0.001*	1-4; <0.001*

Ileum	Control	Short intestine	Pro + control	Pro + Short intestine
Vehicle	27.2 ± 4.2	42.8 ± 3.9	24.0 ± 3.6	32.6 ± 4.1
L-NNA	44.8 ± 4.5*	56.6 ± 4.0*	37.2 ± 3.5*	47.2 ± 4.0*
Indomethacin	32.2 ± 4.0	45.4 ± 3.8	27.2 ± 3.2	36.8 ± 4.0
Nimesulide	30.7 ± 4.1	44.4 ± 3.6	25.9 ± 3.3	34.2 ± 3.9
Hexamethonium	24.4 ± 3.8	36.8 ± 4.5	20.6 ± 3.5	29.0 ± 4.1
Tetrodotoxin	25.0 ± 4.0	35.7 ± 4.0	18.8 ± 4.0	27.0 ± 4.2
P values	<0.001*	<0.001*	<0.001*	<0.001*
Post hoc P values	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*

Jejunum	Control	Short intestine	Pro + control	Pro + Short intestine
Vehicle	20.2 ± 3.0	29.2 ± 3.2	17.8 ± 3,4	23.8 ± 3.3
L-NNA	30.6 ± 3.2*	44.5 ± 4.0*	28.0 ± 3.0*	37.2 ± 3.4*
Indomethacin	27.2 ± 3.0*	40.0 ± 4.0*	25.2 ± 2.9*	34.0 ± 3.0*
Nimesulide	26.8 ± 3.2*	38.2 ± 3.9*	24.4 ± 3.1*	32.2 ± 2.8*
Hexamethonium	17.3 ± 2.8	26.0 ± 3.1	15.0 ± 2.8	19.8 ± 3.0
Tetrodotoxin	15.4 ± 3.0	24.2 ± 3.7	14.1 ± 2.6	18.8 ± 2.9
P values	<0.001*	<0.001*	<0.001*	<0.001*
Post hoc P values	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*	1-2; <0.001*
	1-3; <0.001*	1-3; <0.001*	1-3; <0.001*	1-3; <0.001*
	1-4; 0.003*	1-4; <0.001*	1-4; 0.003*	1-4; <0.001*

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[1] Correspondence: Mehmet Metin. Hitit Üniversitesi Erol Olçok Eğitim ve Araştırma Hastanesi. Bahçelievler Mh., Çamlık Cd., No:2, Postal Code: 19200 Merkez/Çorum Turkey. Phone: +90 (364) 223 03 00. mehmet-mtn@hotmail.com

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The effect of probiotics on ıntestinal motility in an experimental short bowel model 1 1 Research performed at Department of Pediatric Surgery, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.

Abstract

Purpose

Methods

Results

Conclusion

The effect of probiotics on ıntestinal motility in an experimental short bowel model ¹ 1 Research performed at Department of Pediatric Surgery, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.