This research deals with the effects of exogenous growth regulators on leaf osmotic potential of 'Davis' soybean. To study the influence of growth substances on osmotic potential (2-chloroethyl) trimethylammonium chloride (CCC) at concentration of 2,000 ppm, succinic acid-2,2-dimethylhydra-zide (SADH) 4,000 ppm, gibberellic acid (GA) 100 ppm, and indolylacetic acid acid (IAA) 100 ppm were applied. The leaf osmotic potential established every day during three days showed that plants treated with SADH presented lower (negative) osmotic potential.