The effect of growth regulators on the germination and initial development of sugar cane shoots submitted to high temperature treatment by the moist method was studied. The shoots were treated during 1 hour with indoleacetic´acid (IAA) and naphthaleneacetic acid (NAA) at the concentrations of 10, 25, 50 and 100 ppm, with indolebutyric acid (IBA) at the concentrations of 10 and 25 ppm and with pure water (control). Immediately after the treatment, the shoots were planted in sand germinators. The results showed that treatment with IBA 10 ppm promote a tendency to induce germination and roots development. The root system development was not affected by other treatments, but the development of the aerial portion was adversely affected by NAA 100 ppm. The results showed that, after 60 days, the number of emerged shoots was significantly lower than that of the control in the plots treated with 100 ppm of NAA.