This paper deals with experiments designed to study the influence of several external factors on the uptake both of Ca+² and H2PO4 - by excised soybean roots. The main conclusions are as follows: 1) Ca uptake does not follow the usual Michaelian kynetics, the evidence suggesting the operation of passive mechanism; 2) R uptake is metabolic, a single mechanism being in operation; 3) pH has a strong effect both on Ca and R absorption; 4) The use both of lower temperature (O0C) and of respiratory poisons (cyanide) inibited phosphate uptake but had no effect on calcium absorption. 5) The obsorption of Ca+2 was lowered in the presence of Mg+² but not in that of K+; nitrate ions had no detrimental or sinergistic offect on the absorption of phosphate.