Change in leukocyte by acute toxicological stress of diesel oil in juveniles of Nile tilapia

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ABSTRACT:

This study aimed to evaluate the stress and changes in white blood cell count of juveniles Oreochromis niloticus subjected to acute toxicity of diesel oil. The toxicity was evaluated in a completely randomized design with five dilutions (20, 25, 30, 35 and 40%) and one control, both with three replicates. For the assessment of blood glucose levels and changes in white blood cell count, blood was drawn by caudal puncture of the dying fish. It was concluded that diesel oil can present risks to aquatic ecosystem, causing toxicity and physiological changes to fish.

KEYWORDS:
physiological changes; fish; derived oil; leukogram

	Diluições de óleo diesel (média ± DP)
	Controle	20%	25%	30%	35%	40%
Leucócitos (x10³.µL^-1)	42,80 ± 4,57b	42,60 ± 6,35b	47,70 ± 9,93ab	49,90 ± 3,99ab	50,90 ± 5,04ab	58,40 ± 6,82ª
Linfócitos (x10³.µL^-1)	31,20 ± 4,41a	31,4 ± 3,84a	30,40 ± 3,51a	27,90 ± 2,06ab	24,30 ± 3,37b	24,10 ± 2,44b
Monócitos (x10³.µL^-1)	5,60 ± 1,24d	4,75 ± 1,81d	8,54 ± 3,84cd	12,50 ± 1,99bc	16,60 ± 2,60b	24,00 ± 3,15ª
Neutrófilos (x10³.µL^-1)	6,92 ± 0,81a	6,33 ± 4,46a	8,62 ± 3,15a	9,40 ± 4,20a	9,75 ± 3,57a	8,77 ± 3,43a
Basófilos (x10³.µL^-1)	0,09 ± 0,07a	0,09 ± 0,07a	0,13 ± 0,10a	0,09 ± 0,05a	0,15 ± 0,10a	0,14 ± 0,06a

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Instituto Biológico Av. Conselheiro Rodrigues Alves, 1252 - Vila Mariana - São Paulo - SP, 04014-002 - São Paulo - SP - Brazil
E-mail: arquivos@biologico.sp.gov.br

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[TFN1] DP: desvio padrão; letras diferentes nas linhas indicam diferença significativa pelo teste de Tukey (5%).