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LABORATORIAL DIAGNOSIS OF LEPTOSPIROSIS IN ONE DOG USING DIFFERENT ASSAYS

ABSTRACT

The purpose of this study was to describe the clinical features of a dog with leptospirosis suspicion, and results of specific and complementary laboratorial tests for leptospirosis. Anamnesis revealed that the animal presented vomiting, difficulty to apprehend both solid and liquid food. In the clinical examination, there was verified dehydration from 5 to 7%, hematoemesis, melena, diarrhea, tong necrosis, sialorrhea and difficulty swallowing. All the specific tests, bacteriologic, direct examination of urine in dark field microscopy, microscopic agglutination test and indirect immunoperoxidase, were positive. The Leptospira strain isolated was identified as serovar Canicola. The complementary tests revealed leukocytosis with neutrophilia, hypocalcemia, hypalbuminemia and blood values of urea, creatinine and phosphates over the normal values while urinalysis showed proteinuria, leukocyturia and presence of kidney cells. Kidney lesions found in HE were chronic interstitial nephritis and glomerulitis with thickened basement membrane. The clinical signs and the results of laboratorial tests found in this study indicated to predominantly to renal feature, consistent with an infection by serovar Canicola.

KEY WORDS
Leptospirosis; serovar Canicola; diagnosis; dog; kidney

Instituto Biológico Av. Conselheiro Rodrigues Alves, 1252 - Vila Mariana - São Paulo - SP, 04014-002 - São Paulo - SP - Brazil
E-mail: arquivos@biologico.sp.gov.br