PORCINE PARVOVIRUS ISOLATION FROM FETAL TISSUES OF SWINE FEMALES WITH REPRODUCTIVE FAILURE IN THE STATE OF SÃO PAULO, BRAZIL

ABSTRACT

Porcine Parvovirus infection is a common cause of porcine reproductive failure that is mainly represented by embryonic and fetal death, in the absence of any other clinicai signs. The present paper describes the first PPV isolation in the state of São Paulo, which was achieved through infection of culture cell lines of swine kidney (SK-6) by organ suspensions of naturally infected fetuses. Lung, liver and kidneys of PPV infected fetuses were ground and suspended as a 20% suspension in phosphate buffered saline. After these were frozen and thawed three times and cleared by centrifugation at 2,000g for 20 minutes, the Hemaglutination Activity (HA) using 1% suspension of guinea pig erytrocytes was measured and the cell lines was infected by the supematants. The infected cells were frozen and thawed three times, 72 hours post-infection and the supematants were cleared by centrifugatiori at 800g. After a sample had been taken for the HA test, several passages using the sarne method were made for each isolate until a consistent HA activity (titer 32) was detected in the cell culture supematant. Specificity of HA was proved by inhibition with PPV antiserum. The PPV isolation from pig herds confirms the presence of such vírus in the state of São Paulo.

KEY WORDS:
Porcine parvovirus; porcine parvovirosis; isolation; diagnostic