ABSTRACT
The use of different substances for the storage of the entomopathogenic nematodes Steinernemacarpocapsae A11 and Heterorhabditis sp. JPM4 was evaluated. The nematodes were kept in distilled water (control) and the other treatments were made with water with the addition of: Tween 80® (0.1%), ethylene glycol (0.1%), glycerin (1%), glucose (1%), CaCO3 (0.1%), Triton® (0.1%), KMnO4 (0.01%) and NaOCl (0.1%). The evaluations were made at 30, 60, 90, 120, 150 and 180 days, counting the number of infective juveniles (IJ) and determining their survival and infectivity. It was found that glycerin acted as a preserving substance at the temperature of 28º C for both nematodes and also in 16º C for S. carpocapsae A11. The other substances tested, even when they kept the nematodes alive, did not show this effect in relation to infectivity.
KEY WORDS
Heterorhabditis
;
Steinernema
; biological control; persistence; survival
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