Demonstration of antibody and cellular immune response to brain extract in West and Lennox-Gastaut syndromes

Mota, Norma G. S.; Rezkallah-Iwasso, M. Thereza; Peraçoli, M. Terezinha S.; Montelli, Terezinha C. B.

doi:10.1590/S0004-282X1984000200004

Abstracts

We investigated humoral and cellular immune response to brain tissues in 15 patients with West syndrome, in 9 patients with Lennox-Gastaut syndrome and in 20 healthy children. High levels of a precipitating antibody to a saline extract of brain tissue were detected in all patients; leucocyte migration inhibition test with the same antigen was found to be positive in most of them. The role of this autoimmune response in the pathogenesis of West and Lennox-Gastaut syndromes remains to the elucidated.

Investigamos a resposta imunológica celular e humoral frente a extrato salino de tecido cerebral em 9 pacientes com síndrome de Lennox-Gastaut, 15 pacientes com síndrome de West e 20 crianças normais. A técnica de imunodifusão dupla em gel de agar (Ouchterlony) evidenciou em todos os pacientes, altos níveis de um anticorpo precipitante contra o extrato salino de tecido cerebral. O teste de inibição de migração de leucócitos com o mesmo antígeno mostrou-se positivo na maioria dos pacientes. O possível papel destas respostas autoimmunes na patogenia das sindromes de West e Lennox-Gastaut é discutido.

Demonstration of antibody and cellular immune response to brain extract in West and Lennox-Gastaut syndromes

Demonstração de anticorpos e resposta imune celular a extrato de cérebro nas sindromes de West e Lennox-Gastaut

Norma G. S. Mota^I; M. Thereza Rezkallah-Iwasso^I; M. Terezinha S. Peraçoli^I; Terezinha C. B. Montelli^II

^IDepartment of Microbiology and Immunology, IBBMA - UNESP

^IIDepartment of Neurology, IBBMA - UNESP

SUMMARY

We investigated humoral and cellular immune response to brain tissues in 15 patients with West syndrome, in 9 patients with Lennox-Gastaut syndrome and in 20 healthy children. High levels of a precipitating antibody to a saline extract of brain tissue were detected in all patients; leucocyte migration inhibition test with the same antigen was found to be positive in most of them. The role of this autoimmune response in the pathogenesis of West and Lennox-Gastaut syndromes remains to the elucidated.

RESUMO

Investigamos a resposta imunológica celular e humoral frente a extrato salino de tecido cerebral em 9 pacientes com síndrome de Lennox-Gastaut, 15 pacientes com síndrome de West e 20 crianças normais. A técnica de imunodifusão dupla em gel de agar (Ouchterlony) evidenciou em todos os pacientes, altos níveis de um anticorpo precipitante contra o extrato salino de tecido cerebral. O teste de inibição de migração de leucócitos com o mesmo antígeno mostrou-se positivo na maioria dos pacientes. O possível papel destas respostas autoimmunes na patogenia das sindromes de West e Lennox-Gastaut é discutido.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Acknowledgements - The authors wish to thank Dr. Florence Kerr Correa for manuscript revision and Mr. Irineu Machado for technical assistance.

Departamento de Microbiologia e Imunologia, IBBMA, UNESP - 18610 Rubião Junior, Botucatu, SP - Brasil.

1. BOWEN, F. P.; KOSAROVA, J.; CASELLA, D.; NICKLAS, W. & BERL, S. - Focal epileptogenic activity induced by topical application of antisera to brain actomyosin-like protein. Brain. Res. 102:363, 1976.
2. CANTOR, H.; McVAY-BROUDREAU, L.; HUNGENBERG, J.; NAIDORF, K.; SHEN, F. W. & GERSHON, R. K. - Immunoregulatory circuits among T cell sets. II. Physiologic role of feedback inhibition in vivo: absence in NZB mice. J. exp. Med. 147:1116, 1978.
3. CHEVRIE, J. J. - Syndrome de West. Encicl. Méd. Chir. Neurologie, 17045 A80:5. Paris, 1979.
4. ETTLINGER, G. & LOWRIE, M. B. - An immunological factor in epilepsy. Lancet 1:1386, 1976.
5. GORDON, N. - Long-term prognosis after infantile spasms. Develop. Med. Child. Neurol. 23:260, 1981.
6. GRABAR, P. - Hypothesis: auto-antibodies and immunological theories: an analytical review. Clin. Immunol. Immunopathol. 4:453, 1975.
7. HANDWEGER, B. S.; FERNANDES, G. & BROWN, D. M. - Immune and autoimune aspects of diabetes mellitus. Human Pathol. 11:338, 1980.
8. JERNE, N. K. - The immune system. Sci. amer. 229:52, 1973.
9. KAPP, J. A.; PIERCE, C. W.; THEZE, J. & BENACERRAF, B. - Modulation of immune response by supressor T cells. Fed. Proc. 37:2361, 1978.
10. KARPIAK, S. E.; GRAF, L. & RAPPORT, M. M. - Antiserum to brain gangliosides produces recurrent epileptiform activity. Science 194:735, 1976.
11. KARPIAK, S. E.; SEROKOSZ, M. & RAPPORT, M. M. - Effects of antisera to S-100 protein and to synaptic membrane fraction on mice performance and EEG. Brain Res. 102:313, 1976.
12. LANDO, Z.; TEITELBAUM, D. & ARNON, R. - Effect of cyclophosphamide on supressor cell activity in mice unresponsive to EAE. J. Immunol. 123:2156, 1979.
13. LOWRY, O. H.; ROSENBROUGH, N. J.; FARR, A. L. & RANDALL, R. J. - Protein measurement with the phenol reagent. J. biol. Chem. 193:265, 1951.
14. MONTELLI, T. C. B.; IWASSO, M. T. R.; PERAÇOLI, M. T. S. & MOTA, N. G. S. - Cell mediated and humoral immunity in West Syndrome. Arq. Neuro-Psiquiat. (São Paulo) 39:1, 1981.
15. MOTA, N. G. S.; KIT, Y.; REZKALLAH-IWASSO, M. T. & PERAÇOLI, M. T. S. - Humoral and cell-mediated immunity in large non-toxic multidodular goitre. Clin. Endocrinol. 13:173, 1980.
16. OUCHTERLONY, O. - Antigen-antibody reaction in gels. Acta pathol. microbiol. scand. 29:507, 1949.
17. REES, A. J. - Autoimmunity and autoimmune disease. Brit. J. Anaesthesiol. 51: 13, 1979.
18. REES, A. J.; LOCKWOOD, C. M. & PETERS, D. K. - Nephritis due to antibodies to glomerular basement membrane. In: P. Kincoid-Smith & A. J. F. d'Apice (eds.) - Progress in glomerulonephritis. John Wiley, New York, 1978.
19. REINSKOV, T. - Demonstration of precipitating antibody to extract of brain tissue in patients with hypsarrhytmia. Acta Paediat. suppl. 140:73, 1963.
20. SHOENFELD, Y.; PICK, A. I.; DANZIER, Y.; KALACZI, I.; FROLICHMAN, R. & PINKHAS, J. - Immunoglobulin changes in SLE. Ann. Allergy 39:99, 1977.
21. TALAL, N.; ROUBINIAN, J. R.; SHEAR, H.; HOM, J. T. & MIYASAKA, N. - Progress in the mechanims of autoimmune disease. Prog. Immunol. IV :889, 1980.
22. TOURTELOTTE, W. W.; BAUNMHEFNER R. W.; POTVIN, A. R.; MA, B. I.; POTVIN, J. H.; MENDEZ, M. & SYNDULKO, K. - Multiple sclerosis de novo CNS IgG synthesis: effect of ACTH and corticosteroids. Neurology (Minneapolis) 30:1155, 1980.

Publication Dates

Publication in this collection
13 Aug 2012
Date of issue
June 1984

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BOWEN, F. P.; KOSAROVA, J.; CASELLA, D.; NICKLAS, W. & BERL, S. - Focal epileptogenic activity induced by topical application of antisera to brain actomyosin-like protein. Brain. Res. 102:363, 1976.

[2] 2. CANTOR, H.; McVAY-BROUDREAU, L.; HUNGENBERG, J.; NAIDORF, K.; SHEN, F. W. & GERSHON, R. K. - Immunoregulatory circuits among T cell sets. II. Physiologic role of feedback inhibition in vivo: absence in NZB mice. J. exp. Med. 147:1116, 1978.

[3] 3. CHEVRIE, J. J. - Syndrome de West. Encicl. Méd. Chir. Neurologie, 17045 A80:5. Paris, 1979.

[4] 4. ETTLINGER, G. & LOWRIE, M. B. - An immunological factor in epilepsy. Lancet 1:1386, 1976.

[5] 5. GORDON, N. - Long-term prognosis after infantile spasms. Develop. Med. Child. Neurol. 23:260, 1981.

[6] 6. GRABAR, P. - Hypothesis: auto-antibodies and immunological theories: an analytical review. Clin. Immunol. Immunopathol. 4:453, 1975.

[7] 7. HANDWEGER, B. S.; FERNANDES, G. & BROWN, D. M. - Immune and autoimune aspects of diabetes mellitus. Human Pathol. 11:338, 1980.

[8] 8. JERNE, N. K. - The immune system. Sci. amer. 229:52, 1973.

[9] 9. KAPP, J. A.; PIERCE, C. W.; THEZE, J. & BENACERRAF, B. - Modulation of immune response by supressor T cells. Fed. Proc. 37:2361, 1978.

[10] 10. KARPIAK, S. E.; GRAF, L. & RAPPORT, M. M. - Antiserum to brain gangliosides produces recurrent epileptiform activity. Science 194:735, 1976.

[11] 11. KARPIAK, S. E.; SEROKOSZ, M. & RAPPORT, M. M. - Effects of antisera to S-100 protein and to synaptic membrane fraction on mice performance and EEG. Brain Res. 102:313, 1976.

[12] 12. LANDO, Z.; TEITELBAUM, D. & ARNON, R. - Effect of cyclophosphamide on supressor cell activity in mice unresponsive to EAE. J. Immunol. 123:2156, 1979.

[13] 13. LOWRY, O. H.; ROSENBROUGH, N. J.; FARR, A. L. & RANDALL, R. J. - Protein measurement with the phenol reagent. J. biol. Chem. 193:265, 1951.

[14] 14. MONTELLI, T. C. B.; IWASSO, M. T. R.; PERAÇOLI, M. T. S. & MOTA, N. G. S. - Cell mediated and humoral immunity in West Syndrome. Arq. Neuro-Psiquiat. (São Paulo) 39:1, 1981.

[15] 15. MOTA, N. G. S.; KIT, Y.; REZKALLAH-IWASSO, M. T. & PERAÇOLI, M. T. S. - Humoral and cell-mediated immunity in large non-toxic multidodular goitre. Clin. Endocrinol. 13:173, 1980.

[16] 16. OUCHTERLONY, O. - Antigen-antibody reaction in gels. Acta pathol. microbiol. scand. 29:507, 1949.

[17] 17. REES, A. J. - Autoimmunity and autoimmune disease. Brit. J. Anaesthesiol. 51: 13, 1979.

[18] 18. REES, A. J.; LOCKWOOD, C. M. & PETERS, D. K. - Nephritis due to antibodies to glomerular basement membrane. In: P. Kincoid-Smith & A. J. F. d'Apice (eds.) - Progress in glomerulonephritis. John Wiley, New York, 1978.

[19] 19. REINSKOV, T. - Demonstration of precipitating antibody to extract of brain tissue in patients with hypsarrhytmia. Acta Paediat. suppl. 140:73, 1963.

[20] 20. SHOENFELD, Y.; PICK, A. I.; DANZIER, Y.; KALACZI, I.; FROLICHMAN, R. & PINKHAS, J. - Immunoglobulin changes in SLE. Ann. Allergy 39:99, 1977.

[21] 21. TALAL, N.; ROUBINIAN, J. R.; SHEAR, H.; HOM, J. T. & MIYASAKA, N. - Progress in the mechanims of autoimmune disease. Prog. Immunol. IV :889, 1980.

[22] 22. TOURTELOTTE, W. W.; BAUNMHEFNER R. W.; POTVIN, A. R.; MA, B. I.; POTVIN, J. H.; MENDEZ, M. & SYNDULKO, K. - Multiple sclerosis de novo CNS IgG synthesis: effect of ACTH and corticosteroids. Neurology (Minneapolis) 30:1155, 1980.