Eletroforese em acetato de celulose das proteínas do líquido cefalorraqueano: valores normais

Caldas Filho, Izelino; Campos, Gilberto Belisário; Pittella, Carlos F. Homem

doi:10.1590/S0004-282X1977000300008

Resumos

Foi feita eletroforese das proteinas de LCR colhido por via lombar de 19 pacientes neurologicamente normais entre 10 e 54 anos. A proteinorraquia total variou entre 10 e 28 mg% (método de Meulemans). Sete pacientes eram normais e 12 apresentavam neuroses. As médias das frações separadas pela eletroforese foram: pré-albumina 4.3%; albumina 54.8%; globulina alfa-1 5.9%; globulina alfa-2 8.3%; globulinas beta 15.5%; globulinas gama 11.2%.

Determinating the total protein contents by the Meulemans method and using strips of celulose acetate "Cellogel" for electrophoresis the authors have studied the CSF of 19 patients between 10 and 54 years old. Total proteins was found between 10 and 28 mg%. The proteins were separated in six fractions with the following results: pré-albumin 4.3%; albumin 54.8%; a1-globulin 5.9%; a2, globulin 8.3%; b-globulins 15.57c and d-giobulins 11.2%.

Eletroforese em acetato de celulose das proteínas do líquido cefalorraqueano: valores normais

Electrophoresis of cerebrospinal fluid proteins: normal values

Izelino Caldas Filho^I; Gilberto Belisário Campos^II; Carlos F. Homem Pittella^III

^IEx-residente. Serviço de Neurologia do Hospital das Clínicas da Faculdade de Medicina da UFMG

^IIChefe do Departamento de Neurologia e Psiquiatria. Hospital das Clínicas da Faculdade de Medicina da UFMG

^IIIChefe do Serviço de Patologia Clínica da Fundação Benjamim Guimarães, Belo Horizonte MG

RESUMO

Foi feita eletroforese das proteinas de LCR colhido por via lombar de 19 pacientes neurologicamente normais entre 10 e 54 anos. A proteinorraquia total variou entre 10 e 28 mg% (método de Meulemans). Sete pacientes eram normais e 12 apresentavam neuroses. As médias das frações separadas pela eletroforese foram: pré-albumina 4.3%; albumina 54.8%; globulina alfa-1 5.9%; globulina alfa-2 8.3%; globulinas beta 15.5%; globulinas gama 11.2%.

SUMMARY

Determinating the total protein contents by the Meulemans method and using strips of celulose acetate "Cellogel" for electrophoresis the authors have studied the CSF of 19 patients between 10 and 54 years old. Total proteins was found between 10 and 28 mg%. The proteins were separated in six fractions with the following results: pré-albumin 4.3%; albumin 54.8%; a₁-globulin 5.9%; a₂, globulin 8.3%; b-globulins 15.57c and d-giobulins 11.2%.

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1. ABRAMOWICZ, M. - Normal values for CSF protein concentration in children. Clin. Ped. 8:300, 1969.
2. BELANGER, C. & MARTIN, J. L. - Études sur le liquide cephalo-rachidien par l'électrophorèse sur papier: I. Méthodes et valeurs narmales. Laval Méd. 26:622. 1958.
3. BOOIJ, J. - Electrophoresis in CSF proteins. Folia Psychiat. neerl. 52:247. 1949.
4. BOOIJ, J. - Electrophoresis in CSF proteins. Folia Psychiat. neerl. 53:502, 1950.
5. BOOIJ, J. - Electrophoresis in CSF proteins (gama-globulins in particular). Folia Psychiat. neerl. 55:1, 1952.
6. BURROWS, S. - A simple method for concentration of CSF for protein electrophoresis. Clin. Chem. 2:937, 1965.
7. CASTELLO, C. J. - La patologia de las proteinas del líquido cefalorraquídeo: su studio mediante la electroforesis sobre gel de agar-agar. Arch. Neurobiol. (Madrid) 32:655. 1969.
8. CERVOS-NAVARRO, J. & MATIAR, J. - Zur Frage der Intrathekalen Regulation und Genese der Liquorproteine. Dtsch. Z. Nervenheilk. 176:614, 1969.
9. CLAUSEN, J. - Proteins in normal cerebrospinal fluid not found in serum. Proc. Soc. Exp, Biol. Med. 107:170, 1961.
10. ESSER, H. - Die elektrophoretische Untersuchung der Liquoreiweisskörper und ihre klinische Bedeutung. Münch, med. Wschr. 94:2313, 1952.
11. HANZAL, F. & VYMAZAL, J. - The significance of electrophoretic investigation in inflamatory diseases of the nervous system. Acta Psychiat. Neurol. Scand. 33:283. 1958.
12. HORDYNSKY, W. E. - Importance of pre-albumin, alpha 1, alpha 2 and tau fractions in cerebrospinal fluid. Amer. J. Clin. Pathol. 57:251, 1972.
13. KABAT, E. A.; LANDOW, H. & MOORE, D. H. - CSF electrophoresis. Proc. Soc. exp. Biol. 49:260, 1942.
14. KABAT, E. A.; MOORE, D. H. & LANDOW, H. - Electrophoretic study of proteins in CSF and their relationship to the serum proteins. J. Clin. Inv. 21:571, 1942.
15. KAPLAN, A. & JOHNSTONE, M. - Concentration of CSF proteins and their fractionation by cellulose acetate electrophoresis. Clin. Chem. 12:717, 1966.
16. KAPLAN, A. - Electrophoresis of cerebrospinal fluid proteins. Am. J. Med. Sciences. 253:549, 1967.
17. LOWENTHAL, A. - Agar Gel Electrophoresis in Neurology. Elsevier Publishing Co., New York. 1964.
18. PENNOCK, C. A.; PASSANT L. P. & BOLTON, F. G. - Estimation of cerebrospinal fluid protein. J. Clin. Path. 21:518, 1968.
19. SCHEID, K. F. & SCHEID, L. - Elektrophoretische Trennung der Eiweisskorper in Liquor cerebrospinalis. Arch. Psychiat. Nervenk. 117:219, 312 e 641, 1944.
20. SCHULLER, E. - The corrent state over research on CSF protein. Ann. Biol. Clin. (Paris) 30:297, 1972.
21. SHERWIN, R. S. & MOORE, G. H. -- Microzone electrophoresis of unconcentrated cerebrospinal fluid using cellulose acetate strips and nigrosin dye. Amer. J. Clin. Path. 55:705, 1971.
22. SCHÖNENBERG, H. - Die Papier Elektrophorese der Liquor cerebrospinalis. Ann Paediat. (Basileia) 183:309, 1954.
23. SPINA-FRANÇA, A. - Eletroforese em papel das proteinas do LCR; principais resultados encontrados na literatura. Arq. Neuro-Psiquiat. (São Paulo) 16:223, 1958.
24. SPINA-FRANÇA A. - Eletroforese em papel das proteinas do LCR: valores normais. Arq. Neuro-Psiquiat. (São Paulo) 18:19, 1960.
25. SPINA-FRANÇA, A. - Eletroforese em papel das proteinas do líquido cefalorraqueano. Rev. paul. Med. 59:420, 1961.
26. SPINA-FRANÇA, A.; LUZ, B. R. & HAUSSEN, S. R. -- Proteinograma do líquido cefalorraqueano do recém-nascido normal. Arq. Neuro-Psiquiat. (São Paulo) 32:212, 1974.
27. STARY, Z.; WINTERNITZ, R. & KRAL, A. - Untersuchungen über den Eiweissgehalt der Liquor cerebrospinalis. Z. ges. Neurol. Psychiat. 132:193, 1931.
28. TANAKA, Z. - Electrophoretic study of CSF components: survey in normal liquor. Tohoku J. Exp. Med. (Sendai) 63:245, 1956.
29. TOOLE, J. F. - Special Techniques for Neurologic Diagnosis. F. A. Davis Co. Philadelphia, 1970.
30. YDE, A. - Das Verhältnis zwischen Globulin and Albumin Menge in de Cerebrospinalfluüssigkeit. Acta Psychiat. scand. 9:187. 1934.
31. YDE, A. - Einige Untersuchungen über die Proteinsysteme der Cerebrospinalflüssigkelt. Acta Psychiat. scand. 12:257. 1937.

Datas de Publicação

Publicação nesta coleção
30 Ago 2012
Data do Fascículo
Set 1977

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ABRAMOWICZ, M. - Normal values for CSF protein concentration in children. Clin. Ped. 8:300, 1969.

[2] 2. BELANGER, C. & MARTIN, J. L. - Études sur le liquide cephalo-rachidien par l'électrophorèse sur papier: I. Méthodes et valeurs narmales. Laval Méd. 26:622. 1958.

[3] 3. BOOIJ, J. - Electrophoresis in CSF proteins. Folia Psychiat. neerl. 52:247. 1949.

[4] 4. BOOIJ, J. - Electrophoresis in CSF proteins. Folia Psychiat. neerl. 53:502, 1950.

[5] 5. BOOIJ, J. - Electrophoresis in CSF proteins (gama-globulins in particular). Folia Psychiat. neerl. 55:1, 1952.

[6] 6. BURROWS, S. - A simple method for concentration of CSF for protein electrophoresis. Clin. Chem. 2:937, 1965.

[7] 7. CASTELLO, C. J. - La patologia de las proteinas del líquido cefalorraquídeo: su studio mediante la electroforesis sobre gel de agar-agar. Arch. Neurobiol. (Madrid) 32:655. 1969.

[8] 8. CERVOS-NAVARRO, J. & MATIAR, J. - Zur Frage der Intrathekalen Regulation und Genese der Liquorproteine. Dtsch. Z. Nervenheilk. 176:614, 1969.

[9] 9. CLAUSEN, J. - Proteins in normal cerebrospinal fluid not found in serum. Proc. Soc. Exp, Biol. Med. 107:170, 1961.

[10] 10. ESSER, H. - Die elektrophoretische Untersuchung der Liquoreiweisskörper und ihre klinische Bedeutung. Münch, med. Wschr. 94:2313, 1952.

[11] 11. HANZAL, F. & VYMAZAL, J. - The significance of electrophoretic investigation in inflamatory diseases of the nervous system. Acta Psychiat. Neurol. Scand. 33:283. 1958.

[12] 12. HORDYNSKY, W. E. - Importance of pre-albumin, alpha 1, alpha 2 and tau fractions in cerebrospinal fluid. Amer. J. Clin. Pathol. 57:251, 1972.

[13] 13. KABAT, E. A.; LANDOW, H. & MOORE, D. H. - CSF electrophoresis. Proc. Soc. exp. Biol. 49:260, 1942.

[14] 14. KABAT, E. A.; MOORE, D. H. & LANDOW, H. - Electrophoretic study of proteins in CSF and their relationship to the serum proteins. J. Clin. Inv. 21:571, 1942.

[15] 15. KAPLAN, A. & JOHNSTONE, M. - Concentration of CSF proteins and their fractionation by cellulose acetate electrophoresis. Clin. Chem. 12:717, 1966.

[16] 16. KAPLAN, A. - Electrophoresis of cerebrospinal fluid proteins. Am. J. Med. Sciences. 253:549, 1967.

[17] 17. LOWENTHAL, A. - Agar Gel Electrophoresis in Neurology. Elsevier Publishing Co., New York. 1964.

[18] 18. PENNOCK, C. A.; PASSANT L. P. & BOLTON, F. G. - Estimation of cerebrospinal fluid protein. J. Clin. Path. 21:518, 1968.

[19] 19. SCHEID, K. F. & SCHEID, L. - Elektrophoretische Trennung der Eiweisskorper in Liquor cerebrospinalis. Arch. Psychiat. Nervenk. 117:219, 312 e 641, 1944.

[20] 20. SCHULLER, E. - The corrent state over research on CSF protein. Ann. Biol. Clin. (Paris) 30:297, 1972.

[21] 21. SHERWIN, R. S. & MOORE, G. H. -- Microzone electrophoresis of unconcentrated cerebrospinal fluid using cellulose acetate strips and nigrosin dye. Amer. J. Clin. Path. 55:705, 1971.

[22] 22. SCHÖNENBERG, H. - Die Papier Elektrophorese der Liquor cerebrospinalis. Ann Paediat. (Basileia) 183:309, 1954.

[23] 23. SPINA-FRANÇA, A. - Eletroforese em papel das proteinas do LCR; principais resultados encontrados na literatura. Arq. Neuro-Psiquiat. (São Paulo) 16:223, 1958.

[24] 24. SPINA-FRANÇA A. - Eletroforese em papel das proteinas do LCR: valores normais. Arq. Neuro-Psiquiat. (São Paulo) 18:19, 1960.

[25] 25. SPINA-FRANÇA, A. - Eletroforese em papel das proteinas do líquido cefalorraqueano. Rev. paul. Med. 59:420, 1961.

[26] 26. SPINA-FRANÇA, A.; LUZ, B. R. & HAUSSEN, S. R. -- Proteinograma do líquido cefalorraqueano do recém-nascido normal. Arq. Neuro-Psiquiat. (São Paulo) 32:212, 1974.

[27] 27. STARY, Z.; WINTERNITZ, R. & KRAL, A. - Untersuchungen über den Eiweissgehalt der Liquor cerebrospinalis. Z. ges. Neurol. Psychiat. 132:193, 1931.

[28] 28. TANAKA, Z. - Electrophoretic study of CSF components: survey in normal liquor. Tohoku J. Exp. Med. (Sendai) 63:245, 1956.

[29] 29. TOOLE, J. F. - Special Techniques for Neurologic Diagnosis. F. A. Davis Co. Philadelphia, 1970.

[30] 30. YDE, A. - Das Verhältnis zwischen Globulin and Albumin Menge in de Cerebrospinalfluüssigkeit. Acta Psychiat. scand. 9:187. 1934.

[31] 31. YDE, A. - Einige Untersuchungen über die Proteinsysteme der Cerebrospinalflüssigkelt. Acta Psychiat. scand. 12:257. 1937.