Transcription analysis of TIMP-1 and NM23 genes in glioma cell invasion

Santos, José Augusto Nasser dos

doi:10.1590/S0004-282X2005000500037

THESES

Transcription analysis of TIMP-1 and NM23 genes in glioma cell invasion (Abstract)^* * Análise transcricional dos genes TIMP-1 e NM23 na invasão celular em astrocitoma difuso e glioblastoma multiforme (Resumo). Tese de Doutorado. Universidade Federal de São Paulo, EPM/UNIFESP (Área: Ciências, Neurocirurgia). Orientador: Fernando Patriani Ferraz. ** Address: Avenida Ataúlfo de Paiva 1079/1001, 22440-031 Rio de Janeiro RJ, Brasil. E-mail: nasser@riodejaneiro.net . Thesis. São Paulo, 2005

José Augusto Nasser dos Santos^** * Análise transcricional dos genes TIMP-1 e NM23 na invasão celular em astrocitoma difuso e glioblastoma multiforme (Resumo). Tese de Doutorado. Universidade Federal de São Paulo, EPM/UNIFESP (Área: Ciências, Neurocirurgia). Orientador: Fernando Patriani Ferraz. ** Address: Avenida Ataúlfo de Paiva 1079/1001, 22440-031 Rio de Janeiro RJ, Brasil. E-mail: nasser@riodejaneiro.net

PURPOSE: To evaluate using transcription analysis the presence and importance of two genes: NM23-H1 and TIMP-1 on control of tumor cell invasion in diffuse astrocytomas (WHO II) and glioblastoma multiforme (WHO IV).

METHOD: Northern Blot analysis of NM23-H1 and TIMP-1 was performed. Eight diffuse astrocytomas and nineteen glioblastomas (WHO IV) were analyzed to determine if TIMP-1 and NM23-H1 were candidates to inhibition of tumor cell invasion quantitated RNA levels. The samples were collected directly from operating room. Total cellular RNA was extracted from frozen tissue samples using guanidinium-isothiocyanate and cesium chloride gradients. Total RNA (10ml per sample) from tumor tissue were size fractionated through 1% agarose-formaldehyde gels and transferred to nylon filters and then hybridized to ³²P-labeled DNA probes and placed for autoradiography. Levels of specific RNAs were determined by computer-assisted laser densitometry. Blot filters were sequentially hybridized to nm23 and TIMP-1 probes in addition to GAPDH, as a control. Statistical analyses were carried out according to t-test for equality of means.

RESULTS: NM23-H1 was detected in each sample, however it was not correlate malignancy and invasiveness with NM23-H1 expression. On the other side TIMP-1 gene expression showed a clear correlation between low expression and invasiveness.

CONCLUSION: The data suggest that TIMP-1 is an inhibitor of high grade gliomas invasion. NM23-H1 was present in the entire gliomas sample, but it did not vary in diffuse astrocytomas and glioblastomas.

Key words: tumor cell invasion, TIMP-1, NM23, RNA levels, gliomas.

*

Análise transcricional dos genes TIMP-1 e NM23 na invasão celular em astrocitoma difuso e glioblastoma multiforme (Resumo). Tese de Doutorado. Universidade Federal de São Paulo, EPM/UNIFESP (Área: Ciências, Neurocirurgia). Orientador: Fernando Patriani Ferraz.

**

Address: Avenida Ataúlfo de Paiva 1079/1001, 22440-031 Rio de Janeiro RJ, Brasil. E-mail:

nasser@riodejaneiro.net

Publication Dates

Publication in this collection
02 Mar 2006
Date of issue
Sept 2005

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