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A histological study of human allografts

Due to the increasing use of the human allografts in the reconstructive orthopedic surgeries, the complete knowledge of its biomechanics and hystologics characteristic is needed. This study compared allografs picked of femoral condilar from cool air cadavers and after cryopreservation (under -80°C) for thirty days with relationship to the hystologic´s characteristic. Twenty specimens were compared in each group, with relationship to the following hystologic's parameters: cellular viability, vascularization presence, necrosis, and maintenance of the bony matrix, inflammatory process, bony remodelation and fibrosis. After statistical analysis using the Fisher's method(p<0,05), the study concluded that only the cellular viability had significant change after the cryopreservation. The bony grafts don't need viable cells for being used, so the cryopreservation process is an useful method for the allografts´s tissue bank storage, not making unfeasible its future employment in orthopedic surgeries.

Allograft; Histology; Cryopreservation


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