Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4

Krüger, Ernesto Renato; Penha, Tânia Regina; Hummelgen, Fernanda Cristina; Agottani, Jorge Bacila; Reva, Demétrio; Gonçalves, Robson; Thomaz-Soccol, Vanete

doi:10.1590/S1516-89132015050187

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Human and Animal Health • Braz. arch. biol. technol. 58 (5) • Sep-Oct 2015 • https://doi.org/10.1590/S1516-89132015050187 copy

Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.

Key words:
BoHV-4; serology; diagnostic; cattle; epidemiology

ELISA absorbance		RESULTS
BoHV-4	BoHVI-1	BoHV-4	BoHV-1
0.193	1.009	N	P
0.146	0.517	N	P
0.41	0.092	P	N
0.011	0.458	N	P
0.129	0.919	N	P
0.202	1.014	GZ	P
0.095	0.882	N	P
0.3	1.048	GZ	P
0.011	1.136	N	P
0.05	0.760	N	P
0.078	1.268	N	P
-0.05	0.514	N	P
0.41	1.014	P	P
0.089	0.434	N	P
0.033	0.980	N	P
-0.078	0.892	N	P
-0.011	0.848	N	P
-0.05	0.863	N	P
0.707	1.312	P	P
0.092	0.98	N	P
0.308	2.089	GZ	P
-0.033	0.936	N	P
0.09	0.834	N	P
0.085	0.88	N	P
-0.007	1.022	N	P
-0.051	1.147	N	P

Comparisons		Frequency
Positive	concordance	2
Positive	discordance	20
Negative	concordance	1
Negative	discordance	0
Indexes	% global concordance	8.70%
Kappa	-9.03%

Brazil State	Frequency		Z-Test		p		Observations
Brazil State	P	N	GR	P	N	GR	P	N	GR	P	N	GR
MG	157	156	129	9.21	-12.77	6.48	0.0000	0.0000	0.0000	o>e	o<e	o>e
GO	77	227	66	0.32	0.13	-0.49	0.7515	0.8939	0.6222	o=e	o=e	o=e
PR	38	133	40	-0.85	0.63	0.09	0.3948	0.5296	0.9280	o=e	o=e	o=e
SC	36	168	37	-2.19	2.96	-1.44	0.0283	0.0031	0.1490	o<e	o>e	o=e
BA	13	40	11	0.02	0.24	-0.32	0.9852	0.8086	0.7475	o=e	o=e	o=e
SP	12	56	14	-1.29	1.38	-0.39	0.0987	0.1686	0.6940	o=e	o=e	o=e
RO	11	89	17	-3.01	3.44	-1.20	0.0026	0.0006	0.2287	o<e	o>e	o=e
RJ	7	8	2	2.16	-1.19	-0.74	0.0307	0.2346	0.4596	o>e	o=e	o=e
AM	6	3	0	3.48	-1.71	-1.44	0.0005	0.0874	0.1488	o>e	o=e	o=e
MS	3	52	10	-3.19	3.19	-0.70	0.0014	0.0014	0.4815	o<e	o>e	o=e
RS	3	116	7	-5.17	7.40	-3.93	0.0000	0.0000	0.0001	o<e	o>e	o<e
DF	2	8	2	-0.31	0.40	-0.18	0.7584	0.6889	0.8542	o=e	o=e	o=e
MT	1	49	4	-3.41	4.54	-2.17	0.0006	0.0000	0.0304	o<e	o>e	o<e
Total	366	1105	339

Instituto de Tecnologia do Paraná - Tecpar Rua Prof. Algacyr Munhoz Mader, 3775 - CIC, 81350-010 Curitiba PR Brazil, Tel.: +55 41 3316-3052/3054, Fax: +55 41 3346-2872 - Curitiba - PR - Brazil
E-mail: babt@tecpar.br

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