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Articles • Braz. arch. biol. technol. 42 (3) • 1999 • https://doi.org/10.1590/S1516-89131999000300006 linkcopy

Open-access Chimeric ß-glucosidase between Cellvibrio gilvus and Ruminococcus albus enzymes

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    Abstracts

    Based on homology search in the amino acid sequences, a chimeric ß-glucosidase with enzyme activity has been prepared by replacing C-terminal region of Thr509-Arg646 from Cellvibrio gilvus with N-terminal region of Arg160-Gly283 from Ruminococcus albus. The chimeric enzyme showed broader optimum pH and slightly higher heat stability compared with original C. gilvus enzyme.

    Chimeric; Cellvibrio gilvus; Ruminococcus albus; ß-glucosidase

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