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Engineering, Technology and Techniques • Braz. arch. biol. technol. 60 • 2017 • https://doi.org/10.1590/1678-4324-2017160260   copy

Enhancement of biohydrogen production from starch processing wastewater and further inside its ecosystem disclosed by 16S rDNA sequencing and FISH

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ABSTRACT

Biohydrogen production from starch processing wastewater in this study resulted the highest yield of 61.75 mL H2/g COD at initial pH 7.0, thermophilic temperature, and iron concentration 800 mg Fe/L. The yield was 2-folded higher than the operation at mesophilic temperature or without iron addition. Cell immobilization by addition of biomaterials (BM) could improve the hydrogen yield by 2-folded comparing to the non-addition. BM from plants (loofa sponge) was found producing higher yield than that from animals (silk cocoon), and optimal concentration of BM was 5% (V/V). Furthermore, it was revealed further inside its ecosystem using SEM, 16S rDNA sequencing and FISH. There was found rod-shaped microorganisms of Bacillus cereus, which reported as efficient starch-utilizing hydrogen producers, was dominant in the system with population of 47% of all specie identified.

Key words:
biohydrogen production; starch processing wastewater; biological supporting materials; Bacillus cereus

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