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Agriculture, Agribusiness and Biotechnology • Braz. arch. biol. technol. 49 (4) • July 2006 • https://doi.org/10.1590/S1516-89132006000500003 linkcopy

Open-access Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system

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    Abstracts

    Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.

    Amylases; aqueous two-phase systems; Bacillus subtilis; partition; poly (ethylene glycol)

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    Instituto de Tecnologia do Paraná - Tecpar Rua Prof. Algacyr Munhoz Mader, 3775 - CIC, 81350-010 , Tel: +55 41 3316-3054 - Curitiba - PR - Brazil
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