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Bioprocess and Biotechnology • Braz. arch. biol. technol. 48 (spe) • June 2005 • https://doi.org/10.1590/S1516-89132005000400014 linkcopy

Open-access Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography

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    Abstracts

    This paper describes a method for the plasmid DNA purification, which includes an ammonium sulphate precipitation, followed by hydrophobic interaction chromatography (HIC) using Phenyl Sepharose 6 Fast Flow (low sub). The use of HIC took advantage of the more hydrophobic character of single stranded nucleic acid impurities as compared with double-stranded plasmid DNA.

    Gene therapy; hydrophobic interaction chromatography; plasmid; purification

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