This is a preliminary study undertaken to investigate the composition and distribution of amphipods with reference to depth, salinity and sediment at Enseada da Fortaleza (Area 1) and parts of Rio Claro and Rio Escuro (Area 2), Ubatuba, São Paulo State. Eighteen specif of gammarids occurred in the region, of which fourteen known species had been recorded previously for the Brazilian coast. Some of these species as Atylus minikoi and Corophium sp were found at the two areas, while others were recorded at only one of the areas.