Perfil proteômico e metabólico de <i>Piper tuberculatum</i> (Piperaceae)

Cotinguiba, F.; López, S. N.; Budzinski, I. G. F.; Labate, C. A.; Kato, M. J.; Furlan, M.

doi:10.1590/1519-6984.07816

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Brazilian Journal of Biology

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Sumário

Original Article • Braz. J. Biol. 78 (1) • Fev 2018 • https://doi.org/10.1590/1519-6984.07816 copiar

Perfil proteômico e metabólico de Piper tuberculatum (Piperaceae)

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumo

Piper tuberculatum (Piperaceae) é uma espécie que acumula especialmente amidas como metabólitos secundários e diversas atividades biológicas dessa espécie foram relatadas anteriormente. No presente artigo, relatamos um estudo proteômico dessa espécie. Eletroforese bidimensional (2D SDS-PAGE) e espectrometria de massas (ESI-Q-TOF) foram utilizadas nesse estudos. Mais de cem spots e vários peptídeos foram identificados nesta espécie e as funções putativas desses peptídeos relacionadas a mecanismo de defesa como estresse biótico e abiótico foram atribuídos. As informações apresentadas ampliam a gama de informações moleculares dessa espécie.

Palavras-chave:
Piperaceae; amides; Piper tuberculatum; proteômica; 2D SDS-PAGE

Spot	Protein Score	Total Ion Score	Peptide Sequences	ID (NCBI)	Proteins	Species	Mr (kDa) and pI (Iit.)	Mr (kDa) and pI (exp.)	Coverage (%)	Access	Biological Process
*P. tuberculatum (leaves)*
6	63	63	GGLDFTKDDENVNSQPFMR	gi\|15148424	RuBisCO	Cardiandra alternifolia	52416/ 6.08	71480/ 6.32	6	Q0INY7	1.1.1.2
7	58	58	DTDILAAFR	gi\|8096541	RuBisCO (large subunit)	Solanum lycopersicum	53398/ 6.55	54825/ 3.89	6	Q0INY7	1.1.1.2
8	177	117	DITLGFVDLLRDDFIEQDR	gi\|169563	RuBisCO (large subunit)	Solanum tuberosum	53371/ 6.19	51615/ 4.97	11	Q0INY7	1.1.1.2
11	111	79	VALEACVKAR	gi\|169563	RuBisCO (large subunit)	Solanum tuberosum	53371/ 6.19	52677/ 6.65	11	Q0INY7	1.1.1.2
12B	141	78	VALEACVKAR	gi\|349144	RuBisCO (large subunit)	Solanum lycopersicum	53398/ 6.55	52744/ 6.92	12	Q0INY7	1.1.1.2
13	55	55	VALEACVKAR	gi\|1352794	RuBisCO (large subunit)	Harpagophytum grandidieri	52139/ 6.23	46612/ 5.33	6	Q0INY7	1.1.1.2
17	279	96	IVDSFPGQSIDFFGALR	gi\|10720247	RuBisCO (large subunit)	Oryza sativa	50897/ 8.61	43219/ 5.74	12	Q0INY7	1.1.1.2
18	131	105	IVDSFPGQSIDFFGALR	gi\|10720247	RuBisCO (large subunit)	Oryza sativa	48055/ 5.85	43226/ 5.91	6	Q0INY7	1.1.1.2
9	341	124	IFNVLGEPVDNLGPVDTR	gi\|52082860	ATPase (subunit β)	Piper hispidum	50620/ 5.04	51980/ 5.26	20	P11574	1.1.3
30	193	124	VAVLGAAGGIGQPLALLMK	gi\|126896	malate dehydrogenase	Citrullus lanatus	36406/ 8.88	36711/ 7.40	10	P17606	1.1.3
29	60	60	ILFIGGTGYIGK	gi\|1708422	isoflavone reductase	Solanum tuberosum	33831/ 6.16	32566/ 5.92	3	Q00016	1.2.8
14	191	135	AAQDIALAELAPTHPIR	gi\|1345588	Protein 14-3-3-like	Saccharum (hybrid cultivar CP65-357)	28978/ 4.79	46447/ 5.52	22	Q96299	2.1.2
37	185	135	AAQDIALAELAPTHPIR	gi\|1345588	Protein 14-3-3-like	Sugar cane	28978/ 4.79	28619/ 5.81	16	Q96299	2.1.2
38	50	50	ADVDNKESVGK	gi\|1345588	RNA polymerase IV (second large unit)	Rhododendron macrophyllum	116144/ 8.98	27455/ 5.71	1	Q9LK40	2.1.2
27	39	39	ELGIGIVPYSPIGR	gi\|78708648	Protein induction auxin	Oryza sativa	37905/ 5.81	37698/ 5.76	4	Q9ZTX8	2.2.3
28	152	72	SGPLQPGVDIIEGPVK	gi\|132270	Rubber elongation factor	Hevea brasiliensis	14713/ 5.04	36441/ 6.10	42	P15252	2.2.3
46	82	58	SGPLQPGVDIIEGPVK	gi\|132270	Rubber elongation factor	Hevea brasiliensis	14713/ 5.04	18613/	19	P15252	2.2.3
4	41	41	VIDEIRER	gi\|3763925	Ligation protein/ clathrin	Arabidopsis thaliana	30781/ 9.30	60320/ 5.1	2	Q8VY07	3.3
3	224	111	SLDIERPTYTNLNR	gi\|6094431	α-3-tubulin	Hordeum vulgare	50193/ 4.95	62256/ 4.90	11	P08841	4.3
45	54	54	IWHHTFYNELR	gi\|3219759	Actin	Glycine max	41796/ 5.23	21742/ 5.32	2	Q96292	4.3
44	45	45	LSGEVDANKAEVK	gi\|159487461	Protein 2 - chromosome structural maintenance	Chlamydomonas reinhardtii	128165/ 6.66	23389/ 4.92	1	Q9C5Y4	4.5
47	222	119	LPTDETLVAQIK	gi\|162458009	Factor 5A - Initiation of eukaryotic translation	Zea mays	17714/ 5.61	19100/ 4.63	34	Q76E23	5.3.3
1	97	45	AVVTVPAYFNDSQR	gi\|24638460	heat shock protein 70	Cucumis sativus	75480/ 5.15	76250/ 4.81	4	P22953	5.3.5
5	61	61	GVEELADAVR		GroEL-like chaperone	Vitis vinifera	61673/ 5.85	61293/ 5.52	1	Q8L7B5	5.3.5
10	441	130	KIEDAVDSAISWLDSNQLAEVDEFEDK	gi\|242054931	Hypothetical protein SORBIDRAFT_03g039360	Sorghum bicolor	71261/ 5.10	52456/ 6.15	27		6.2
P. tuberculatum (fruits)
S7	137	83	ELAAQPDVDGFLVGGASLKPEFIDIINAATVK	gi\|195605636	Triose phosphate isomerase	Zea mays	27278/ 5.53	35125/ 5.32	28%	P48491	1.1.2
S2	190	91	TVLIMELINNIAK	gi\|6687280	ATP synthase (β subunit)	Codonopsis pilosula	53628/ 5.07	57186/ 5.10	15%	P11574	1.1.3
S4	46	46	LKLELAQFAELQAFAQFASALDK	gi\|51556908	ATP synthase (α subunit)	Elymus sibiricus	55549/ 6.03	55566/ 6.12	4%	P11574	1.1.3
S6	404	148	DNLTLWTSDISEDPAEEIR	gi\|195635799	Protein 14-3-3-like (GF14-6)	Zea mays	30985/ 4.84	42255/ 7.82	20%	Q96299	2.1.2
S9	205	114	SSITPGTVLILLAGR	gi\|195605226	60S ribosomal protein L6	Zea mays	24273/ 10.14	30158/ 5.42	11%	Q9C9C5	4.4.2
S10	127	127	AMVPVAEVNVPVVGGHAGITILPLFSQATPK	gi\|224061310	Not identified	Populus trichocarpa	35704/ 8.53	35732/ 7.38	9%	-	6.3

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