CONTINUOUS AND SEMICONTINUOUS REACTION SYSTEMS FOR HIGH-SOLIDS ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSICS

Quiroga, A. González; Silvera, A. Bula; Padilla, R. Vasquez; Costa, A. C. da; Maciel Filho, R.

doi:10.1590/0104-6632.20150324s00003547

Acessibilidade / Reportar erro

Brasil

Brazilian Journal of Chemical Engineering

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Bioprocess Engineering • Braz. J. Chem. Eng. 32 (4) • Oct-Dec 2015 • https://doi.org/10.1590/0104-6632.20150324s00003547 copy

CONTINUOUS AND SEMICONTINUOUS REACTION SYSTEMS FOR HIGH-SOLIDS ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSICS

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

An attractive operation strategy for the enzymatic hydrolysis of lignocellulosics results from dividing the process into three stages with complementary goals: continuous enzyme adsorption at low-solids loading (5% w/w) with recycling of the liquid phase; continuous liquefaction at high-solids content (up to 20% w/w); and, finally, continuous or semicontinuous hydrolysis with supplementation of fresh enzymes. This paper presents a detailed modeling and simulation framework for the aforementioned operation strategies. The limiting micromixing situations of macrofluid and microfluid are used to predict conversions. The adsorption and liquefaction stages are modeled as a continuous stirred tank and a plug flow reactor, respectively. Two alternatives for the third stage are studied: a train of five cascading stirred tanks and a battery of batch reactors in parallel. Simulation results show that glucose concentrations greater than 100 g L^-1 could be reached with both of the alternatives for the third stage.

Keywords
Reactor; High-solids; Continuous; Semicontinuous; Micromixing; Recycling

PROCESS	REACTORS CONFIGURATION	REF
SHF	PFR followed by a train of three to twelve cascading CSTRs PFR followed by two trains of three to twelve cascading CSTRs Two PFR in parallel followed by a train of three to twelve cascading CSTRs	Harlick and Zheng, 2011Harlick, P. J. and Zheng, W., Process for improving the hydrolysis of cellulose in high consistency systems. U.S. Patent Application, 13/216,368 (2011).
SHF	A train of three cascading CSTRs A train of three cascading CSTRs with liquid phase recycling from the third to the first reactor, enzyme supplementation at the second reactor and liquid phase withdrawing at the outlet of the first reactor A train of three cascading CSTRs with distributed feeding of enzyme and distributed withdrawing of liquid phase	*Sjoede et al., 2013*Sjoede, A., Froelander, A., Lersch, M., Roedsrud, G., Hals, K. and Kloeften, A., Lignocellulosic biomass conversion. U.S. Patent No. 20,130,217,074 (2013).
SHF	A train of ten cascading CSTRs or a PFR, both with and without recycle A train of ten cascading CSTRs with distributed feeding of substrate and enzyme at the first two or three reactors A train of three cascading CSTRs with distributed feeding of substrate and enzyme at the first two or three reactors, followed by a PFR	Gonzalez Quiroga, 2009Gonzalez Quiroga, A., Modelagem, simulação e analise de reatores contínuos para a hidrólise enzimática de bagaço de cana. MSc Thesis, Universidade Estadual de Campinas, Brazil (2009). (In Portuguese).; *Gonzalez Quiroga et al.,2010aGonzalez Quiroga, A., Costa, A. and Maciel Filho, R., Analysis of conversion and operation strategies for enzymatic hydrolysis of lignocellulosic biomass in a series of CSTRs with distributed feeding. Bioprocess Biosyst Eng, 33, 901-910 (2010a)., 2010b*Gonzalez Quiroga, A., Costa, A. and Maciel Filho, R., Modelamiento y simulación de una serie de CSTR's con alimentación distribuida para la hidrólisis enzimática de bagazo de caña. Revista ION, 23(1), 13-19 (2010b).
SSF	Three trains of six cascading CSTRs	*Wooley et al.,* 1999**Wooley, R., Ruth, M., Sheehan, J., Ibsen, K., Majdeski, H. and Galvez A., Lignocellulosic biomass to ethanol process design and economics utilizing cocurrent dilute acid prehydrolysis and enzymatic hyrolysis current and futuristic scenarios. No. NREL/TP-580-26157 (1999).
SHF	Train of five cascading CSTRs	*Aden et al.,* 2002**Aden, A., Ruth, M., Ibsen, K., Jechura, J., Neeves, K., Sheehan, J. and Wallace, B., Lignocellulosic biomass to ethanol process design and economics utilizing co-current dilute acid prehydrolysis and enzymatic hydrolysis for corn stover. No. NREL/ TP-510-32438 (2002).
SHF	Tower type PFR (downward flow) followed by twelve batch in parallel	*Humbird et al.,* 2011**Humbird, D., Davis, R. and Tao, L., Process design and economics for biochemical conversion of lignocellulosic biomass to ethanol. Nº NREL/TP5100-47764 (2011).
SSF	Train of four STRs with intermittent feeding	*Shao et al.,* 2009**Shao, X., Lynd, L., Wyman, C. and Bakker, A., Kinetic modeling of cellulosic biomass to ethanol via simultaneous saccharification and fermentation: Part I. Accommodation of intermittent feeding and analysis of staged reactors. Biotechnol. Bioeng., 102(1), 59-65 (2009).

Operation procedure		Yield %	Sugars concentration [g L^-1]
1)	5% w/w solids and all the enzymes added at the beginning	64	26
2)	20% w/w solids and all the enzymes added at the beginning	44	84
3)	All substrate and cellulase added at the beginning. Slurry thickened to 20% w/w after 10 min. Xylanase and BG supplemented after 8 h	59	114
4)	All substrate and part of the cellulase added at the beginning. Slurry thickened to 20% w/w after 10 min. Cellulase, xylanase and BG supplemented after 2 h	63	121

Brazilian Society of Chemical Engineering Rua Líbero Badaró, 152 , 11. and., 01008-903 São Paulo SP Brazil, Tel.: +55 11 3107-8747, Fax.: +55 11 3104-4649, Fax: +55 11 3104-4649 - São Paulo - SP - Brazil
E-mail: rgiudici@usp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *To whom correspondence should be addressed