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Effect of pre-gelatinized temperature on physical and nutritional content of indonesian instant cassava leaves porridge: rowe luwa

Efeito da temperatura de pré-gelatinizada no conteúdo físico e nutricional do mingau instantâneo de folhas de mandioca: rowe luwa, típico da Indonésia

Abstract

Rowe luwa is a traditional porridge from Southwest Sumba, Indonesia, made from the steamed pulp cassava leaves pounded together with rice. This study examined the effect of pre-gelatinization temperature on the physical quality and nutritional content of instant rowe luwa porridge. The experimental design used in this study was a Completely Randomized Design (CRD) with pre-gelatinization temperature factors, viz., 60 °C (X1), 65 °C (X2), 70 °C (X3), 75 °C (X4) and 80 °C (X5). Physical properties were measured by analyzing color, viscosity, rehydration, and syneresis, while for nutritional compositions, water content, ash, fat, protein, carbohydrate, and energy were evaluated. The simple linear regression was employed to examine the correlation between pre-gelatinization temperature and physical properties or nutritional composition. The results showed that the pre-gelatinization temperature correlated with physical properties, such as: viscosity (r = 0.9924), rehydration (r = 0.807) and syneresis (r = 0.841). Furthermore, the pre-gelatinized temperature significantly affected protein and carbohydrate contents (p < 0.05), while the moisture, ash, and fat contents showed negligible effect (p > 0.05). Principal Component Analysis (PCA) showed that instant rowe luwa porridge prepared with the pre-gelatinization temperature of 80 °C (object X5) had the highest viscosity, rehydration, syneresis, and protein contents compared to the other cooking temperatures. These preliminary data are useful for further research to determine the method and optimization formula of the instant rowe luwa porridge.

Keywords:
Drum dryer; Indonesian cassava leaves porridge; Pre-gelatinization

Resumo

Rowe luwa é um mingau tradicional do sudoeste de Sumba, na Indonésia, feito de polpa cozida no vapor de folhas de mandioca triturada junto com arroz. Neste estudo, o efeito da temperatura de pré-gelatinização sobre a qualidade física e o conteúdo nutricional do mingau instantâneo de rowe luwa foi examinado. O desenho experimental utilizado neste estudo foi um desenho completamente aleatório (CRD) com fatores de temperatura de pré-gelatinização, viz., 60 °C (X1), 65 °C (X2), 70 °C (X3), 75 °C ( X4) e 80 °C (X5). As propriedades físicas foram medidas através da análise de cor, viscosidade, reidratação e sinérese, enquanto que, para as composições nutricionais, foram avaliados os teores de água, cinzas, gordura, proteína, carboidrato e valor calórico. A regressão linear simples foi empregada para examinar a correlação entre a temperatura de pré-gelatinização e as propriedades físicas ou a composição nutricional. Os resultados mostraram que a temperatura de pré-gelatinização apresenta correlação com propriedades físicas, tais como: viscosidade (r = 0,9924), reidratação (r = 0,807) e sinérese (r = 0,841). Além disso, a temperatura de pré-gelatinização afetou significativamente os teores de proteínas e carboidratos (p< 0,05), enquanto que, para os teores de umidade, cinzas e gordura, apresentou efeito desprezível (p > 0,05). A análise de componentes principais (PCA) mostrou que o mingau instantâneo de rowe luwa com a temperatura de pré-gelatinização de 80 °C (objeto X5) apresentou os maiores valores de viscosidade, reidratação, sinérese e conteúdo de proteína, em comparação com as outras temperaturas de cozimento estudadas. Estes dados preliminares são úteis para pesquisas futuras para determinar o método de preparação e a fórmulação otimizada do mingau instantâneo de rowe luwa.

Palavras-chave:
Secador de tambor; Mingau de folhas de mandioca indonésio; Pré-gelatinização

1 Introduction

Malnutrition, stunting, and over nutrition are nutritional problems worldwide, especially in poor and developing countries (United Nations International Children's Emergency Fund, 2020United Nations International Children's Emergency Fund – UNICEF. World Health Organization – WHO.(2020). Malnutrition prevalence remains alarming: stunting is declining too slowly while wasting still impacts the lives of far too many young children. Retrieved in 2021, March 8, from https://data.unicef.org/topic/nutrition/malnutrition/
https://data.unicef.org/topic/nutrition/...
). According to the World Health Organization (WHO), Indonesia is among the third countries with the highest prevalence of stunting in Southeast Asia. Based on the basic health research data in 2018, it showed that the prevalence of stunting in the Southwest Sumba regency, East Nusa Tenggara reached 61.2%, exceeding the national stunting condition (Ministry of Health of Indonesia, 2019Ministry of Health of Indonesia. (2019). Riset Kesehatan Dasar (Riskesdas). Jakarta: Ministry of Health of Indonesia.). The data indicate that the increasing of stunting prevalence in this regency should be a concern of Indonesian government to intervene in a stunting management scheme.

One of the local wisdom foods from Southwest Sumba is rowe luwa porridge. Rowe luwa porridge comes from rowe word, which means leaf, and luwa, which means cassava. Rowe luwa is a green pulp made from cassava leaves pounded together with rice. It can be developed for local food innovation. Moreover, it is expected to provide various foods based on local bio-resources, to support stunting management. Besides, the manufacture of instant rowe luwa aims to extend its product shelf life. Furthermore, drying is a good alternative for its conservation.

Looking at current market trend, consumers prefer instant products or ready to eat products compared to conventional ones. Statistical data on the consumption of instant porridge in Indonesia showed that each year, consumption rate of instant porridge in Indonesia has increased of 31.47% (2011-2015) (Biro Pusat Statistik, 2016Biro Pusat Statistik – BPS. (2016). Consumption expenditure of population of indonesia. Jakarta: Central Bureau of Statistics.). Instant food, usually in dry or concentrate form, makes it practice to consume and can be served in a short time. According to Mayachiew et al. (2015)Mayachiew, P., Charunuch, C., & Devahastin, S. (2015). Physicochemical and thermal properties of extruded instant functional rice porridge powder as affected by the addition of soybean or mung bean. Journal of Food Science, 80(12), E2782-E2791. PMid:26523755. http://dx.doi.org/10.1111/1750-3841.13118
http://dx.doi.org/10.1111/1750-3841.1311...
, some important criteria in instant porridge production are hydrophilic properties, not having an impermeable gel layer, and rehydration of the final product, which does not produce clumping and settling products.

Formulation development is a critical stage in product manufacturing because it will affect to the final product. The mixing process of ingredients in the formulation of rowe luwa porridge will affect the resulting product. According to Makame et al. (2019)Makame, J., Cronje, T., Emmambux, N. M., & De Kock, H. (2019). Dynamic oral texture properties of selected indigenous complementary porridges used in African communities. Foods, 8(6), 221. PMid:31234403. http://dx.doi.org/10.3390/foods8060221
http://dx.doi.org/10.3390/foods8060221...
, the viscosity of instant porridge that has been brewed varied from slightly thick to thick; this is due to the greater concentration of white rice flour addition, the higher rice flour addition, the higher of thickness of the instant porridge. Drum drying is a promising alternative to instantization fruit flakes (Nunes et al., 2020Nunes, L. P., Ferrari, C. C., Ito, D., Souza, E. C. G., & Germer, S. P. M. (2020). Drum drying process of jabuticaba pulp using corn starch as an additive. Brazilian Journal of Food Technology, 23, e2019166. http://dx.doi.org/10.1590/1981-6723.16619
http://dx.doi.org/10.1590/1981-6723.1661...
).

In instance-based process, pre-gelatinization process is a modification of starch, made through a process involving water and heat to break down all or part of the granules, then dried to produce complete and partial starch pre-gelatinization (Wadchararat et al., 2006Wadchararat, C., Masubon, T., & Naivikul, O. (2006). Characterization of pregelatinized and heat moisture treated rice flours. Witthayasan Kasetsat Witthayasat, 40, 144-153.; Palguna et al., 2014Palguna, I. G. P. A., Sugiyono, S., & Hariyanto, B. (2014). Karakteristik pati sagu yang dimodifikasi dengan perlakuan gelatinisasi dan retrogradasi berulang. Jurnal Pangan, 23(6), 146-156.). Several factors influence this pre-gelatinization process. Among of them is temperature. Therefore, this study aimed to examine the effect of pre-gelatinization temperature on the physical quality and nutritional content of instant rowe luwa porridge.

2 Materials and methods

This research was conducted in June 2020 - April 2021. The research took place at the Indonesian Institute of Sciences (LIPI), Research Center for Appropriate Technology, Subang District, West Java-Indonesia (-6.554421141915637, 107.76218022289622) and it will be implemented in the Southwest Sumba Regency, NTT-Indonesia.

2.1 Materials

Fresh cassava (Manihot esculenta Crantz) leaves, rice flour, shallots, garlic, salt, salam leaves, lemongrass, ginger, sugar, and coconut milk, were purchased in a traditional market, Subang, Indonesia. Sucrose, n-hexane, CuSO4.5H2O, H2SO4, (NH4)2HPO4, HCl, Na2CO3, KIO3, and NaOH were obtained from Sigma-Aldrich, Singapore. Citric acid, distillated water, starch, phenolphthalein 1%, LuffSchoorl’s solution and Pb-acetate were taken from the chemical stockroom at Research Center for Appropriate Technology, Indonesian Institute of Sciences (LIPI). All reagents were analysis grade.

2.2 Preparation of samples

Young cassava leaves, ± 4-6 months, trimmed to cut the leaves and stalks. Cassava leaves (10.59%), rice flour (5.30%), bay leaves (0.34%), shallots (1.01%), garlic (0.67%), lemongrass (0.84%), ginger (0.84%), salt (0.34%), sugar (0.41%), coconut milk (5.04%), and water (74.61%) were weighed. Cassava leaves and rice flour were crushed using a chopper (Philips HD 3115, China). Then, the ingredients were mixed and cooked with cooking temperatures of 60 ºC (X1), 65 ºC (X2), 70 ºC (X3), 75 ºC (X4), and 80 ºC (X5) for 40 minutes. The slurry dough was then dried using a drum dryer at temperature of 115-120 °C, with a rotating speed of 8 Hz. The dry rowe luwa porridge was then mashed using a chopper and sieved to a size of 60 mesh. Finally, instant rowe luwa porridge was packaged and ready to be analyzed.

2.3 Procedure of analyses

2.3.1 Viscosity measurement

A Rapid Visco Analyser (RVA-Techmaster, Macquarie Park, Australia), interfaced with a personal computer equipped was used to measure the viscosity of instant rowe luwa porridge (Amagloh et al., 2013Amagloh, F. K., Mutukumira, A. N., Brough, L., Weber, J. L., Hardacre, A., & Coad, J. (2013). Carbohydrate composition, viscosity, solubility, and sensory acceptance of sweetpotato- and maize-based complementary foods. Food & Nutrition Research, 57(1), 18717. PMid:23516115. http://dx.doi.org/10.3402/fnr.v57i0.18717
http://dx.doi.org/10.3402/fnr.v57i0.1871...
).The samples were weighed 3 g and then mixed with 25 mL of distilled water in an aluminum container. The sample was then put into the Rapid Visco Analyzer with a rotation speed of 100 rpm at 25 °C for 2 minute. The sample was then heated to 95 °C in 5 minutes, then held at 95 °C for 3 minutes. After that, the samples were again cooled to 50 °C in 4 minutes and then held at 50 °C for 2 minutes.

2.3.2 Color analysis

The color of rowe luwa instant porridge samples were observed using high-quality colourimeter NH 310 (Indriati et al., 2020Indriati, A., Hidayat, D. D., Andriansyah, R. C. E., Iwansyah, A. C., & Surahman, D. N. (2020). Characterisation of physical, mechanical and colour properties of Muntingia calabura fruits. In S. K. Wahono (Ed.), IOP Conference Series: Earth and Environmental Science: Vol. 462, No. 1. Tangerang: IOP Publishing. http://dx.doi.org/10.1088/1755-1315/462/1/012044
http://dx.doi.org/10.1088/1755-1315/462/...
). The analysis methods used were CIE (Commission Internationale de L'Eclairage) L* a* b* and hue coordinates. Coordinate L* represents the clarity, in which L = 0 is black, and L* = 100 is colourless. Coordinate a* represents the shade of red and green, in which a* > 0 indicates red colour and a*< 0 means green colour. Coordinate b* represents the tone of blue and yellow, in which b* > 0 shows the intensity of yellow and b* < 0 indicates the hue of blue. The hue (h*) is the characteristics of the colour, i.e. red, yellow, green, and blue.

2.3.3 Rehydration

To determine the rehydration of instant rowe luwa porridge, an amount of 1 g of sample instan rowe luwa porridge was weighed and then soaked in hot water. The rehydration was carried out at 95 °C (10 min) in water bath filled with distilled water. Then, centrifugation (300 rpm) was carried out for 25 minutes. Each sample was filtered from the water, and weighed. The final moisture content of instant rowe luwa porridge after reconstitution was determined according to AOAC standards (Zielinska & Markowski, 2012Zielinska, M., & Markowski, M. (2012). Color characteristics of carrots: effect of drying and rehydration. International Journal of Food Properties, 15(2), 450-466. http://dx.doi.org/10.1080/10942912.2010.489209
http://dx.doi.org/10.1080/10942912.2010....
). Each measurement was carried out in triplicate.

2.3.4 Syneresis

Rowe luwa porridge suspension (5%, w/w) was heated at 90 °C for 30 min in a temperature-controlled water bath, followed by rapid cooling in an ice water bath to room temperature. Then, the sample was stored for 24 hours at 4 °C. Syneresis (%) was measured as amount of water released after centrifugation at 3200 X g for 15 minutes (Singh et al., 2006Singh, N., Kaur, L., Sandhu, K. S., Kaur, J., & Nishinari, K. (2006). Relationships between physicochemical, morphological, thermal, rheological properties of rice starches. Food Hydrocolloids, 20(4), 532-542. http://dx.doi.org/10.1016/j.foodhyd.2005.05.003
http://dx.doi.org/10.1016/j.foodhyd.2005...
; Li et al., 2016Li, H., Prakash, S., Nicholson, T. M., Fitzgerald, M. A., & Gilbert, R. G. (2016). Instrumental measurement of cooked rice texture by dynamic rheological testing and its relation to the fine structure of rice starch. Carbohydrate Polymers, 146, 253-263. PMid:27112873. http://dx.doi.org/10.1016/j.carbpol.2016.03.045
http://dx.doi.org/10.1016/j.carbpol.2016...
).

2.3.5 Nutritional composition

Nutritional compositions viz., protein, moisture, fat, ash, carbohydrate of instant rowe luwa porridge were determined (Association of Official Agricultural Chemists, 2004Association of Official Agricultural Chemists – AOAC. (2004). Official methods of analysis (15th ed., Vol. 1). Arlington: AOAC Inc. https://doi.org/10.1016/b0-12-765490-9/00006-9
https://doi.org/10.1016/b0-12-765490-9/0...
). Moisture and ash contents were determined by using the gravimetric method (Association of Official Agricultural Chemists, 2004Association of Official Agricultural Chemists – AOAC. (2004). Official methods of analysis (15th ed., Vol. 1). Arlington: AOAC Inc. https://doi.org/10.1016/b0-12-765490-9/00006-9
https://doi.org/10.1016/b0-12-765490-9/0...
). The Buchi-Dumaster equipment was used to measure the protein content (Association of Official Agricultural Chemists, 2004Association of Official Agricultural Chemists – AOAC. (2004). Official methods of analysis (15th ed., Vol. 1). Arlington: AOAC Inc. https://doi.org/10.1016/b0-12-765490-9/00006-9
https://doi.org/10.1016/b0-12-765490-9/0...
), while fat was measured by the Weibull method (Association of Official Agricultural Chemists, 2004Association of Official Agricultural Chemists – AOAC. (2004). Official methods of analysis (15th ed., Vol. 1). Arlington: AOAC Inc. https://doi.org/10.1016/b0-12-765490-9/00006-9
https://doi.org/10.1016/b0-12-765490-9/0...
), and carbohydrates were calculated (Association of Official Agricultural Chemists, 2004Association of Official Agricultural Chemists – AOAC. (2004). Official methods of analysis (15th ed., Vol. 1). Arlington: AOAC Inc. https://doi.org/10.1016/b0-12-765490-9/00006-9
https://doi.org/10.1016/b0-12-765490-9/0...
). The energy value of instant rowe luwa porridge was calculated by the At-water factor (1 g protein = 4 kcal; 1 g fat = 9 kcal; and 1 g carbohydrate = 4 kcal).

2.4 Statistical analysis

Data were presented as mean ± standard deviation (sd) (n = 3). Normality test was carried out on the data, and Analysis of Variance (ANOVA) was used to determine the significantly differences between treatments. Simple linear regression and principal component analysis (PCA) were used to evaluate the relationship between the pre-gelatinization temperature and the physicochemical properties of instant rowe luwa porridge. Statistical analysis was performed using Microsoft Excel 2013 and XL-STAT Statistical Software.

3 Results and discussion

3.1 Color

The effect of pre-gelatinization temperature on the color of the instant rowe luwa porridge is presented in Table 1. The statistical analysis results showed that the pre-gelatinization temperature significantly affected the *a and *b values ​​of instant rowe luwa porridge (p < 0.05) but had not significantly impact on the *L and hue values (p > 0.05).

Table 1
The results of color analysis of instant rowe luwa porridge based on pre-gelatinization temperature treatment.

The values of *a and *b instant porridge of rowe luwa were 0.77-1.32 and 5.66 to 7.68, respectively (Table1). Generally, the *a and *b values of instant rowe luwa porridge with a pre-gelatinization temperature treatment of 80 °C (X5) were the highest. This findings show that the pre-gelatinization temperature treatment causes a reddish image with a yellowish intensity which is thought to be due to a non-enzymatic browning reaction during the cooking process (pre-gelatinization). These results were agreement with Wijanarka et al. (2017)Wijanarka, A., Sudargo, T., Harmayani, E., & Marsono, Y. (2017). Effect of pre-gelatinization on physicochemical and functional properties of gayam (Inocarfus fagifer forst.) flour. American Journal of Food Technology, 12(3), 178-185. http://dx.doi.org/10.3923/ajft.2017.178.185
http://dx.doi.org/10.3923/ajft.2017.178....
that reported the enzymatic reaction more retarded with the longer pre-gelatinized time of Gayam (Inocarfus fagifer Forst.) flour. Furthermore, the longer pre-gelatinization time also would increase the temperature and deactivate polyphenol oxidase (Akyıldız & Ocal, 2006Akyıldız, A., & Ocal, N. D. (2006). Effects of dehydration temperatures on colour and polyphenoloxidase activity of amasya and golden delicious apple cultivars. Journal of the Science of Food and Agriculture, 86(14), 2363-2368. http://dx.doi.org/10.1002/jsfa.2624
http://dx.doi.org/10.1002/jsfa.2624...
).

3.2 Viscosity

The linear regression graph in viscosity analysis; the correlation coefficient (r) and determination coefficient (R2) are shown in Figure 1. The results obtained showed a relationship between pre-gelatinization temperature and viscosity. These results showed that the value of the correlation coefficient (r) and the coefficient of determination (R2) were 0.9924 and 0.9849, respectively.

Figure 1
Viscosity value of instant rowe luwa porridge based on pregelatinization temperature treatment. Data are presented as mean ± standard error (n=3).

The average viscosity of the instant rowe luwa porridge were 0.165 to 0.667 Pa*s (Figure 1). All pre-gelatinization temperature treatments showed an increasing in viscosity proportional with temperature. The lowest viscosity is at the cooking temperature of 65 °C (0.165 Pa*s), while the highest viscosity is at the cooking temperature of 80 °C (0.667 Pa*s) (p < 0.05). This condition occurred might because the starch contained in the instant rowe luwa porridge have gelatinization due to the influence of cooking temperature. These results were in agreement with Singh et al. (2006)Singh, N., Kaur, L., Sandhu, K. S., Kaur, J., & Nishinari, K. (2006). Relationships between physicochemical, morphological, thermal, rheological properties of rice starches. Food Hydrocolloids, 20(4), 532-542. http://dx.doi.org/10.1016/j.foodhyd.2005.05.003
http://dx.doi.org/10.1016/j.foodhyd.2005...
, which reported that when heated the granules will inflation because they absorb water. Furthermore, it undergoes gelatinization and results in increased viscosity. Muchlisyiyah et al. (2020)Muchlisyiyah, J., Prasmita, H. S., Estiasih, T., & Nurfatimah, R. P. (2020). The effect of pregelatinization with heat and moisture treatment on physicochemical and pasting characteristics of red glutinous rice flour. Research Journal of Life Science, 7(3), 168-176. http://dx.doi.org/10.21776/ub.rjls.2020.007.03.7
http://dx.doi.org/10.21776/ub.rjls.2020....
reported that increase time and temperature have increased gel consistency. Pre-gelatinization has increased the thickness of red glutinous rice flour. Rice flour heated in a longer period has higher viscosity and could be attributed by lower water content (Rohaya et al., 2013Rohaya, M. S., Maskat, M. Y., & Ma’ruf, A. G. (2013). Rheological properties of different degree of pregelatinized rice flour batter. Sains Malaysiana, 42(12), 1707-1714.).

The increase in viscosity is caused by the inflation of starch granules, especially amylose (Donald, 2004Donald, A. M. (2004). Understanding starch structure and functionality. In A. Eliasson (Ed.), Starch in food. Sawston: Woodhead Publishing Limited. http://dx.doi.org/10.1533/9781855739093.1.156
http://dx.doi.org/10.1533/9781855739093....
; Palguna et al., 2014Palguna, I. G. P. A., Sugiyono, S., & Hariyanto, B. (2014). Karakteristik pati sagu yang dimodifikasi dengan perlakuan gelatinisasi dan retrogradasi berulang. Jurnal Pangan, 23(6), 146-156.). According to Sopade et al. (1992)Sopade, P. A., Ajisegiri, E. S., & Badau, M. H. (1992). The use of peleg equation to model water absorption in some cereal grains during soaking. Journal of Food Engineering, 15(4), 269-283. http://dx.doi.org/10.1016/0260-8774(92)90010-4
http://dx.doi.org/10.1016/0260-8774(92)9...
, when the temperature is low, the starch is pregelatinized so that it is easy to absorb air, causing the granules to expand and increase the viscosity. At the gelatinization temperature, the viscosity increase is due to the amylose diffusing the granules to produce a gel. This increase continued until the viscosity peaked, after which the viscosity of the un-gelatinated decreased. It is based on the breakdown of the rice grains and gel structure, with the system turns into a mixture of leached amylose molecules, melted amylopectin sites, and granular fragments (Han & Hamaker, 2001Han, X. Z., & Hamaker, B. R. (2001). Amylopectin fine structure and rice starches paste breakdown. Journal of Cereal Science, 34(3), 279-284. http://dx.doi.org/10.1006/jcrs.2001.0374
http://dx.doi.org/10.1006/jcrs.2001.0374...
; Wijanarka et al., 2017Wijanarka, A., Sudargo, T., Harmayani, E., & Marsono, Y. (2017). Effect of pre-gelatinization on physicochemical and functional properties of gayam (Inocarfus fagifer forst.) flour. American Journal of Food Technology, 12(3), 178-185. http://dx.doi.org/10.3923/ajft.2017.178.185
http://dx.doi.org/10.3923/ajft.2017.178....
).

3.3 Rehydration

The linear regression graph in rehydration analysis; the correlation coefficient (r) and determination coefficient (R2) are shown in Figure 2. Figure 2 showed a relationship between pre-gelatinization temperature and rehydration. These results showed that the value of the correlation coefficient (r) and the coefficient of determination (R2) were 0.9761 and 0.9529, respectively.

Figure 2
Rehydration value of instant rowe luwa porridge based on pregelatinization temperature treatment. Data are presented as mean ± standard error (n=3).

Figure 2 shows that the higher the pre-gelatinization temperature, the more of rehydration value increases. The instant rowe luwa porridge with pre-gelatinization temperature 65 °C (X1) had the lowest rehydration values (639.41%), while the instant rowe luwa porridge with pre-gelatinization temperature 80 °C (X5) had the highest rehydration values (694.72%) (p < 0.05). This increase is probably due to the gelatinization process during the rehydration process. The cell walls will absorb air and soften when the dry material is rehydrated. With elasticity in the cell wall, the cell wall will return to its original shape. These agreed with Awuchi et al. (2019)Awuchi, C.G., Somtochukwu, V., & Kate, C. (2019). The functional properties of foods and flours. International Journal of Advanced Academic Research, 5(11), 139-160. that at 60 °C, the starch granules begin to absorb the liquid and swell. At 80 °C, the granules will have absorbed five times their volume until they burst open, thus releasing starch into the liquid. Gelatinization is completely achieved when the liquid reaches 100 °C. Furthermore, Puspitowati & Driscoll (2007)Puspitowati, S., & Driscoll, R. H. (2007). Effect of degree of gelatinisation on the rheology and rehydration kinetics of instant rice produced by freeze drying. International Journal of Food Properties, 10(3), 445-453. http://dx.doi.org/10.1080/10942910600871289
http://dx.doi.org/10.1080/10942910600871...
that showed the rate of rehydration of rice increases with the degree of gelatinization and the final moisture content. The pre-cooked starch granules swell faster than the ungelatinized rice. The rise of the rehydration rate is like viscosity. Pre-cooked rice viscosity was observed before the gelatinization temperature. The more significant the water absorption of the instant porridge, the more readily it dissolves in the pulp and makes it more accessible during the manufacturing process (Amagloh et al., 2013Amagloh, F. K., Mutukumira, A. N., Brough, L., Weber, J. L., Hardacre, A., & Coad, J. (2013). Carbohydrate composition, viscosity, solubility, and sensory acceptance of sweetpotato- and maize-based complementary foods. Food & Nutrition Research, 57(1), 18717. PMid:23516115. http://dx.doi.org/10.3402/fnr.v57i0.18717
http://dx.doi.org/10.3402/fnr.v57i0.1871...
; Onyango et al., 2020Onyango, C., Luvitaa, S. K., Unbehend, G., & Haase, N. (2020). Nutrient composition, sensory attributes and starch digestibility of cassava porridge modified with hydrothermally-treated finger millet. Journal of Agriculture and Food Research, 2, 100021. http://dx.doi.org/10.1016/j.jafr.2020.100021
http://dx.doi.org/10.1016/j.jafr.2020.10...
).

3.4 Syneresis

Syneresis (%) was defined as the starch pastes freeze-thaw stability, which indicates the percentage of water separated after the starch paste was treated by freezing storage (Haryanti et al., 2014Haryanti, P., Setyawati, R., & Wicaksono, R. (2014). Effect of temperature and time of heating of starch and butanol concentration on the physicochemical. Agritech, 34(3), 308-315. http://dx.doi.org/10.22146/agritech.9459
http://dx.doi.org/10.22146/agritech.9459...
). Syneresis is a separation between starch gel and water (Ariyantoro et al., 2020Ariyantoro, A., Parnanto, N. H., & Kuntatiek, E. (2020). THE effect of various of temperature on physical, chemical and physicochemical properties of yam bean pre-gelatinized flour. Jurnal Teknologi Hasil Pertanian, 8(1), 12-19. http://dx.doi.org/10.20961/jthp.v13i1.40124
http://dx.doi.org/10.20961/jthp.v13i1.40...
). The linear regression graph in rehydration analysis; the correlation coefficient (r) and determination coefficient (R2) are shown in Figure 3.

Figure 3
Syneresis value of instant rowe luwa porridge based on pregelatinization temperature treatment. Data are presented as mean ± standard error (n=3).

Figure 3 shows a relationship between pre-gelatinization temperature and rehydration with values of the correlation coefficient (r) and the coefficient of determination (R2) were 0.841 and 0.9169, respectively. The higher the pre-gelatinization temperature, the higher the syneresis value has increased (p < 0.05). The instant rowe luwa porridge with pre-gelatinization temperature 65 °C (X1) had the lowest syneresis values (35.69%), while the instant rowe luwa porridge with pre-gelatinization temperature 80 °C (X5) had the highest syneresis values (52.11%). This indicates that the X5 treatment produces high-amylose starch, which is less stable to frozen storage than the other treatments. This results was in agreement with Denchai et al. (2019)Denchai, N., Suwannaporn, P., Lin, J., Soontaranon, S., Kiatponglarp, W., & Huang, T. C. (2019). Retrogradation and digestibility of rice starch gels: the joint effect of degree of gelatinization and storage. Journal of Food Science, 84(6), 1400-1410. PMid:31132154., that reported the retrograded rice starch gelatinized at the incomplete gelatinization temperature (77 °C) had the highest percent syneresis than the retrograded rice starch at 95 ᵒC and 121 ᵒC. At the lowest temperature (77 °C), syneresis increased sharply the following temperature. While a little syneresis was found in starch gels gelatinized at higher temperatures (95 °C and 121 °C). The increase in syneresis was contributed to an increase in molecular association between starch chains at reduced temperatures that exclude water from the gel structure (Lan et al., 2017Lan, X., Liu, X., Yang, Y., Wu, J., & Wang, Z. (2017). The effect of lamellar structure ordering on the retrogradation properties of canna starch subjected to thermal and enzymatic degradation. Food Hydrocolloids, 69, 185-192. http://dx.doi.org/10.1016/j.foodhyd.2017.02.004
http://dx.doi.org/10.1016/j.foodhyd.2017...
).

Bhat & Riar (2017)Bhat, F. M., & Riar, C. S. (2017). Studies on effect of temperature and time on textural and rheological properties of starch isolated from traditional rice cultivars of Kashmir (India). Journal of Texture Studies, 48(2), 151-159. PMid:28370109. http://dx.doi.org/10.1111/jtxs.12226
http://dx.doi.org/10.1111/jtxs.12226...
, reported that the process of breaking starch granules due to temperature increases causes amylose molecules to come out of the granules. The higher the temperature, the more amylose molecules that will come out of the starch granules. Syneresis is caused by the retrogradation of amylose (Singh et al., 2006Singh, N., Kaur, L., Sandhu, K. S., Kaur, J., & Nishinari, K. (2006). Relationships between physicochemical, morphological, thermal, rheological properties of rice starches. Food Hydrocolloids, 20(4), 532-542. http://dx.doi.org/10.1016/j.foodhyd.2005.05.003
http://dx.doi.org/10.1016/j.foodhyd.2005...
). The strong bonds between amylose during retrogradation cause more water to separate from the starch gel when the starch gel is put at room temperature. The discharge of large amounts of water during the retrogradation process causes high syneresis (Bhat & Riar, 2017Bhat, F. M., & Riar, C. S. (2017). Studies on effect of temperature and time on textural and rheological properties of starch isolated from traditional rice cultivars of Kashmir (India). Journal of Texture Studies, 48(2), 151-159. PMid:28370109. http://dx.doi.org/10.1111/jtxs.12226
http://dx.doi.org/10.1111/jtxs.12226...
).

3.5 Nutritional composition

Heat treatment can affect micronutrient in foodstuff (Francisquini et al., 2020Francisquini, J. A., Nunes, L., Martins, E., Stephani, R., Perrone, Í. T., & Carvalho, A. F. (2020). How the heat treatment affects the constituents of infant formulas: a review. Brazilian Journal of Food Technology, 23, e2019272. http://dx.doi.org/10.1590/1981-6723.27219
http://dx.doi.org/10.1590/1981-6723.2721...
). Effects of pre-gelatinization temperature to nutritional composition of instant rowe luwa porridge is shown at Table 2. Table 2 shows that the pre-gelatinization temperature treatment of instant rowe luwa porridge had a significant effect on protein and carbohydrate content (p < 0.05), meanwhile, it had no significant impact on moisture, ash, and fat content (p > 0.05). Table 2 shows that the instant rowe luwa porridge with pre-gelatinization temperature 60 °C (X1) and 80 °C (X5) had the highest protein value (16.54%), and the lowest of carbohydrates content (56.58%). The interaction between starch and protein in food systems increased the gel strength, which was attributed to the increase in the density of protein matrix and formation of elastic starch globules (Couto et al., 2012Couto, A., Enes, P., Peres, H., & Oliva-Teles, A. (2012). Temperature and dietary starch level affected protein but not starch digestibility in gilthead sea bream juveniles. Fish Physiology and Biochemistry, 38(3), 595-601. PMid:21728054. http://dx.doi.org/10.1007/s10695-011-9537-5
http://dx.doi.org/10.1007/s10695-011-953...
; Jamilah et al., 2009Jamilah, B., Mohamed, A., Abbas, K. A., Rahman, R. A., Karim, R., & Hashim, D. M. (2009). Protein-starch interaction and their effect on thermal and rheological characteristics of a food system: a review. Journal of Food Agriculture and Environment, 7(2), 169-174.).

Table 2
Nutritional composition of instant rowe luwa porridge based on pregelatinization temperature treatment.

3.6 Principal Component Analysis (PCA)

A principal component analysis was used to classify samples and find variables, viz., proximate composition and physical properties of instant rowe luwa porridge that will contribute to differentiation. Based on the theoretical arguments of PCA as described by (Hair et al., 2005Hair, F., Anderson, J., Tatham, I., & Black, C. (2005). Multivariate data analysis (5th ed.). New Jersey: Prentice Hall.), the significant factor loading values higher than or equal 0.7 were used to identify the most important variables and observations in each dimension, or principal components. The first two factors (F1 and F2) accounted for 46.01% and 21.97%, respectively. The loading factors of F1 had a positive correlation with color of *b value, hue, viscosity, rehydration and syneresis. The strong positive loadings of F2 are protein, fat and energy. According to Jang et al., (2016)Jang, E. H., Lee, S. J., Hong, J. Y., Chung, H. J., Lee, Y. T., Kang, B. S., & Lim, S. T. (2016). Correlation between physicochemical properties of japonica and indica rice starches. Lebensmittel-Wissenschaft + Technologie, 66, 530-537. http://dx.doi.org/10.1016/j.lwt.2015.11.001
http://dx.doi.org/10.1016/j.lwt.2015.11....
, amylose content was positively correlated to pasting temperature, cohesiveness, and protein, but negatively correlated to peak viscosity in japonica and indica rice starches. Figure 4 shows the pregelatinized temperature are differently structured according to their proximate, and physical properties.

Figure 4
Biplot obtained from PCA of variables comprising proximate, and physical properties.

At the pre-gelatinization temperature of instant rowe luwa porridge, the physical properties, viz., viscosity, syneresis and rehydration, have a very close positive relationship. Likewise, the protein and carbohydrates content. The viscosity, syneresis, and rehydration values of instant rowe luwa porridge increase with increased temperature. This is in line with what has been reported by Muchlisyiyah et al. (2020)Muchlisyiyah, J., Prasmita, H. S., Estiasih, T., & Nurfatimah, R. P. (2020). The effect of pregelatinization with heat and moisture treatment on physicochemical and pasting characteristics of red glutinous rice flour. Research Journal of Life Science, 7(3), 168-176. http://dx.doi.org/10.21776/ub.rjls.2020.007.03.7
http://dx.doi.org/10.21776/ub.rjls.2020....
, Puspitowati & Driscoll (2007)Puspitowati, S., & Driscoll, R. H. (2007). Effect of degree of gelatinisation on the rheology and rehydration kinetics of instant rice produced by freeze drying. International Journal of Food Properties, 10(3), 445-453. http://dx.doi.org/10.1080/10942910600871289
http://dx.doi.org/10.1080/10942910600871...
, and Denchai et al. (2019)Denchai, N., Suwannaporn, P., Lin, J., Soontaranon, S., Kiatponglarp, W., & Huang, T. C. (2019). Retrogradation and digestibility of rice starch gels: the joint effect of degree of gelatinization and storage. Journal of Food Science, 84(6), 1400-1410. PMid:31132154. that the level of viscosity, synthesis, and rehydration increased with pre-gelatinization temperature.

Figure 4 shows that instant rowe luwa porridge with a pre-gelatinization temperature of 80 °C (object X5) has highest values ​​of viscosity, rehydration, syneresis, and protein value than other objects. Objects X2 and X3 are objects with similar indicators, with the characteristics of the presentation of carbohydrate values, ash content, and color (a* and b*). Object X3 with the lowest value of all variables. The X1 and X4 object form their own groups.

4 Conclusion

In conclusion, the pre-gelatinization temperature affected physical properties, such as viscosity, rehydration, and syneresis of instant rowe luwa porridge. In nutritional composition, the pre-gelatinized temperature had a significant effect on protein and carbohydrate content, while the moisture, ash, and fat content had no significant effect. The viscosity, syneresis, and rehydration values of instant rowe luwa porridge increase with increased temperature. PCA analysis showed the pregelatinized temperature are differently structured according to their proximate, and physical properties. The instant rowe luwa porridge with a pre-gelatinization temperature of 80 °C (object X5) has the highest value ​​of viscosity, rehydration, syneresis, and protein value than other objects. Objects X2 and X3 were objects with similar indicators, with the characteristics of the presentation of carbohydrate values, ash content, and color. Object X3 with the lowest value of all variables. The X1 and X4 object form their own groups.

Acknowledgements

We are grateful to the Ministry of Financial, Republic of Indonesia for funding the LPDP-Rispro Mandatory-RISTEK/BRIN (No.267/PRN/Stunting/2020), as well as the Indonesian Institute of Sciences (LIPI) for supporting facilities and technical support (ELSA-LIPI). There is no conflict of interest.

  • Cite as: Iwansyah, A. C., Apriadi, T., Arif, D. Z., Andriana, Y., Indriati, A., Mayasti, N. U., & Luthfiyanti, R. (2022). Effect of pre-gelatinized temperature on physical and nutritional content of indonesian instant cassava leaves porridge: rowe luwa. Brazilian Journal of Food Technology, 25, e2021050. https://doi.org/10.1590/1981-6723.05021
  • Funding: Lembaga Ilmu Pengetahuan Indonesia (267/E1/PRN/2020) and Lembaga Pengelola Dana Pendidikan (KEP-32/LPDP/2020)

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Edited by

Section Editor: Mateus Petrarca

Publication Dates

  • Publication in this collection
    25 Feb 2022
  • Date of issue
    2022

History

  • Received
    08 Mar 2021
  • Accepted
    29 Oct 2021
Instituto de Tecnologia de Alimentos - ITAL Av. Brasil, 2880, 13070-178 Campinas - SP / Brasil, Tel 55 19 3743-1762 - Campinas - SP - Brazil
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