5'-Ribonucleotides production using 5'-phosphodiesterase from spent malt roots

Produção de 5'-ribonucleotídeos utilizando 5'-fosfodiesterase da radícula de malte cervejeiro

Abstract

5'-ribonucleotides are high value-added molecules widely used in the food and pharmaceutical industries because of their bioactive properties. The present work aims to produce a composition of 5’-ribonucleotides using spent brewer’s yeast as cheap source of RNA, and barley malt rootlets as cheap source of 5'-phosphodiesterase (5'-PDE). This is a very promising and innovative strategy because both spent yeast and malt rootles are residues of the brewing process and are closely linked in a cycle that until now is not yet commercially exploited due to lack of studies. Our results showed that extraction of 5’-PDE was mainly influenced by the fineness of the rootlets and amount of extraction solvent (water). The main molecules formed during RNA hydrolysis were 5’-ribonucleotides, which represented 85.86% of the total hydrolyzed molecules. Finally, the results of the proposed approach can generate a new perspective for the brewing industry regarding the management of its wastes, generating from them products of high added value and with a wide range of applications.

Keywords:
Brewing industry wastes; Spent brewer’s yeast; Barley malt rootlets; RNA hydrolysis; Enzyme kinetics; Bioprocess optimization