The effect of ozonated water on changes in the quality of blanched edamame during cold storage

Krasnova, Inta; Segliņa, Dalija; Juhņeviča-Radenkova, Karina; Lepse, Līga; Zeipiņa, Solvita

doi:10.1590/1981-6723.14021

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Brazilian Journal of Food Technology

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ORIGINAL ARTICLE • Braz. J. Food Technol. 25 • 2022 • https://doi.org/10.1590/1981-6723.14021 copy

The effect of ozonated water on changes in the quality of blanched edamame during cold storage

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Vegetable soybean (Glycine max (L.) Merr.) is a seasonal vegetable and has limited shelf life. The present study aimed to determine the use of ozonated water as a sanitizer in order to extend the shelf life of blanched edamame and also to study the effect of ozonation on the quality indicators of packaged soybeans. Raw unwashed beans were used as control to test treatments beans washed and blanched in water bath at 93 ± 2 °C for 2 minutes; part of them was cooled in ice cold distilled water and part was cooled in ozonated ice cold distilled water. Chilled edamame was packed in a polypropylene bag, hermetically sealed and stored at the temperature of +4.0 °C for 21 days. The use of ozonated water for cooling the blanched edamame effectively inhibited the development of harmful microorganisms without significant color changes of pods and beans. The data obtained from the analytical hierarchy process revealed that the ozonated water used to cool blanched edamame compared to pure water did not significantly affect the change in phenolic content in 11 days of storage.

Keywords:
Vegetable soybean; Antimicrobial agent; Chemical compounds; Microbiology

HIGHLIGHTS

Highlights The effect of ozonated water for cooling of blanched edamame was studied for the first time.

The ozonated water could prolong the shelf life of blanched edamame and suppress microorganisms.

Phenolic compounds of edamame beans were not detrimentally affected by ozonated water treatment.

Ozonated water did not affect the color of the edamame pod during storage.

Storage time, days	Samples	L*		(-a/b)
Storage time, days	Samples	Pods	Beans	Pods	Beans
1	C	50.87 ± 3.5^ba	54.82 ± 3.9^bc	-0.38 ± 0.04^a	-0.40 ± 0.09^a
	B	51.47 ± 3.0^ab	54.64 ± 2.5^bc	-0.36 ± 0.08^a	-0.39 ± 0.07^a
	BO	45.49 ± 3.2^dc	47.31 ± 3.1^ed	-0.37 ± 0.05^a	-0.39 ± 0.10^a
6	C	56.37 ± 3.1^a	60.29 ± 3.5^a	-0.17 ± 0.04^b	-0.24 ± 0.02^b
	B	47.09 ± 2.9b^c	54.94 ± 2.5b^c	-0.09 ± 0.02^c	-0.30 ± 0.06^ba
	BO	47.20 ± 1.9^c	56.27 ± 3.1b^a	-0.14 ± 0.03^cb	-0.36 ± 0.12^ab
11	C	49.87 ± 2.9^b	53.00 ± 2.8^cb	-0.07 ± 0.01^dc	-0.22 ± 0.13^ba
	B	43.79 ± 2.5^ed	52.35 ± 3.2^cd	-0.10 ± 0.01^c	-0.26 ± 0.10^ba
	BO	45.93 ± 1.7^dc	46.16 ± 3.9^ed	-0.01 ± 0.01^f	-0.26 ± 0.09^b
16	C	54.39 ± 2.8^a	56.44 ± 3.0^ba	-0.03 ± 0.04^ef	-0.16 ±0.10^b
	B	45.16 ± 2.0^dc	50.74 ± 3.3^cd	-0.04 ± 0.01^e	-0.21 ± 0.07^b
	BO	43.91 ± 2.9^ed	48.57 ± 3.1^d	-0.00 ± 0.01^f	-0.24 ± 0.08^b
21	C	46.02 ± 3.0^dc	59.21 ± 3.7^a	-0.03 ± 0.01^e	-0.16 ± 0.09^b
	B	43.68 ± 2.4^ed	53.0 ± 4.2^cb	-0.04 ± 0.02^e	-0.21 ± 0.08^b
	BO	44.57 ± 1.4^d	49.72 ± 3.4^d	-0.00 ± 0.01^f	-0.24 ± 0.09^b

Storage time, days	Samples	Soluble solids, ºBrix	Vitamin C, mg 100 g^-1	Total Phenolic (TP), mg GAE 100 g^-1	Total Tannin (TT), mg GAE 100 g^-1
1	C	12.43 ± 0.2^b	65.61 ±1.1^a	85.10 ± 0.9^a	50.40 ± 0.2^a
	B	12.50 ± 0.1^ba	56.00 ± 1.6^c	78.94 ±1.9^cb	45.30 ± 3.4^cb
	BO	12.52 ± 0.2^ba	52.00 ± 2.1^d	79.11±1.9^cb	43.30 ± 2.0^dc
6	C	12.67 ± 0.2^a	58.42 ± 2.0^b	82.56 ± 2.5^ab	46.21 ± 1.5^c
	B	12.57 ± 0.3^ba	41.64 ± 0.3^f	78.62 ± 1.7^cb	48.23 ± 0.4^b
	BO	12.58 ± 0.3^ab	40.85 ± 1.4^ef	79.02 ± 1.1^c	50.76 ± 1.5^a
11	C	12.71 ± 0.3^ab	48.09 ± 3.3^e	80.52 ± 0.9^bc	26.88 ± 2.4^ef
	B	12.40 ± 0.2^b	32.57 ± 0.2^g	74.05 ± 1.7^e	17.92 ± 1.4^h
	BO	12.42 ± 0.3^ba	25.65 ± 0.4^j	76.39 ± 1.9^d	23.88 ± 2.3^ef
16	C	12.20 ± 0.3^ba	41.04 ± 2.9^f	70.76 ± 1.5^f	17.88 ± 2.4^h
	B	12.13 ± 0.2^cb	27.86 ± 0.2^ih	66.92 ± 1.8^g	10.59 ± 1.2^j
	BO	12.11 ± 0.2^cb	20.89 ± 1.3^k	66.39 ± 1.9^e	20.55 ± 0.2^g
21	C	11.0 ± 0.3^d	30.33 ± 2.6^hg	57.41 ± 4.3^h	17.88 ± 2.4^h
	B	11.70 ± 0.4^c	17.61 ± 0.4^l	45.25 ± 3.1ⁱ	15.26 ± 1.7ⁱ
	BO	11.69 ± 0.5^cd	16.02 ± 0.5^m	44.06 ± 4.1^ji	14.51 ± 1.1^ij

Storage time, days	Samples	Chlorophyll a, mg 100 g^-1	Chlorophyll b, mg 100 g^-1	Total carotenoids, µg 100 g^-1
0	C	33.85 ± 0.7a	17.89 ± 0.2c	3.25 ± 0.1a
	B	22.60 ± 0.9^e	16.50 ± 0.2^d	2.26 ± 0.1^g
	BO	20.80 ± 0.2^f	16.56 ± 0.5^ed	2.74 ± 0.1^e
6	C	26.77 ± 0.9^c	16.32 ± 0.1^ed	3.13 ± 0.1^b
	B	18.50 ± 0.4^h	16.17 ± 0.2^fe	3.00 ± 0.0^c
	BO	17.06 ± 0.7ⁱ	13.95 ± 0.9^h	2.21 ± 0.1^g
11	C	28.90 ± 0.5^b	16.39 ± 0.8^e	2.99 ±0.1^c
	B	15.36 ±0.1^k	22.19 ± 0.2^a	2.97 ± 0.1^c
	BO	19.80 ± 0.6^g	15.00 ± 0.1^g	3.31 ± 0.1^a
16	C	25.46 ± 0.4^d	16.11 ±0.2^f	2.81 ± 0.0^d
	B	15.07 ± 0.2^l	20.07 ± 0.3^b	2.90 ±0.1^c
	BO	16.80 ± 0.2^j	13.60 ± 0.2ⁱ	2.90 ± 0.2^c
21	C	19.63 ± 0.6^g	16.77 ± 0.3^d	2.72 ± 0.1^ed
	B	13.33 ± 0.4ⁿ	16.43 ± 0.4^e	2.37 ± 0.1^f
	BO	13.83 ± 0.4^m	12.80 ± 0.3^j	2.25 ± 0.1^g

Instituto de Tecnologia de Alimentos - ITAL Av. Brasil, 2880, 13070-178 Campinas - SP / Brasil, Tel 55 19 3743-1762 - Campinas - SP - Brazil
E-mail: bjftsec@ital.sp.gov.br

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[1] *Corresponding Author: Inta Krasnova. Institute of Horticulture, Unit Processing and Biochemistryl, Graudu iela 1, Ceriņi, Krimūnu pagasts, Dobeles novads, LV-3701 - Latvia, e-mail: inta.krasnova@llu.lv