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Medical Microbiology • Braz. J. Microbiol. 34 (suppl 1) • Nov 2003 • https://doi.org/10.1590/S1517-83822003000500002 linkcopy

Open-access Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis

Anticorpo anti-intimina: caracterização imunológica e diagnóstico de amostras de EPEC e EHEC

AuthorshipSCIMAGO INSTITUTIONS RANKINGS

    Abstracts

    Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.

    intimin; phenotypical analysis; polyclonal antibody; EPEC; EHEC

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    Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
    E-mail: bjm@sbmicrobiologia.org.br
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