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Food Microbiology • Braz. J. Microbiol. 38 (4) • Dec 2007 • https://doi.org/10.1590/S1517-83822007000400007   copy

Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods

Correlação entre API 20 STREP e PCR multiplex para identificação de Enterococcus spp. isolados de amostras de alimentos no Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

We evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of the isolates.

Enterococcus spp.; identification; API 20 STREP; PCR multiplex; foods

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br
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