Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates

Imunoensaio de captura para detecção da toxina LT produzida por Escherichia coli enterotoxigênica em isolados bacterianos

Abstracts

A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.

detection; polyclonal antibody; monoclonal antibody; thermo-labile toxin; E. coli