Prevalence of the <i>Helicobacter pylori</i> in the tonsils and
                  adenoids

Bayindir, Tuba; Toplu, Yuksel; Otlu, Baris; Yakupogullari, Yusuf; Yildirim, Ozge; Kalcioglu, Mahmut Tayyar

doi:10.1016/j.bjorl.2014.08.018

INTRODUCTION:

There is an ongoing debate about the existence and effects of Helicobacter pylori (Hp) in adenotonsillar tissue.

OBJECTIVE:

A clinical study was conducted to assess the existence of Hp in the adenoid and/or adenotonsillar tissues, which were surgically excised due to chronic adenotonsillitis.

METHODS:

Phosphoglucosamine mutase gene for the detection of Hp and cytotoxin-associated gene as virulence gene were examined in 84 adenotonsillar tissues obtained from 64 patients and patients' serum by using polymerase chain reaction.

RESULTS:

Hp IgG was detected in 57 (89%) patients' serum. A total of seven tissue samples from 64 patients (10.9%) were found positive for Hp DNA, of which five were adenoids and two were tonsil tissues. All polymerase chain reaction positive samples were also positive for the cytotoxin-associated gene, which is a virulence determinant for the organism.

CONCLUSION:

This study suggests that children are exposed to Hp at an early age of their life in this province. Hp may have a role in the pathogenesis of chronic adenotonsillitis, especially in endemic areas.

Helicobacter pylori; Reservoirs; Adenoids; Palatine tonsil

[1] * Corresponding author. E-mail: mtkalcioglu@hotmail.com (M.T. Kalcioglu).

Gene	Primer sequence	PCR product (bp)	PCR conditions	References
glmM	5'-AAGCTTTTAGGGGTGTTAGGGGTTT-3'	294	94 °C, 1 min; 55 °C, 1 min; 72 °C, 1 min (35 cycles)	16
glmM	5'-AAGCTTACTTTCTAACACTAACGC-3'	294	94 °C, 1 min; 55 °C, 1 min; 72 °C, 1 min (35 cycles)	16

cagA	ATAATGCTAAATTAGACAACTTGAGCGA	298	94 °C, 1 min; 60 °C, 1 min; 72 °C, 1 min (45 cycles)	17
cagA	TTAGAATAATCAACAAACATCACGCCA	298	94 °C, 1 min; 60 °C, 1 min; 72 °C, 1 min (45 cycles)	17

Brasil

Brasil

Prevalence of the Helicobacter pylori in the tonsils and adenoids^☆ ☆ Please cite this article as: Bayindir T, Toplu Y, Otlu B, Yakupogullari Y, Yildirim O, Kalcioglu MT. Prevalence of the Helicobacter pylori in the tonsils and adenoids. Braz J Otorhinolaryngol. 2015;81:307-11.

INTRODUCTION:

OBJECTIVE:

METHODS:

RESULTS:

CONCLUSION:

Brasil

Brasil

Prevalence of the Helicobacter pylori in the tonsils and adenoids☆ ☆ Please cite this article as: Bayindir T, Toplu Y, Otlu B, Yakupogullari Y, Yildirim O, Kalcioglu MT. Prevalence of the Helicobacter pylori in the tonsils and adenoids. Braz J Otorhinolaryngol. 2015;81:307-11.

INTRODUCTION:

OBJECTIVE:

METHODS:

RESULTS:

CONCLUSION:

Prevalence of the Helicobacter pylori in the tonsils and adenoids^☆ ☆ Please cite this article as: Bayindir T, Toplu Y, Otlu B, Yakupogullari Y, Yildirim O, Kalcioglu MT. Prevalence of the Helicobacter pylori in the tonsils and adenoids. Braz J Otorhinolaryngol. 2015;81:307-11.