Apoptosis and expression of argyrophilic nucleolus organizer regions in epithelial neoplasms of the larynx

Ribeiro, Christiana Vargas; Vasconcelos, Anilton Cesar; Andrade Filho, José de Souza

doi:10.1016/j.bjorl.2014.12.003

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Brazilian Journal of Otorhinolaryngology

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Artigos Originais • Braz. j. otorhinolaryngol. 81 (2) • Apr 2015 • https://doi.org/10.1016/j.bjorl.2014.12.003 copy

Apoptosis and expression of argyrophilic nucleolus organizer regions in epithelial neoplasms of the larynx ^☆ ☆ Please cite this article as: Ribeiro CV, Vasconcelos AC, Andrade Filho JS. Apoptosis and expression of argyrophilic nucleolus organizer regions in epithelial neoplasms of the larynx. Braz J Otorhinolaryngol. 2015;81:158-66. ^☆☆ ☆☆ Institution: This project was developed at the Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG); and Escola de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. ^☆☆☆ ☆☆☆ Case report: a retrospective study of 40 cases of epithelial neoplasms of the larynx, with 3 degrees of tumor differentiation.

Authorship SCIMAGO INSTITUTIONS RANKINGS

INTRODUCTION:

Occurrence of apoptosis and expression of proliferative markers are powerful tools to establish a prognosis in the follow-up of cancer.

OBJECTIVE:

To evaluate the growth fraction in papillomas and laryngeal squamous cell carcinomas with three degrees of differentiation through apoptosis and the expression of nucleolus organizer regions.

METHODS:

Retrospective study from which paraffin material was submitted to microtomy and hematoxylin-eosin and silver staining. Stained slides were used to quantify the apoptotic index and the number of nucleolus organizer regions by morphometry.

RESULTS:

Apoptosis was significantly more frequent in well differentiated carcinomas and in papillomas, and a higher growth fraction of expressed nucleolus organizer regions and cells that expressed a greater than average number of nucleolus organizer regions were more frequently noted in undifferentiated carcinomas.

CONCLUSIONS:

Thus, it was possible to verify that a high apoptotic index was associated with a lower chance of tumor differentiation in carcinomas, while a greater number of total nucleolus organizer regions, cells expressing nucleolus organizer regions above average and a higher growth fraction were associated with greater likelihood of abnormal cell proliferation and increased tumor differentiation.

Apoptosis; Larynx; Squamous cell carcinoma; Nucleolus organizer region; Papilloma

Groups Compared	p
SCC I × SCC II (0.10; 0.09)	0.0127^a
SCC I × SCC III (0.10; 0.08)	0.0001^a
SCC II × SCC III (0.09; 0.08)	<0.0001^a
Papilloma × SCC I (0.09; 0.10)	0.1504^a
Papilloma × SCC II (0.09; 0.09)	0.8783^a
Papilloma × SCC III (0.09; 0.08)	0.0008^a

Groups Compared	p
SCC I × SCC II (2.29; 2.76)	<0.0001^a
SCC I × SCC III (2.29; 2.99)	<0.0001^a
SCC II × SCC III (2.76; 2.99)	<0.0001^a
Papilloma × SCC I (2.53; 2.29)	0.035^a
Papilloma × SCC II (2.53; 2.76)	0.0051^a
Papilloma × SCC III (2.53; 2.99)	<0.0001^a

Groups Compared	p
SCC I × SCC II (1.87; 2.15)	<0.0001^a
SCC I × SCC III (1.87; 2.35)	<0.0001^a
SCC II × SCC III (2.15; 2.35)	<0.0001^a
Papilloma × SCC I (1.98; 1.87)	0.2021^a
Papilloma × SCC II (1.98; 2.15)	0.0053^a
Papilloma × SCC III (1.98; 2.35)	<0.0001

Groups compared	p
SCC I × SCC II (13.58; 22.50)	<0.0001^a
SCC I × SCC III (13.58; 28.21)	<0.0001^a
SCC II × SCC III (22.50; 28.21)	<0.0001^a
Papilloma × SCC I (18.70; 13.58)	0.0021^a
Papilloma × SCC II (18.70; 22.50)	0.1595^a
Papilloma × SCC III (18.70; 28.21)	<0.0001^a

Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Sede da Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico Facial, Av. Indianópolia, 1287, 04063-002 São Paulo/SP Brasil, Tel.: (0xx11) 5053-7500, Fax: (0xx11) 5053-7512 - São Paulo - SP - Brazil
E-mail: revista@aborlccf.org.br

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[1] * Corresponding author. E-mail: christianavargas@yahoo.com.br (C.V. Ribeiro).