Histometric analysis of alveolar bone regeneration with expanded polytetrafluoroethylene (e-PTFE) and latex membranes

Molina, Gustavo Otoboni; Oliveira, Marcelo Tomás de; Buss, Leonardo; Peruchi, João Davi Faraco; Pereira, Jefferson Ricardo; Ghizoni, Janaina Salomon

Abstract

AIM: To investigate the amount of connective tissue migrated into the extraction socket using EPTFE and latex membranes. METHODS: Seventeen rats were selected and randomly divided into 3 groups: e-PTFE membrane (n = 6), Latex membrane (n = 6) and Control (no membrane, n=5). After extraction of the maxillary right incisor, the animals of the test groups were subjected to alveolar guided bone regeneration (GBR) surgery and received an expanded polytetrafluoroethylene (e-PTFE) and a latex membrane, respectively. Thirty days after surgery, the animals were killed and histometric analysis was done to evaluate the migration of connective tissue. Data were analyzed statistically by one-way ANOVA and multiple-comparison Tukey's test at 5% significance level. RESULTS: There was statistically significant difference between groups e-PTFE and Latex (p=0.001), and between groups e-PTFE and Control (p=0.012), but no significant difference was found between groups Latex and Control (p=0.416). CONCLUSIONS: The e-PTFE membrane showed better results and appeared more adequate for GBR therapy, forming a barrier to prevent the migration of connective tissue into the extraction socket. The latex membrane, on the other hand, did not show benefits over the control group.

bone regeneration; membranes; oral surgery; tooth extraction

¹
Diès F, Etienne D, Abboud NB, Ouhayoun JP. Bone regeneration in extraction sites after immediate placement of an e-PTFE membrane with or without a biomaterial. A report of 12 consecutive cases. Clin Oral Impl Res. 1996; 7: 277-85.
²
Montanari M, Callea M, Yavuz I, Maglione M. A new biological approach to guided bone and tissue regeneration. BMJ Case Rep. 2013; Apr 9, 2013. doi:pii: bcr2012008240.
³
Shue L, Yufeng Z, Mony U. Biomaterials for periodontal regeneration: a review of ceramics and polymers. Biomatter. 2012; 2: 271-7.
⁴
Horowitz R, Holtzclaw D, Rosen PS. A review on alveolar ridge preservation following tooth extraction. J Evid Based Dent Pract. 2012; 12(3 Suppl): 149-60
⁵
Al Salamah L, Babay N, Anil S, Al Rasheed A, Bukhary M. Guided bone regeneration using resorbable and non-resorbable membranes: a histological study in dogs. Odontostomatol Trop. 2012; 35: 43-50.
⁶
Matsumoto G, Hoshino J, Kinoshita Y, Sugita Y, Kubo K, Maeda H, et al. Evaluation of guided bone regeneration with poly(lactic acid-co-glycolic acid-co-å-caprolactone) porous membrane in lateral bone defects of the canine mandible. Int J Oral Maxillofac Implants. 2012; 27: 587-94.
⁷
Scheyer ET, Schupbach P, McGuire MK. A histologic and clinical evaluation of ridge preservation following grafting with demineralized bonematrix, cancellous bone chips, and resorbable extracellular matrix membrane. Int J Periodontics Restorative Dent. 2012; 32: 543-52.
⁸
Macedo NL, de Macedo LG, Matuda Fde S, Ouchi SM, Monteiro AS, Carvalho YR. Guided bone regeneration with subperiosteal implants of PTFE and hydroxyapatite physical barriers in rats. Braz Dent J. 2003; 14: 199-24.
⁹
Lekovic V, Kenney EB, Weinlaender M, Han T, Klokkevold P, Nedic M, et al. A bone regenerative approach to alveolar ridge maintenance following tooth extraction. Report of 10 cases. J Periodontol. 1997;68: 563-70.
¹⁰
Melcher AH. Repair of wounds in the periodontium of the rat. Influence of periodontal ligament on osteogenesis. Arch Oral Biol. 1970; 15: 1183-204.
¹¹
Dahlin C, Linde A, Gottlow J, Nyman S. Healing of bone defects by guided tissue regeneration. Plast Reconstructive Surg. 1988; 81: 672-6.
¹²
Walters SP, Greenwell H, Hill M, Drisko C, Pickman K, Scheetz JP. Comparison of porous and non-porous teflon membranes plus a xenograft in the treatment of vertical osseous defects: A Clinical Reentry Study. J Periodontol. 2003; 74: 1161-8.
¹³
Murray C, Holden D, Roachlau W. Experimental and clinical study of new growth of bone in a cavity. Am J Surg. 1957; 95: 385-7.
¹⁴
Phillips RW, Jendresen MD, Klooster J, McNeil C, Preston JD, Schallhorn RG. Reports of the committee on scientific investigation of the American Academy of Restorative Dentistry. J Prosthetic Dent. 1990; 64: 74-110.
¹⁵
Bartee KB. Extraction Site Reconstruction For Alveolar Ridge Preservation. Part 2: Membrane-Assisted Surgical Technique. J Oral Implantol. 2001; 27: 194-7.
¹⁶
Frade MAC, Cursi, IB, Andrade FF, Netto, JC, Barbetta FMB, Foss NT. Management of diabetic skin wounds with a natural latex biomembrane. Med Cutan Iber Lat Am. 2004; 32: 157-62.
¹⁷
Mrue, F. Tissue neoformation induced by natural latex biomembrane with polylysine. Application in esophageal and abdominal wall neoformation. Experimental study in dogs [thesis]. Ribeirao Preto: University of Sao Paulo; 2000.
¹⁸
Sipos PM, Loos BG, Abbas F, Timmerman MF, van der Velden U. The combined use of enamel matrix proteins and tetracycline-coated expanded polytetrafluoroethylene barrier membrane in treatment of intra-osseous defects. J Clin Periodontol. 2004; 32: 765-72.
¹⁹
Polimeni G, Koo K-T, Qahash M, Xiropaidis AV, Albandar JM, Wikesjo¨ UME. Prognostic factors for alveolar regeneration: effect of tissue occlusion on alveolar bone regeneration with guided tissue regeneration. J Clin Periodontol. 2004; 31: 730-5.
²⁰
Hämmerle CHF, Jung RE. Bone augmentation by means of barriers membranes. Periodontol 2000. 2003; 33: 36-53.
²¹
Ereno C, Guimarães SA, Pasetto S, Herculano RD, Silva CP, Graeff CF, et al. Latex use as an occlusive membrane for guided bone regeneration. J Biomed Mater Res A. 2010; 95: 932-9.
²²
Zarkesh N, Nowzari H, Morrison JL, Slots J. Tetracycline-coated polytetrafluorethylene barrier membranes in treatment of intraosseous periodontal lesions. J Periodontol. 1999; 70: 1008-16.
²³
Schenk RK, Buser D, Hardwick WR, Dahlin C. Healing pattern of bone regeneration in membrane-protected defects: a histologic study in the canine mandible. Int J Oral Maxillofac Implants. 1994; 9: 13-29.

Publication Dates

Publication in this collection
28 Oct 2013
Date of issue
Sept 2013

History

Received
20 May 2013
Accepted
16 Aug 2013

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] ¹
Diès F, Etienne D, Abboud NB, Ouhayoun JP. Bone regeneration in extraction sites after immediate placement of an e-PTFE membrane with or without a biomaterial. A report of 12 consecutive cases. Clin Oral Impl Res. 1996; 7: 277-85.

[2] ²
Montanari M, Callea M, Yavuz I, Maglione M. A new biological approach to guided bone and tissue regeneration. BMJ Case Rep. 2013; Apr 9, 2013. doi:pii: bcr2012008240.

[3] ³
Shue L, Yufeng Z, Mony U. Biomaterials for periodontal regeneration: a review of ceramics and polymers. Biomatter. 2012; 2: 271-7.

[4] ⁴
Horowitz R, Holtzclaw D, Rosen PS. A review on alveolar ridge preservation following tooth extraction. J Evid Based Dent Pract. 2012; 12(3 Suppl): 149-60

[5] ⁵
Al Salamah L, Babay N, Anil S, Al Rasheed A, Bukhary M. Guided bone regeneration using resorbable and non-resorbable membranes: a histological study in dogs. Odontostomatol Trop. 2012; 35: 43-50.

[6] ⁶
Matsumoto G, Hoshino J, Kinoshita Y, Sugita Y, Kubo K, Maeda H, et al. Evaluation of guided bone regeneration with poly(lactic acid-co-glycolic acid-co-å-caprolactone) porous membrane in lateral bone defects of the canine mandible. Int J Oral Maxillofac Implants. 2012; 27: 587-94.

[7] ⁷
Scheyer ET, Schupbach P, McGuire MK. A histologic and clinical evaluation of ridge preservation following grafting with demineralized bonematrix, cancellous bone chips, and resorbable extracellular matrix membrane. Int J Periodontics Restorative Dent. 2012; 32: 543-52.

[8] ⁸
Macedo NL, de Macedo LG, Matuda Fde S, Ouchi SM, Monteiro AS, Carvalho YR. Guided bone regeneration with subperiosteal implants of PTFE and hydroxyapatite physical barriers in rats. Braz Dent J. 2003; 14: 199-24.

[9] ⁹
Lekovic V, Kenney EB, Weinlaender M, Han T, Klokkevold P, Nedic M, et al. A bone regenerative approach to alveolar ridge maintenance following tooth extraction. Report of 10 cases. J Periodontol. 1997;68: 563-70.

[10] ¹⁰
Melcher AH. Repair of wounds in the periodontium of the rat. Influence of periodontal ligament on osteogenesis. Arch Oral Biol. 1970; 15: 1183-204.

[11] ¹¹
Dahlin C, Linde A, Gottlow J, Nyman S. Healing of bone defects by guided tissue regeneration. Plast Reconstructive Surg. 1988; 81: 672-6.

[12] ¹²
Walters SP, Greenwell H, Hill M, Drisko C, Pickman K, Scheetz JP. Comparison of porous and non-porous teflon membranes plus a xenograft in the treatment of vertical osseous defects: A Clinical Reentry Study. J Periodontol. 2003; 74: 1161-8.

[13] ¹³
Murray C, Holden D, Roachlau W. Experimental and clinical study of new growth of bone in a cavity. Am J Surg. 1957; 95: 385-7.

[14] ¹⁴
Phillips RW, Jendresen MD, Klooster J, McNeil C, Preston JD, Schallhorn RG. Reports of the committee on scientific investigation of the American Academy of Restorative Dentistry. J Prosthetic Dent. 1990; 64: 74-110.

[15] ¹⁵
Bartee KB. Extraction Site Reconstruction For Alveolar Ridge Preservation. Part 2: Membrane-Assisted Surgical Technique. J Oral Implantol. 2001; 27: 194-7.

[16] ¹⁶
Frade MAC, Cursi, IB, Andrade FF, Netto, JC, Barbetta FMB, Foss NT. Management of diabetic skin wounds with a natural latex biomembrane. Med Cutan Iber Lat Am. 2004; 32: 157-62.

[17] ¹⁷
Mrue, F. Tissue neoformation induced by natural latex biomembrane with polylysine. Application in esophageal and abdominal wall neoformation. Experimental study in dogs [thesis]. Ribeirao Preto: University of Sao Paulo; 2000.

[18] ¹⁸
Sipos PM, Loos BG, Abbas F, Timmerman MF, van der Velden U. The combined use of enamel matrix proteins and tetracycline-coated expanded polytetrafluoroethylene barrier membrane in treatment of intra-osseous defects. J Clin Periodontol. 2004; 32: 765-72.

[19] ¹⁹
Polimeni G, Koo K-T, Qahash M, Xiropaidis AV, Albandar JM, Wikesjo¨ UME. Prognostic factors for alveolar regeneration: effect of tissue occlusion on alveolar bone regeneration with guided tissue regeneration. J Clin Periodontol. 2004; 31: 730-5.

[20] ²⁰
Hämmerle CHF, Jung RE. Bone augmentation by means of barriers membranes. Periodontol 2000. 2003; 33: 36-53.

[21] ²¹
Ereno C, Guimarães SA, Pasetto S, Herculano RD, Silva CP, Graeff CF, et al. Latex use as an occlusive membrane for guided bone regeneration. J Biomed Mater Res A. 2010; 95: 932-9.

[22] ²²
Zarkesh N, Nowzari H, Morrison JL, Slots J. Tetracycline-coated polytetrafluorethylene barrier membranes in treatment of intraosseous periodontal lesions. J Periodontol. 1999; 70: 1008-16.

[23] ²³
Schenk RK, Buser D, Hardwick WR, Dahlin C. Healing pattern of bone regeneration in membrane-protected defects: a histologic study in the canine mandible. Int J Oral Maxillofac Implants. 1994; 9: 13-29.

Brasil

Brasil

Histometric analysis of alveolar bone regeneration with expanded polytetrafluoroethylene (e-PTFE) and latex membranes

Abstract

Publication Dates

History