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Preservation of phenolic compounds on dried leaf infusion of Bauhinia forficata Link

Abstract

Bauhinia forficata Link aqueous extract is usually recommended as a phytomedicine to reduce blood glucose levels and its biological activity has been linked to the presence of phenolic compounds from B. forficata preparations. Several drying processes are used in the production of dry herbal extracts, which may influence the chemical composition and efficacy of final herbal medicines. Due to significant chemical changes, defining appropriate drying processes is essential for phytopharmaceutical drug development. In view of this, we analyzed dried B. forficata leaf infusion (BFLI) extracts by HPLC-UV-MSn, followed by molecular networking analysis to evaluate the chemical profiles from dried extracts yielded by freeze-and spray-drying processes. The main metabolites detected included 11 ferulic/isoferulic acid derivatives and 13 glycosylated flavonoids. The qualitative chemical profiles were alike for both drying processes, whereas the relative abundance of some flavonoids was higher using spray-drying. Taken together, our results showed that freeze-and spray-drying preserved the phenolic profile of BFLI and suggested that spray-drying may be the most suitable to obtain its dried products. Along with studying the chemical profiles of dried herbal extracts, evaluating the influence of drying processes on the quality and chemical profiles of final products is pivotal and may benefit future research.

Keywords:
Fabaceae; Drying process; HPLC-UV-MSn; Flavonoids; Ferulic acid derivatives.

INTRODUCTION

Bauhinia forficata Link (Fabaceae) is largely used in folk medicine as a diuretic, tonic, to reduce glycosuria, and its aqueous extract is usually recommended as a phytomedicine to reduce blood glucose levels of diabetic patients (Russo et al., 1990Russo EM, Reichelt AA, De-Sá JR, Furlanetto RP, Moisés RC, Kasamatsu TS, et al. Clinical trial of Myrcia uniflora and Bauhinia forficata leaf extracts in normal and diabetic patients. Braz J Med Biol Res. 1990;23(1):11-20.; Cechinel, 2000Cechinel-Filho V. Chemical composition and biological potential of plants from the genus Bauhinia. Phytother Res. 2000;23(10):1347-1354.; Tonelli et al., 2022Tonelli CA, Oliveira SQ, Vieira AAS, Biavatti MW, Ritter C, Reginatto FH, et al. Clinical efficacy of capsules containing standardized extract of Bauhinia forficata Link (pata-de-vaca) as adjuvant treatment in type 2 diabetes patients: A randomized, double blind clinical trial. J Ethnopharmacol. 2022;282:114616.). The hypoglycemic activity of B. forficata extracts has been related to polyphenols and flavonoids such as kaempferitrin, kaempferol, and quercetin, which are described as major compounds in B. forficata preparations (Pinheiro et al., 2006Pinheiro TS, Johansson LA, Pizzolatti MG, Biavatti MW. Comparative assessment of kaempferitrin from medicinal extracts of Bauhinia forficata link. J Pharm Biomed Anal. 2006;41:431-436.; Menezes et al., 2007Menezes FS, Minto ABM, Ruela HS, Kuster RM, Sheridan H, Frankish N. Hypoglycemic activity of two Brazilian Bauhinia species: Bauhinia forficata L. and Bauhinia monandra Kurz. Braz J Pharm. 2007;17(1):8-13.; Ferreres et al., 2012Ferreres F, Gil-Izquierdo A, Vinholes J, Silva ST, Valentão P, Andrade PB et al. Bauhinia forficata Link authenticity using flavonoids profile: relation with their biological properties. Food Chem. 2012;134(2):894-904.; Salgueiro et al. 2013Salgueiro ACF, Leal CQ, Bianchini MC, Prado IO, Mendez ASL, Puntel RL et al. The influence of Bauhinia forficata Link subsp. pruinosa tea on lipid peroxidation and non-protein SH groups in human erythrocytes exposed to high glucose concentrations. J Ethnopharmacol. 2013;148(1):81-87.).

It is well known that the increase in the number of reports of adverse reactions due to variations in the chemical composition of herbal products has attracted the attention of many regulatory agencies for the standardization of herbal formulations (Bhatta, Janezic, Ratti, 2020Bhatta S, Janezic, TS, Ratti, C. Freeze-drying of plant-based foods. Foods. 2020;9(1):87-109.; Oliveira et al., 2020Oliveira AR, Ribeiro, AEC, Oliveira, ER, Garcia MC, Soares Júnior MS, Márcio C. Structural and physicochemical properties of freeze-dried açaí pulp (Euterpe oleraceae Mart.). Food Sci Tech. 2020;40(2):282-289.). Thus, the evaluation of the chemical profile of B. forficata leaf extracts is essential to guarantee the quality from pharmaceutical preparations produced with this herbal medicine.

Drying techniques such as freeze-and spray-drying are preferably used in the production of dry herbal extracts with the goal of preserving the active compounds, as well as assure stability, safety of use, and prolong storage. In contrast, significant physical and chemical changes in the composition can occur in the drying processes that applied higher temperatures, resulting in the loss or degradation of heat-sensitive compounds. Furthermore, phenolic compounds are easily susceptible to enzymatic degradation and are highly unstable during storage and processing (Sollohub, Cal, 2010Sollohub K, Cal K. Spray drying technique: II. Current applications in pharmaceutical technology. J Pharm Sci. 2010;99(2):587-597.; Kasper, Winter, Friess, 2013Kasper JC, Winter G, Friess, W. Recent advances and further challenges in lyophilization. Eur J Pharm Biopharm. 2013;85(2):162-169.).

In the last few years, researchers have investigated the influence of drying processes on the chemical properties of B. forficata hydroalcoholic extracts (Oliveira, Bott, Souza, 2006Oliveira WP, Bott RF, Souza CRF. Manufacture of standardized dried extracts from medicinal Brazilian plants. Drying Tech. 2006;24(4):523-533.; Souza, Oliveira, 2006Souza CRF, Oliveira WP. Powder properties and system behavior during spray drying of Bauhinia forficata Link Extract. Drying Techl. 2006;24(6):735-749.; Souza et al., 2015Souza CRF, Fernandes LP, Bott RF, Oliveira WP. Influência do processo de secagem e condição de armazenamento de extratos secos de Bauhinia forficata e Passiflora alata sobre seu perfil de dissolução. Rev Bras Plant Med. 2015;17(1):67-75.). However, it is still not known whether drying processes affect the phenolic composition of B. forficata leaf aqueous extract (infusion). Therefore, the aim of this work was to evaluate the impact of freeze-and spray-drying processes on the chemical profile from B. forficata leaf infusion (BFLI) by liquid chromatography coupled to mass spectrometry (LC-MS)-based molecular networking analysis to aid the annotation of metabolites and comparison of their relative abundances after each drying process.

MATERIAL AND METHODS

Infusion preparation and drying processes

B. forficata leaves were collected in the Botanic Garden of Medicinal Plant Ordem e Progresso in Jurucê, a district of the city of Jardinópolis (São Paulo, Brazil). A voucher specimen was deposited at the Medicinal Plants Herbarium of the University of Ribeirão Preto (HPM-UNAERP, Ribeirão Preto, SP, Brazil) under number 1793. The utilization of B. forficata samples in this study was authorized by the Council for the Genetic Heritage Management (CGEN)/Ministry of Environment (Sisgen/ register no. A093CD8) through the office of the National Council for Scientific and Technological Development (CNPq). Dried leaves (2 kg) were powdered and added to boiling water (10 L) and allowed to stand in a capped glass container for 1 hour. The infusion was filtered through analytical filter papers under vacuum. The aqueous phase was divided into two portions; one portion (3.25 L) was submitted to freeze-drying (Flexi-Dry MPTM Freeze Dryer, FTS Systems, USA) to produce freeze-dried BFLI; whereas the other (1.61 L) was subjected to spray-drying (SD-Basic Laboratory Scale Spray Dryer, LabPlant, United Kingdom) to produce spray-dried BFLI. For the freeze-drying process, the aqueous phase was frozen at-40 °C and freeze dried under vacuum at 140 L.min-1 for 48 hours. The spray-drying process was carried out with an inlet temperature of 160 ± 1 °C, outlet air temperature of ± 80 °C, and pump flow rate of 8 mL.min-1. The resulting dried powders were stored at-4 oC until further analysis.

High-performance liquid chromatography (HPLC) coupled to ultraviolet diode array detection (UV-DAD) and electrospray ionization mass spectrometry (ESI-MS)

Samples were prepared at a concentration of 1 mg/ mL of each freeze-or spray-dried powder of BFLI in MeOH:H2O (1:1, v/v). The samples were vortex agitated and filtered by a 0.45 µm PTFE filter before analysis. The analyses were obtained in a high-performance liquid chromatography (HPLC) system (Shimadzu Prominence LC-20A, Shimadzu Corporation, Kyoto, Japan) equipped with diode array detector (DAD) and coupled with a mass spectrometer with electrospray ionization source and analyzer ion trap (AmaZon SL, Bruker Daltonics, Billerica, MA, USA). A C18 chromatographic column (Luna, 5 µm, 250 x 4.6 mm; Phenomenex, Macclesfield, United Kingdom) was used and a total of 20 L of each sample was injected. The mobile phase was composed of ultrapure water and MeOH (both containing 1% acetic acid) and a flow rate of 1 mL.min-1 was applied. The gradient of elution of the mobile phase and all other chromatographic parameters were the same as those described by Ferreres et al. (2012Ferreres F, Gil-Izquierdo A, Vinholes J, Silva ST, Valentão P, Andrade PB et al. Bauhinia forficata Link authenticity using flavonoids profile: relation with their biological properties. Food Chem. 2012;134(2):894-904.). UV spectral data were recorded in the range 240-400 nm. Mass spectrometry data were acquired in negative and positive ionization modes, separately, employing the following parameters: capillary voltage, 3.5 kV; end plate offset, 500 V; nebulizer, 50 psi; dry gas (N2 ) flow, 9 L·min−1; dry temperature, 300 oC; auto MS/MS acquiring data between m/z 50 and 1200, average of three spectra; enhanced resolution for scan mode and UltraScan mode for MS/ MS; spectra rate acquisition, three spectra/s; exclusion of a particular ion after three spectra and released after 30 s. The mass spectrometer was controlled by Hystar software (Bruker Daltonics Inc., Billerica, MA, USA), and chromatograms and mass spectra were visualized using Data Analysis software (Shimadzu Corporation, Kyoto, Japan).

Annotation of the chemical compounds

LC-MS data from positive and negative ionization modes were converted to .mzXML format by MSConvert software (Proteowizard Software Foundation, USA) and processed by MzMineTM (version 2.51, BMC Bioinformatics, United Kingdom). The following parameters were set for data processing: mass detection using the centroid algorithm, scan MS level 1 (noise level, 1.0E5) and scan MS level 2 (noise level, 1.0E4); Automatied Data Analysis Pipeline (ADAP) chromatogram builder (min group size in # of scans, 5; group intensity threshold, 1.0E5; min highest intensity, 1.0E6; m/z tolerance, 0.3 m/z or 200 ppm); chromatogram deconvolution using wavelets (ADAP) algorithm (S/N threshold, 10; S/N estimator, intensity window SN; min feature height, 1.0E6; coefficient/ area threshold, 5; peak duration range, 0.2 - 2.00; RT wavelet range, 0.02 - 0.20) (m/z center calculation - median; m/z range for MS2 scan pairing, 0.3; RT for MS2 scan pairing, 0.2); isotopic peak grouper (m/z tolerance, 0.3 m/z or 200 ppm; retention time tolerance in minutes, 0.2; maximum charge, 2; representative isotope, most intense); and alignment using the join aligner (m/z tolerance, 0.3 m/z or 200 ppm; weight for m/z, 50; retention time tolerance, 0.3 (abs); weight for retention time, 50). After data processing, peaks with MS2 scan were exported for GNPS (Global Natural Products Social Molecular Networking) analysis as a .csv quantification spreadsheet and as a .mgf file.

The output data from positive and negative ionization modes were uploaded to the GNPS platform (https://gnps.ucsd.edu/ProteoSAFe/static/gnps-splash. jsp) and the “advanced analysis tools for feature networking” were used to generate the molecular networking (Nothias et al., 2020Nothias LF, Petras D, Schmid R, Dührkop K, Rainer J, Sarvepalli A et al. Feature-based molecular networking in the GNPS analysis environment. Nat Methods 2020;17(9):905-908.; Wang et al., 2016Wang M, Carver JJ, Phelan VV, Sanchez LM, Garg N, Peng Y et al. Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking. Nat Biotechnol. 2016;34(8):828-837.). The following parameters were employed: precursor ion mass tolerance, 2.0 Da; fragment ion mass tolerance, 0.5 Da; minutes pairs cos, 0.6; minimum matched fragment ions, 4; maximum shift between precursors, 500 Da; network topK 10; maximum connected component size, 100; library search minutes matched peaks, 4; score threshold, 0.7; search analogues, don’t search; maximum analog search mass difference, 100 Da; top results to report per query, 1; minimum peak intensity, 0; filter precursor window, filter; filter library; filter peaks in 500 Da window, filter; normalization per file, no norm; aggregation method for peak abundances per group, sum. Finally, molecular networking was generated and analyzed with Cytoscape (version 3.7.2, Institute for Systems Biology, Seattle, WA, USA). Nodes in the molecular network were represented as pie charts, which were used to compare relative abundances of metabolites in freeze-and spray-dried samples.

Hit compounds pointed by the GNPS spectral library were compared with additional information (UV spectra and MS fragmentation patterns) and then annotated. Sugar moieties indicated by the GNPS library were characterized as hexosides, pentosides, deoxyhexosides, etc. (i.e., stereochemistry was not considered in the compound annotation). Isomers were differentiated based on retention times, since the exact positions of the sugar moieties could not be determined by the techniques implemented here (i.e., positions of the sugar moieties were not assigned for the annotated compounds).

All raw LC-MSn data and MzMine exported files were deposited and can be accessed via the MassIVE submission (MSV000089571; Creative Commons CC0 1.0 Universal license).

RESULTS AND DISCUSSION

The chemical profiles of freeze-and spray-dried BFLI were characterized mainly by the presence of phenolic compounds, including 11 hydroxyferulic/ isoferulic acids (FA) derivatives and 13 glycosylated flavonoids (Table I).

TABLE I
Annotated compounds in freeze-and spray-dried BFLI

The basic skeletons of the annotated FA derivatives and flavonoids are presented in Figure 1. All of the annotated metabolites were detected in freeze-and spray-dried BFLI. Therefore, the drying conditions used here did not significantly modify the qualitative chemical profile of dried BFLI with respect to the phenolic constituents.

FIGURE 1
Basic skeletons of hydroxyferulic/isoferulic acids (A) and flavonoids (B).

FA derivatives are hydroxycinnamic acids commonly found in plants and they exhibit potential therapeutic effects in the pharmaceutical field, and may occur free, dimerized, or conjugated with sugars (Paiva et al., 2013Paiva LB, Goldbeck R, Santos WD, Squina FM. Ferulic acid and derivatives: Molecules with potential application in the pharmaceutical field. Braz J Pharm Sci. 2013;49(3):395-411.). Regarding the LC-MS-based molecular networking analysis, FA derivatives were detected mostly in negative ionization mode. Comparing the relative abundance of the annotated metabolites in the molecular networking (Figure 2), we observed that freeze-and spray-dried BFLI exhibited alike relative abundances for the annotated FA derivatives.

FIGURE 2
Molecular networking of freeze-and spray-dried BFLI constructed by LC-MS data obtained in negative ionization mode. Each node represents a metabolite, which was detected in freeze-dried (blue) and/or spray-dried (yellow) samples. The annotated ferulic/isoferulic acid derivatives are highlighted as larger octagonal nodes.

Flavonoids are phenolic compounds chemically characterized by a 15-carbon phenyl-benzopyrone skeleton. According to the patterns of hydroxylation and glycosylation, as well as degree of unsaturation and oxidation, flavonoids are classified into several classes (e.g., flavones, flavonols, flavanones, flavanonols, isoflavonoids, flavan-3-ols, chalcones, aurones, and anthocyanins) and occur as aglycones, glycosides and/or methylated derivatives (Kumar, Pandey, 2013Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Sci World J. Volume 2013;Article ID 162750:16 pages.; Dias, Pinto, Silva, 2021Dias MC, Pinto DCGA, Silva AMS. Plant flavonoids: chemical characteristics and biological activity. Molecules. 2021;26(5377):1-16.). In this study, freeze-and spray-dried BFLI were characterized mainly by the presence of glycosylated flavonols (i.e., kaempferol and quercetin conjugated with sugar moieties). Additionally, a glycosylated flavone (luteolin conjugated with a sugar group) was also detected in both samples (Figures S1 and S2, Supplementary Material).

The molecular networking generated with LC-MS data obtained in positive ionization mode was constituted mainly by annotated flavonoids (Figure 3). The relative abundances of some flavonoids were equivalent in freeze-and spray-dried samples. However, other flavonoids, including quercetin O-hexosyl-deoxyhexosyl-hexoside (13), quercetin O-di-hexoside (14), kaempferol O-di-hexoside (17), quercetin O-deoxyhexoside (21) and kaempferol O-deoxyhexoside (24), exhibited higher relative abundances in the spray-dried sample.

FIGURE 3
Molecular networking of freeze-and spray-dried BFLI constructed by HPLC-MS data obtained in positive ionization mode. Each node represents a metabolite, which was detected in freeze-dried (blue) and/or spray-dried (yellow) samples. The annotated flavonoids are highlighted as larger octagonal nodes.

These findings are consistent with previous reports that described free and glycosylated forms of kaempferol and quercetin as the most frequent secondary metabolites isolated from B. forficata leaves (Ferreres et al., 2012Ferreres F, Gil-Izquierdo A, Vinholes J, Silva ST, Valentão P, Andrade PB et al. Bauhinia forficata Link authenticity using flavonoids profile: relation with their biological properties. Food Chem. 2012;134(2):894-904.; Farias, Mendez, 2014Farias LS, Mendez ASL. LC/ESI-MS method applied to characterization of flavonoids glycosides in B. forficata subsp. pruinosa. Quim Nova. 2014;37(3):483-486.; Miceli et al., 2016Miceli N, Buongiorno LP, Celi MG, Cacciola F, Dugo P, Donato P et al. Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract. Nat Prod Res. 2016;30(11):1229-39.). Compared with the chemical composition reported for the aqueous B. forficata extracts (Menezes et al., 2007Menezes FS, Minto ABM, Ruela HS, Kuster RM, Sheridan H, Frankish N. Hypoglycemic activity of two Brazilian Bauhinia species: Bauhinia forficata L. and Bauhinia monandra Kurz. Braz J Pharm. 2007;17(1):8-13.; Salgueiro et al., 2013Salgueiro ACF, Leal CQ, Bianchini MC, Prado IO, Mendez ASL, Puntel RL et al. The influence of Bauhinia forficata Link subsp. pruinosa tea on lipid peroxidation and non-protein SH groups in human erythrocytes exposed to high glucose concentrations. J Ethnopharmacol. 2013;148(1):81-87.), the analytical technique employed here increased the variety of detected flavonoids and allowed characterization of FA derivatives on freeze-and spray-dried BFLI. Figures S3 and S4 (Supplementary Material) show the peak area of compounds annotated from freeze-and spray-dried BFLI obtained in positive and negative ionization modes.

Although the freeze-drying process is a preferred method for drying liquid samples containing thermally sensitive compounds, such as phenolic substances (Bhatta, Janezic, Ratti, 2020Bhatta S, Janezic, TS, Ratti, C. Freeze-drying of plant-based foods. Foods. 2020;9(1):87-109.; Oliveira et al., 2020Oliveira AR, Ribeiro, AEC, Oliveira, ER, Garcia MC, Soares Júnior MS, Márcio C. Structural and physicochemical properties of freeze-dried açaí pulp (Euterpe oleraceae Mart.). Food Sci Tech. 2020;40(2):282-289.), our results showed that spray-dried infusion of B. forficata leaves exhibited higher relative abundance of some flavonoids compared to the freeze-dried infusion. Additionally, the spray-drying process was carried out within two hours, while the freeze-drying process required 48 hours for water removal. These results suggest that spray-drying may be the most suitable drying process to obtain dried aqueous extracts of B. forficata leaves, whereas, both drying processes showed similar relative abundance for the FA derivatives. Similar findings were observed in a study developed by Gomes et al. (2018Gomes WF, França FRM, Denadai M, Andrade, JKS, Oliveira SEM et. al . Effect of freeze-and spray-drying on physico-chemical characteristics, phenolic compounds, and antioxidant activity of papaya pulp. J Food Sci Tech. 2018;55(6):2095-2102.), which reported slightly high concentrations of polyphenolic compounds in spray-dried papaya product than the freeze-dried product.

Horszwald, Julien, Andlauer (2013Horszwald A, Andlauer JHW. Characterisation of Aronia powders obtained by different drying processes. Food Chem. 2013;141(3):2858-2863.), also described a higher content of total flavonoids in Aronia powders in spray-drying samples than freeze-drying. Thus, the spray-drying process proved to be very advantageous for drying Aronia commercial juice, due to its faster drying, reducing the hydrolysis process and consequently preserving the integrity of the glycosylated compounds. The application of high temperature during spray-drying processes has been related to deactivation of oxidative and hydrolytic enzymes, consequently avoiding the loss of polyphenolic components (Sukrasno et al., 2011Sukrasno S, Fidriany I, Anggadiredja K, Handayani WA, Anam K. Influence of drying method on flavonoid content of Cosmos caudatus (Kunth) leaves. Res J Med Plant. 2011;5(2):189-195.; Horszwald, Julien, Andlauer, 2013Horszwald A, Andlauer JHW. Characterisation of Aronia powders obtained by different drying processes. Food Chem. 2013;141(3):2858-2863.; Gomes et al., 2018Gomes WF, França FRM, Denadai M, Andrade, JKS, Oliveira SEM et. al . Effect of freeze-and spray-drying on physico-chemical characteristics, phenolic compounds, and antioxidant activity of papaya pulp. J Food Sci Tech. 2018;55(6):2095-2102.).

Drying conditions used in this work did not extensively modify the qualitative phenolic profile of freeze-and spray-dried BFLI. Thus, our results showed that both freeze-and spray-drying processes qualitatively preserved the phenolic compounds of BFLI and were suitable to produce dried aqueous extract of B. forficata Link. Along with the preservation of the phenolic compounds of dried herbal extracts, evaluating the influence of drying processes on the quality of the final products is pivotal and may benefit future researches.

ACKNOWLEDGEMENTS

This study was supported by The Foundation for Research Support on Herbal Medicines (FUNFITO, UNAERP, Brazil) and the São Paulo Research Foundation (FAPESP), grant #2017/17023-4.

REFERENCES

  • Bhatta S, Janezic, TS, Ratti, C. Freeze-drying of plant-based foods. Foods. 2020;9(1):87-109.
  • Cechinel-Filho V. Chemical composition and biological potential of plants from the genus Bauhinia. Phytother Res. 2000;23(10):1347-1354.
  • Dias MC, Pinto DCGA, Silva AMS. Plant flavonoids: chemical characteristics and biological activity. Molecules. 2021;26(5377):1-16.
  • Farias LS, Mendez ASL. LC/ESI-MS method applied to characterization of flavonoids glycosides in B. forficata subsp. pruinosa. Quim Nova. 2014;37(3):483-486.
  • Ferreres F, Gil-Izquierdo A, Vinholes J, Silva ST, Valentão P, Andrade PB et al. Bauhinia forficata Link authenticity using flavonoids profile: relation with their biological properties. Food Chem. 2012;134(2):894-904.
  • Gomes WF, França FRM, Denadai M, Andrade, JKS, Oliveira SEM et. al . Effect of freeze-and spray-drying on physico-chemical characteristics, phenolic compounds, and antioxidant activity of papaya pulp. J Food Sci Tech. 2018;55(6):2095-2102.
  • Horszwald A, Andlauer JHW. Characterisation of Aronia powders obtained by different drying processes. Food Chem. 2013;141(3):2858-2863.
  • Kasper JC, Winter G, Friess, W. Recent advances and further challenges in lyophilization. Eur J Pharm Biopharm. 2013;85(2):162-169.
  • Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Sci World J. Volume 2013;Article ID 162750:16 pages.
  • Menezes FS, Minto ABM, Ruela HS, Kuster RM, Sheridan H, Frankish N. Hypoglycemic activity of two Brazilian Bauhinia species: Bauhinia forficata L. and Bauhinia monandra Kurz. Braz J Pharm. 2007;17(1):8-13.
  • Miceli N, Buongiorno LP, Celi MG, Cacciola F, Dugo P, Donato P et al. Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract. Nat Prod Res. 2016;30(11):1229-39.
  • Nothias LF, Petras D, Schmid R, Dührkop K, Rainer J, Sarvepalli A et al. Feature-based molecular networking in the GNPS analysis environment. Nat Methods 2020;17(9):905-908.
  • Oliveira WP, Bott RF, Souza CRF. Manufacture of standardized dried extracts from medicinal Brazilian plants. Drying Tech. 2006;24(4):523-533.
  • Oliveira AR, Ribeiro, AEC, Oliveira, ER, Garcia MC, Soares Júnior MS, Márcio C. Structural and physicochemical properties of freeze-dried açaí pulp (Euterpe oleraceae Mart.). Food Sci Tech. 2020;40(2):282-289.
  • Paiva LB, Goldbeck R, Santos WD, Squina FM. Ferulic acid and derivatives: Molecules with potential application in the pharmaceutical field. Braz J Pharm Sci. 2013;49(3):395-411.
  • Pinheiro TS, Johansson LA, Pizzolatti MG, Biavatti MW. Comparative assessment of kaempferitrin from medicinal extracts of Bauhinia forficata link. J Pharm Biomed Anal. 2006;41:431-436.
  • Russo EM, Reichelt AA, De-Sá JR, Furlanetto RP, Moisés RC, Kasamatsu TS, et al. Clinical trial of Myrcia uniflora and Bauhinia forficata leaf extracts in normal and diabetic patients. Braz J Med Biol Res. 1990;23(1):11-20.
  • Salgueiro ACF, Leal CQ, Bianchini MC, Prado IO, Mendez ASL, Puntel RL et al. The influence of Bauhinia forficata Link subsp. pruinosa tea on lipid peroxidation and non-protein SH groups in human erythrocytes exposed to high glucose concentrations. J Ethnopharmacol. 2013;148(1):81-87.
  • Sollohub K, Cal K. Spray drying technique: II. Current applications in pharmaceutical technology. J Pharm Sci. 2010;99(2):587-597.
  • Souza CRF, Oliveira WP. Powder properties and system behavior during spray drying of Bauhinia forficata Link Extract. Drying Techl. 2006;24(6):735-749.
  • Souza CRF, Fernandes LP, Bott RF, Oliveira WP. Influência do processo de secagem e condição de armazenamento de extratos secos de Bauhinia forficata e Passiflora alata sobre seu perfil de dissolução. Rev Bras Plant Med. 2015;17(1):67-75.
  • Sukrasno S, Fidriany I, Anggadiredja K, Handayani WA, Anam K. Influence of drying method on flavonoid content of Cosmos caudatus (Kunth) leaves. Res J Med Plant. 2011;5(2):189-195.
  • Tonelli CA, Oliveira SQ, Vieira AAS, Biavatti MW, Ritter C, Reginatto FH, et al. Clinical efficacy of capsules containing standardized extract of Bauhinia forficata Link (pata-de-vaca) as adjuvant treatment in type 2 diabetes patients: A randomized, double blind clinical trial. J Ethnopharmacol. 2022;282:114616.
  • Wang M, Carver JJ, Phelan VV, Sanchez LM, Garg N, Peng Y et al. Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking. Nat Biotechnol. 2016;34(8):828-837.

Publication Dates

  • Publication in this collection
    15 May 2023
  • Date of issue
    2023

History

  • Received
    26 Jan 2022
  • Accepted
    10 Nov 2022
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Av. Prof. Lineu Prestes, n. 580, 05508-000 S. Paulo/SP Brasil, Tel.: (55 11) 3091-3824 - São Paulo - SP - Brazil
E-mail: bjps@usp.br