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Soybean and bean germoplasm characterization by electrophoresis of seed isoenzymes

Seed isoenzymes of two soybean cultivars, IAC 6 and IAC 9, and two lines of the bean cultivar Goiano Precoce, one with smooth leaves and the other with wrinkled leaves, were studied through electrophoresis. The plants of soybean cultivars differed for the isoenzymes urease, acid phosphatase, malic dehydrogenase and leucine aminopeptidase. The results indicate that the two cultivars of soybean are very different genetically, despite their origin from a single, older variety. The eletrophoretic characters which differed between the two soybean cultivars may have potentiality for discriminating them among other cultivars. On the other hand, the lines of these bean cultivars did not differ in any of the analysed characters that would indicate a very close relationship between them. These results are consistent with the hypothesis, from morphological data, that wrinkled leaves Goiano Precoce could be originated from the other line with smooth leaves through a point mutation.

soybean; bean; electrophoresis; isoenzymes; cultivars; characterization


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