<i>Rpp</i> genes conferring resistance to Asian soybean rust in F<sub>2</sub> population in the field conditions

Meira, Daniela; Batti, Vinícius de Bitencourt Bez; Woyann, Leomar Guilherme; Milioli, Anderson Simionato; Bozi, Antonio Henrique; Beche, Eduardo; Panho, Maiara Cecilia; Barrinouevo, Fabiana; Madella, Laura Alexandra; Malone, Gaspar; Brito Júnior, Salvador Lima; Finatto, Taciane; Benin, Giovani

doi:10.1590/1678-4499.20230099

Daniela Meira

Conceptualization

Methodology

Investigation

Writing – original draft

Writing – review & editing

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0001-7843-4472

Vinícius de Bitencourt Bez Batti

Conceptualization

Methodology

Investigation

Writing – original draft

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0002-6210-2518

Leomar Guilherme Woyann

Writing – original draft

Writing – review & editing

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0002-7707-7984

Anderson Simionato Milioli

Investigation

Writing – original draft

Writing – review & editing

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0002-7000-0140

Antonio Henrique Bozi

Investigation

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0001-9663-593X

Eduardo Beche

Writing – review & editing

University of Missouri – Division of Plant Science, Columbia, Missouri, MO 65211, USA.

http://orcid.org/0000-0003-2318-3615

Maiara Cecilia Panho

Methodology

Writing – original draft

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0002-4747-4559

Fabiana Barrinouevo

Investigation

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0002-3081-7543

Laura Alexandra Madella

Investigation

Writing – original draft

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0001-9440-8266

Gaspar Malone

Supervision

GDM Seeds – Londrina (PR), Brazil.

http://orcid.org/0000-0003-2812-063X

Salvador Lima Brito Júnior

Supervision

GDM Seeds – Londrina (PR), Brazil.

http://orcid.org/0000-0002-0737-9186

Taciane Finatto

Writing – review & editing

Supervision

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0002-6873-3783

Giovani Benin ^**Corresponding author: benin@utfpr.edu.br

Supervision

Universidade Federal do Paraná – Setor de Ciências Agrárias – Departamento de Fitotecnia e Fitossanidade – Curitiba (PR), Brazil.

http://orcid.org/0000-0002-7354-5568

*Corresponding author: benin@utfpr.edu.br

Section Editor: Carlos Alberto Scapim https://orcid.org/0000-0002-7047-9606

Population	Crop Season	Susceptible parental	Resistance source (PI number) – Rpp	Chr	Origin⁹ 9 GRIN - Germplasm Resources Information Network. Natl. Germplasm Resource. https://www.ars-grin.gov/
1 – Rpp1	2017/18	55I57RSF IPRO	Komata (PI 200492) – Rpp1¹ 1 PI 200492 – Hyten et al. (2007).	18	Japan
2 – Rpp1-b	2017/18	55I57RSF IPRO	(PI 594538A) – Rpp1-b² 2 PI 594538A - Chakraborty et al. (2009).	18	China
3 – Rpp1-b	2017/18	55I57RSF IPRO	Huang Dou (PI 587880A) – Rpp1-b³ 3 PI 587880A- Ray et al. (2009).	18	China
4 – Rpp1¹ * PI 594723 carrying an Rpp1 allele not mapped until this moment.	2017/18	55I57RSF IPRO	He xian hei dou (PI 594723) - Rpp1¹ * PI 594723 carrying an Rpp1 allele not mapped until this moment. ⁴ 4 PI 594723 – Miles et al. (2006);	18	China
5 – Rpp1¹ * PI 594723 carrying an Rpp1 allele not mapped until this moment.	2018/19	63I64RSF IPRO	He xian hei dou (PI 594723) - Rpp1¹ * PI 594723 carrying an Rpp1 allele not mapped until this moment. ⁴ 4 PI 594723 – Miles et al. (2006);	18	China
6 – Rpp2	2018/19	55I57RSF IPRO	PI 230970 - Rpp2⁵ 5 PI 230970 – Hartwig and Bromfield (1983);	16	Japan
7 – Rpp3, 5	2018/19	55I57RSF IPRO	Hyuuga (PI 506764) - Rpp3, Rpp5⁶ 6 PI 506764 – Kendrick et al. (2011);	6, 3	Japan
8 – Rpp4	2018/19	55I57RSF IPRO	Bing nan (PI 459025A) - Rpp4⁷ 7 PI 459025A – Hartwig (1986);	18	China
9 – Rpp5	2018/19	55I57RSF IPRO	Kinoshita (PI 200487) - Rpp5⁸ 8 PI 200487 – Garcia et al. (2008);	3	Japan
10 – Rpp5	2018/19	63I64RSF IPRO	Kinoshita (PI 200487) - Rpp5⁸ 8 PI 200487 – Garcia et al. (2008);	3	Japan

¹
PI 200492 – Hyten et al. (2007)Hyten, D.L., Hartman, G.L., Nelson, R.L., Frederick, R.D., Concibido, V.C., Narvel, J.M. and Cregan, P.B. (2007). Map Location of the Rpp1 Locus That Confers Resistance to Soybean Rust in Soybean. Crop Science, 47, 837–838. https://doi.org/10.2135/cropsci2006.07.0484
https://doi.org/10.2135/cropsci2006.07.0... .
²
PI 594538A - Chakraborty et al. (2009)Chakraborty, N., Curley, J., Frederick, R.D., Hyten, D.L., Nelson, R.L., Hartman, G.L. and Diers, B.W. (2009). Mapping and Confirmation of a New Allele at Rpp1 from Soybean PI 594538A Conferring RB Lesion–Type Resistance to Soybean Rust. Crop Science, 49, 783–790. https://doi.org/10.2135/cropsci2008.06.0335
https://doi.org/10.2135/cropsci2008.06.0... .
³
PI 587880A- Ray et al. (2009)Ray, J.D., Morel, W., Smith, J.R., Frederick, R.D. and Miles, M.R. (2009). Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A. Theoretical Applied Genetic, 119, 271–280. https://doi.org/10.1007/s00122-009-1036-z
https://doi.org/10.1007/s00122-009-1036-... .
⁴
PI 594723 – Miles et al. (2006);
⁵
PI 230970 – Hartwig and Bromfield (1983)Hartwig, E.E. and Bromfield, K.R. (1983). Relationships Among Three Genes Conferring Specific Resistance to Rust in Soybeans. Crop Science, 23. https://doi.org/10.2135/cropsci1983.0011183X002300020012x
https://doi.org/10.2135/cropsci1983.0011... ;
⁶
PI 506764 – Kendrick et al. (2011)Kendrick, M.D., Harris, D.K., Ha, B.K., Hyten, D.L., Cregan, P.B., Frederick, R.D., Boerma, H.R. and Pedley, K.F. (2011). Identification of a Second Asian Soybean Rust Resistance Gene in Hyuuga Soybean. Phytopathology, 101, 535–543. https://doi.org/10.1094/PHYTO-09-10-0257
https://doi.org/10.1094/PHYTO-09-10-0257... ;
⁷
PI 459025A – Hartwig (1986)Hartwig, E.E. (1986). Identification of a Fourth Major Gene Conferring Resistance to Soybean Rust. Crop Science, 26. https://doi.org/10.2135/cropsci1986.0011183X002600060010x
https://doi.org/10.2135/cropsci1986.0011... ;
⁸
PI 200487 – Garcia et al. (2008)Garcia, A., Calvo, É.S., Souza K.R.A., Harada, A., Hiromoto, D.M. and Vieira, L.G.E. (2008). Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles. Theoretical Applied Genetic, 117, 545–553. https://doi.org/10.1007/s00122-008-0798-z
https://doi.org/10.1007/s00122-008-0798-... ;
⁹
GRIN - Germplasm Resources Information Network. Natl. Germplasm Resource. https://www.ars-grin.gov/
^*
PI 594723 carrying an Rpp1 allele not mapped until this moment.
Source: Elaborated by the authors using data from Hyten et al. (2007)Hyten, D.L., Hartman, G.L., Nelson, R.L., Frederick, R.D., Concibido, V.C., Narvel, J.M. and Cregan, P.B. (2007). Map Location of the Rpp1 Locus That Confers Resistance to Soybean Rust in Soybean. Crop Science, 47, 837–838. https://doi.org/10.2135/cropsci2006.07.0484
https://doi.org/10.2135/cropsci2006.07.0... , Chakraborty et al. (2009)Chakraborty, N., Curley, J., Frederick, R.D., Hyten, D.L., Nelson, R.L., Hartman, G.L. and Diers, B.W. (2009). Mapping and Confirmation of a New Allele at Rpp1 from Soybean PI 594538A Conferring RB Lesion–Type Resistance to Soybean Rust. Crop Science, 49, 783–790. https://doi.org/10.2135/cropsci2008.06.0335
https://doi.org/10.2135/cropsci2008.06.0... , Ray et al. (2009)Ray, J.D., Morel, W., Smith, J.R., Frederick, R.D. and Miles, M.R. (2009). Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A. Theoretical Applied Genetic, 119, 271–280. https://doi.org/10.1007/s00122-009-1036-z
https://doi.org/10.1007/s00122-009-1036-... , Miles et al. (2006), Hartwig and Bromfield (1983)Hartwig, E.E. and Bromfield, K.R. (1983). Relationships Among Three Genes Conferring Specific Resistance to Rust in Soybeans. Crop Science, 23. https://doi.org/10.2135/cropsci1983.0011183X002300020012x
https://doi.org/10.2135/cropsci1983.0011... , Kendrick et al. (2011)Kendrick, M.D., Harris, D.K., Ha, B.K., Hyten, D.L., Cregan, P.B., Frederick, R.D., Boerma, H.R. and Pedley, K.F. (2011). Identification of a Second Asian Soybean Rust Resistance Gene in Hyuuga Soybean. Phytopathology, 101, 535–543. https://doi.org/10.1094/PHYTO-09-10-0257
https://doi.org/10.1094/PHYTO-09-10-0257... , Hartwig (1986)Hartwig, E.E. (1986). Identification of a Fourth Major Gene Conferring Resistance to Soybean Rust. Crop Science, 26. https://doi.org/10.2135/cropsci1986.0011183X002600060010x
https://doi.org/10.2135/cropsci1986.0011... , Garcia et al. (2008)Garcia, A., Calvo, É.S., Souza K.R.A., Harada, A., Hiromoto, D.M. and Vieira, L.G.E. (2008). Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles. Theoretical Applied Genetic, 117, 545–553. https://doi.org/10.1007/s00122-008-0798-z
https://doi.org/10.1007/s00122-008-0798-... , 9GRIN - Germplasm Resources Information Network. Natl. Germplasm Resource. https://www.ars-grin.gov/.

Pop - Rpp gene	Crossing	Number of plants			χ² R:S (3:1)	χ² p(<0.05)
Pop - Rpp gene	Crossing	R	S	Total	χ² R:S (3:1)	χ² p(<0.05)
P1 - Rpp1	55I57RSF IPRO x PI 200492	27	27	54	6.75	0.00
P2 - Rpp1-b	55I57RSF IPRO x PI 594538A	59	16	75	0.20	0.46
P3 - Rpp1-b	55I57RSF IPRO x PI 587880A	55	15	70	0.18	0.49
P4 - Rpp1*	55I57RSF IPRO x PI 594723	54	15	69	0.15	0.53
P5 - Rpp1*	63I64RSF IPRO x PI 594723	210	88	298	3.26	0.07
P6 - Rpp2	55I57RSF IPRO x PI 230970	172	105	277	24.61	0.00
P7 - Rpp3,5	55I57RSF IPRO x PI 506764	213	75	288	10.05¹ 1 Segregation expected 13:3. Source: Elaborated by the authors.	0.00
P8 - Rpp4	55I57RSF IPRO x PI 459025A	261	30	291	69.42	0.00
P9 - Rpp5	55I57RSF IPRO x PI 200487	226	58	284	3.17	0.07
P10 - Rpp5	63I64RSF IPRO x PI 200487	230	54	284	5.43	0.02

PI	Rpp	Chr	Marker start – end (physical position)		Distance (kb)	Reference
PI 200492	Rpp1	18	Sat_187 (56181759)	Sat_064 (56333703)	152.1	Hyten et al. 2007Hyten, D.L., Hartman, G.L., Nelson, R.L., Frederick, R.D., Concibido, V.C., Narvel, J.M. and Cregan, P.B. (2007). Map Location of the Rpp1 Locus That Confers Resistance to Soybean Rust in Soybean. Crop Science, 47, 837–838. https://doi.org/10.2135/cropsci2006.07.0484 https://doi.org/10.2135/cropsci2006.07.0...
PI 594538A	Rpp1-b	18	Sat_064 (56333703)	Sat_372 (56333845)	463.5	Chakraborty et al. 2009Chakraborty, N., Curley, J., Frederick, R.D., Hyten, D.L., Nelson, R.L., Hartman, G.L. and Diers, B.W. (2009). Mapping and Confirmation of a New Allele at Rpp1 from Soybean PI 594538A Conferring RB Lesion–Type Resistance to Soybean Rust. Crop Science, 49, 783–790. https://doi.org/10.2135/cropsci2008.06.0335 https://doi.org/10.2135/cropsci2008.06.0...
PI 587880A	Rpp1-b	18	Sat_064 (56333703)	Satt191 (54450956)	1882.1	Ray et al. 2009Ray, J.D., Morel, W., Smith, J.R., Frederick, R.D. and Miles, M.R. (2009). Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A. Theoretical Applied Genetic, 119, 271–280. https://doi.org/10.1007/s00122-009-1036-z https://doi.org/10.1007/s00122-009-1036-...
PI 594723	Rpp1*	18	-	-	-	-
PI 230970	Rpp2	16	BARCSOYSSR_ 16_0902 (29253155)	BARCSOYSSR_ 16_0908 (26441233)	188.1	Yu et al. 2015Yu, N., Kim, M., King, Z.R., Harris, D.K., Buck, J.W., Li, Z. and Diers, B.W. (2015). Fine mapping of the Asian soybean rust resistance gene Rpp2 from soybean PI 230970. Theoretical Applied Genetic, 128, 387–396. https://doi.org/10.1007/s00122-014-2438-0 https://doi.org/10.1007/s00122-014-2438-...
PI 506764	Rpp3,	6	satt460 (44049891),	Satt307 (46820834),	2770.9	Monteros et al. 2007;
PI 506764	Rpp5	3	Satt275 (29862641)	Sat_280 (32670690)	2808.1	Kendrick et al. 2011Kendrick, M.D., Harris, D.K., Ha, B.K., Hyten, D.L., Cregan, P.B., Frederick, R.D., Boerma, H.R. and Pedley, K.F. (2011). Identification of a Second Asian Soybean Rust Resistance Gene in Hyuuga Soybean. Phytopathology, 101, 535–543. https://doi.org/10.1094/PHYTO-09-10-0257 https://doi.org/10.1094/PHYTO-09-10-0257...
PI 459025A	Rpp4	18	Satt288 (51127425)	Af162283 (57436765)	6309.4	Silva et al. 2008Silva, D.C.G., Yamanaka, N., Brogin, R.L., Arias, C.A.A., Nepomuceno, A.L., Di Mauro, A.O., Pereira, S.S., Nogueira, L.M., Passianotto, A.L., Abdelnoor, R.V. (2008). Molecular mapping of two loci that confer resistance to Asian rust in soybean. Theoretical Applied Genetic, 117, 57–63. https://doi.org/10.1007/s00122-008-0752-0 https://doi.org/10.1007/s00122-008-0752-...
PI 200487	Rpp5	3	Satt275 (29862641)	Sat_280 (32670690)	2808.1	Lemos et al. 2011

Pop^a a Population, crosses between the soybean rust-susceptible parental and resistant sources, described on Table 1.	Gene	PI source	Chr.^b b Chr., Chromosome.	Position (cM) of LOD peak	Flanking Marker	Marker interval		1.5 interval (cM)	LOD^d d LOD, the logarithm of the odds.	P^e e P, probability of significance, calculated by single-factor analysis of variance.	R² ^f f R2, coefficient of determination calculated based on the nearest marker by regression analysis.	Additive effect^g g Positive additive and dominance effects represent an increase in the value of the trait when the resistant allele (AA) is substituted with the susceptible allele (BB), and negative effects a decrease in the value of the trait.	Dominance effect^g g Positive additive and dominance effects represent an increase in the value of the trait when the resistant allele (AA) is substituted with the susceptible allele (BB), and negative effects a decrease in the value of the trait.
1	Rpp1	PI 200492	18	-c	-	-	-	-	-	-	-	-	-
2	Rpp1-b	PI 594538A	18	11.0	M6	M10	M5	7.3 - 13.8	22.0	<0.001	68.3	1.77	0.40
3	Rpp1-b	PI 587880A	18	3.0	M6	M10	M4	0.0 - 8.2	15.8	<0.001	57.6	1.70	0.54
4	Rpp1*	PI 594723	18	7.0	M5	M6	M1	2.7 - 12.4	9.7	<0.001	42.2	0.80	0.48
5	Rpp1*	PI 594723	18	2.1	M6	M6	M1	2.1 - 9.8	20.9	<0.001	27.8	0.67	0.24
6	Rpp2	PI 230970	16	1.0	M14	M14	M13	0.3 - 3.3	9.1	<0.001	14.1	0.42	0.35
7	Rpp5	PI 506764	3	0.0	M28	M28	M27	0.0 - 8.1	3.1	<0.001	5.3	0.15	-0.06
7	Rpp3		6	13.0	M17	M20	M16	6.8 - 15.9	7.2	<0.001	12.4	0.37	-0.20
8	Rpp4	PI 459025A	18	3.0	M26	M26	M22	3.0 - 29.6	2.2	0.009	3.2	0.29	-0.14
9	Rpp5	PI 200487	3	0.0	M28	M27	M28	0.0 - 4.4	3.6	<0.001	5.8	0.44	0.08
10	Rpp5	PI 200487	3	0.7	M27	M27	M28	0.0 - 0.7	4.1	<0.001	6.4	0.38	0.26

[1] *Corresponding author: benin@utfpr.edu.br

Brasil

Brasil

Rpp genes conferring resistance to Asian soybean rust in F₂ population in the field conditions

ABSTRACT

Brasil

Brasil

Rpp genes conferring resistance to Asian soybean rust in F2 population in the field conditions

ABSTRACT

Rpp genes conferring resistance to Asian soybean rust in F₂ population in the field conditions