Tenecteplase intracameral durante a facoemulsificação em coelhos: avaliação clínica do segmento anterior e análise bioquímica do humor aquoso

Figure 1
The images illustrate the moments of clinical evaluations (corneal opacities, anterior chamber evaluation, and iris evaluation) that were submitted to the animals. TP₀ - preoperative period; TP₁ - first postoperative day; TP₃ - third postoperative day; TP₇ - seventh postoperative day; TP₁₅ - 15th postoperative day and TP₂₁ - 21st postoperative day. The white arrows point to the area of corneal opacity and the stars indicate the area of inflammation of the iris identified by the change of coloration in relation to TP₀

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Graphic 1
Mean of corneal opacity, aqueous flare, anterior chamber fibrin and iris inflammation and representative images of eyes of New Zealand White male rabbits submitted to the phacoemulsification procedure and distributed in UG groups (untreated group) and TG (group treated with tenecteplase 0.1 ml to 50 μg intracameral intraoperatively) at different times of clinical evaluation.

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Table 1
Scores of diffuse corneal opacity, aqueous flare, fibrin and hyphema in the anterior chamber and iris inflammationi⁽¹⁷17 Eaton JS, Miller PE, Bentley E, Thomasy SM, Murphy CJ. Slit Lamp-Based Ocular Scoring Systems in Toxicology and Drug Development: A Literature Survey. J Ocular Pharmacology and Therapeutics. 2017; 10(33).^,¹⁸18 Oréfice F, Filho RC, Barboza AL, Orécife JL, Calucci D. Acquired ocular toxoplasmosis, post-birth ocular toxoplasmosis. Rev Bras Oftalmol, 2010; 69(3): 184-207.^,¹⁹19 Zarei R, Azimi R, Moghimi S, Abdollahi A, Amini H, Eslami Y, Fakhraii G. Inhibition of intraocular fibrin formation after infusion of low-molecular-weight heparin during combined phacoemulsification-trabeculectomy surgery. J Cataract Refract Surg., 2006; 32(11): 1921-1925.⁾

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Table 2
Means and standard errors for IOP of New Zealand rabbits subjected to phacoemulsification and trans operatively treated (TG) or not (UG) with intracameral TNK (p<0.05)

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Table 3
Means and standard errors for aqueous humor pH, density and chloride ion and total protein concentrations among New Zealand rabbits at TP₂₁

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Table 4
Frequency of histological changes in eyeballs of New Zealand White rabbits distributed per treated (TG) and untreated (UG) groups at TP₂₁

Parameters	Score	Description
Corneal opacity	0	Absent
	1	Discrete, good visualization of iris details
	2	Moderate, reduced visualization of iris details
	3	Severe, iris details not visible
Aqueous flare	0	Absent
	1	Discrete, good visualization of iris details
	2	Moderate, reduced visualization of iris details
	3	Severe, iris details not visible
Fibrin	0	Absent
	1	Discrete, affects only 1 quadrant of the pupillary
	2	Moderate, affects 2 to 3 quadrants of the pupillary
	3	Severe, affects the entire pupillary margin
Hyphema	+	Present
Hyphema	-	Absent
Iris inflammation	0	Absent
	1	Discrete, with focal hyperemia, without changes in the
	2	Moderate, with diffuse hyperemia and reduced red
	3	Severe, with diffuse hyperemia and loss of the red
Synechia	+	Present
Synechia	-	Absent

Time-point	IOP(mmHg)		p value
Time-point	UG	TG	p^* * p values between UG and TG.	p^ p values between TP0 and other time-points for UG.	p^* ***** p values between TP0 and other time-points for TG. Students t-test at 5% significance.
TP₀	12.08 (2.26)	11.11 (1.65)	0.13
TP₁	25.77 (13.10)^A	19.32 (12.79)^B	0.20	0.0017	0.0046
TP₃	11.10 (4.13)	10.49 (3.24)	0.64	0.335	0.59
TP₇	9.92 (2.66)^a	9.88 (3.61)	0.97	0.029	0.18
TP₁₅	7.99 (3.61)^A	8.05 (3.41)^B	0.95	0.004	0.0018
TP₂₁	9.27 (3.39)	9.69 (2.83)	0.77	0.0618	1.28

Parameters	Results	CG	UG	TG	P
pH	Mean	8	8.1	8	0.558
pH	Standard error	(0.22)	(0.10)	(0.10)	0.558
Density	Mean	1.008	1.012	1.011	0.0187
Density	Standard error	(0.0004)	(0.001)	(0.0019)	0.0187
Chloride (mEq/ml)	Mean	170.25	151.83	176.78	0.7844
Chloride (mEq/ml)	Standard error	12.33	9.89	19.15	0.7844
Proteins (mg/dl)	Mean	435	998.33	855	0.1955
Proteins (mg/dl)	Standard error	(219.26)	(176.17)	(272.45)	0.1955

Histological changes	TG	UG
Adhesions	16.76%	33.40%
Fibrin	49.91%	83.11%
Fibrosis	24.80%	41.28%
Hemorrhage	66.51%	57.97%
Ciliary adhesions	18.30%	36.36%

[1] *Correspondent - lidiana_piveta@hotmail.com

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Tenecteplase intracameral durante a facoemulsificação em coelhos: avaliação clínica do segmento anterior e análise bioquímica do humor aquoso

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