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Crop Breeding and Applied Biotechnology, Volume: 18, Número: 3, Publicado: 2018
  • Association of candidate genes for fatty acid content in soybean by temperature-switch PCR (TSP) genotyping Article

    Bueno, Rafael Delmond; God, Pedro Ivo Vieira Good; Prata, Isadora Oliveira; Pereira, Pedro Henrique Scarpelli; Teixeira, Arlindo Inês; Piovesan, Newton Deniz; Barros, Everaldo Gonçalves de

    Resumo em Inglês:

    Abstract The development of molecular markers is essential for improvement of soybean cultivars with modified fatty acid content. The objective of this study was to identify and validate SNP markers in candidate genes for fatty acid content in soybean. Six candidate genes (ARAF, PDAT, ABI3, FAD2-1b, FAD3B, and FAD3C) were selected. Alignment of gene sequences identified 25 SNPs and 3 INDELs. TSP primers were used to identify SNP alleles. 259 recombinant inbred lines (RILs) (FA22 / CD219) and 185 F2 progenies (A29 / Tucunaré) were tested for association of SNPs. An SNP for FAD3B was associated with variation in content of linoleic acid (R2 = 5.84%) and linolenic acid (R2 = 6.79%). In FAD3C, an SNP was associated with linoleic and linolenic acids (R2 of 9.21% and 18.51%, respectively). The ABI3 gene was associated with palmitic acid, with R2 = 5.41%. The SNP markers identified will be used in assisted selection for improvement of fatty acid content.
  • Inheritance of harvest index in common bean Article

    Pinto Júnior, Ricardo Andrade; Patto¹, Magno Antonio; Martins¹, Emanoel Sanches; Abreu², Ângela de Fátima Barbosa

    Resumo em Inglês:

    Abstract The aim of this study was to verify if the harvest index (HI) of common bean is higher in modern lines, to verify if its estimate varies with the cycle of the plant and environmental conditions, and to obtain information concerning its genetic control (through diallel crossing). For this purpose, six lines were crossed in a diallel. Evaluations were carried out in three crop seasons/generations - F2, F3, and F4. A receptacle was used to collect leaves, pods, and other plant parts that fell before harvest. Diallel analysis was performed using Griffing's method II. It found that the HI was higher in modern lines and was not affected by the cycle; the estimated HI heterosis was negative, indicating the occurrence of dominance in order to reduce trait expression.
  • Genetic parameters and selection of macaw palm (Acrocomia aculeata) accessions: an alternative crop for biofuels Article

    Costa, Annanda Mendes; Motoike, Sérgio Yoshimitsu; Corrêa, Thais Roseli; Silva, Thaís Cristina; Coser, Sara Morra; Resende, Marcos Deon Vilela de; Teófilo, Reinaldo Francisco

    Resumo em Inglês:

    Abstract This study aimed to estimate the parameters related to the genetic control of the physical characteristics of the fruits, oil content, and oil yield, and to proceed with the selection of macaw palm accessions. Forty-four macaw palm accessions of the active germplasm collection of the Federal University of Viçosa were collected for the evaluation of epicarp dry matter, pulp dry matter, endocarp dry matter, kernel dry matter, oil content, and oil yield. Narrow-sense individual heritability estimates were considered as of intermediate magnitude. The coefficient of repeatability and the accuracy in family selection was of high magnitude. Oil yield per plant presented the highest coefficient of individual genetic variation. Five different accessions contributed to the ten first individuals selected by the individual BLUP. The Mulamba and Mock’s rank index allowed classifying the accessions in a sequence favorable to selection.
  • Potential of soybean crosses in early inbreeding generations for grain yield Article

    Rocha, Gabriela Antônia de Freitas; Pereira, Fernanda Aparecida de Castro; Vello, Natal Antonio

    Resumo em Inglês:

    Abstract The present study aimed to evaluate the combining ability of 16 soybean genotypes using a partial diallel. Two experiments were carried out to estimate the general (GCA) and specific (SCA) combining ability, one with 64 crosses and the other with 16 parents. At the R8 stage, F2 plants and the parents were evaluated for the following traits: number of days to flowering (NDF), number of days to maturity (NDM), plant height at maturity (PHM), and grain yield (GY). Results indicated genetic variability between the parents and between the crosses. For GY, six (37.5%) parents stood out with high yield. The GCA and SCA estimates were significant for all traits, revealing additive and non-additive effects. The parent USP 04-17.027 (4) was superior for presenting the highest means, heritability, and GCA. The cross (4x13 = USP 04-17.027 x USP 231-2224-12) stood out owing to the highest SCA for GY.
  • Combining ability as a strategy for selecting common bean parents and populations resistant to white mold Article

    Ferreira, Lenio Urzeda; Melo, Patrícia Guimarães Santos; Vieira, Rogério Faria; Lobo Junior, Murillo; Pereira, Helton Santos; Melo, Leonardo Cunha; Souza, Thiago Lívio Pessoa Oliveira de

    Resumo em Inglês:

    Abstract Common bean parents and populations resistant to white mold (WM, caused by Sclerotinia sclerotiorum) were selected based on estimates of general combining ability (GCA) and specific combining ability (SCA) for WM severity in three field nurseries in Brazil. Twenty-seven populations were obtained by partial diallel crosses between parents from two groups: GI) three sources of partial resistance identified abroad and GII) nine Brazilian cultivars and elite lines. Populations were advanced in bulk up to the F6 generation. The overall mean of WM severity from combined analysis ranged from 2.83 to 5.03 (scale of 1 to 9 scores) in the F6 population. The score of the susceptible control BRS Requinte was 7.21. The GI parents K-59, in Oratórios, MG, and Viçosa, MG, and K-407 and PI204717, in Goianira, GO, contributed to increase resistance to WM. The most promising populations for obtaining elite lines resistant to WM were K-59/BRS Executivo, PI204717/BRS Campeiro, PI204717/Jalo Precoce, K-59/BRS Radiante, and K-407/BRS Cometa.
  • Genetic parameters and predicted gains with selection of interspecific hybrids of Paspalum for seed production Article

    Lopes, Rodrigo Ramos; Franke, Lucia Brandão; Souza, Cléber Henrique Lopes de; Bertoncelli, Patrícia; Graminho, Larissa Arnhold; Pereira, Émerson André

    Resumo em Inglês:

    Abstract The aim of this study was to estimate the genetic parameters and predicted gains with selection of interspecific hybrids of Paspalum in relation to seed production traits Data were analyzed in randomized block design, with 23 genotypes arranged into ten blocks, according to the methodology of mixed models by the REML/BLUP procedure. The traits considered in the study were: number of total tillers/plant, number of reproductive tillers/plant, number of racemes/inflorescence, number of seeds/inflorescence, weight of a thousand seeds and seed production. Variability among genotypes, high heritability (>0.50), except total tillers (0.44) and number of racemes (0.36), and high accuracy (>0.90) were identified for all traits. The hybrids 10E5052, 10E4026, 10E507, 10E4025 and 10E40104 are among the top ten because they have high genetic values in three or more traits, indicating that these genotypes should be recommended for direct use in planting or potential parents to be used in new crosses.
  • Diversity between Jatropha curcas L. accessions based on oil traits and X-ray digital images analysis from it seeds Article

    Pinto, Milena Silva; Damasceno Junior, Pedro Corrêa; Oliveira, Lucas Couto de; Machado, Adelia Ferreira de Faria; Souza, Marco Andre Alves de; Muniz, Dandara Rêgo; Dias, Luiz Antônio dos Santos

    Resumo em Inglês:

    Abstract Jatropha curcas L. is a species with high potential for the biodiesel production. Therefore, the oil yield and quality from it seeds should be known. This study aimed to estimate the correlation between morphological traits, yield and oil quality evaluated in the seeds, to know the genetic diversity between J. curcas accessions and it behavior regarding to three harvest seasons. For this purpose, X-ray digital images from seeds of 18 accessions were obtained, and the seeds analyzed for external and internal morphological aspects. The same seeds were analyzed for oil yield and quality. In general, the results concerning diversity indicated a narrow genetic base. More rounded and very long seeds had lower oil yield. Larger seeds presented higher oil yield only when their internal cavity was filled by their endosperm. Most of the accessions showed variation regarding the size of the endosperm, yield and oil quality between harvest seasons.
  • Directives and requirements for genetically modified (GM) crop regulation in Argentina Review

    Orroño, Daniela Inés; Vesprini, Facundo

    Resumo em Inglês:

    Abstract Regulation of GM crops prior to their commercial release requires research under controlled conditions, either in confined field trials, in greenhouses, and/or in the laboratory. Authorizations in Argentina can be obtained for experimental or confined field trials and “counter-season” production of regulated seed. These trials represent the first controlled introduction of GM crops in the environment. The goal is to prevent dissemination of GM pollen, seed, or plant parts outside the growing area and avoid consumption by humans and animals. During the trials, data is also collected on agronomic performance and potential environmental impacts. The purpose of this document is to provide a summary of the regulatory requirements governing contained and confined field trials of GM crops in Argentina.
  • SSR-based genetic analysis of sweet corn inbred lines using artificial neural networks Note

    Ferreira, Fernando; Scapim, Carlos Alberto; Maldonado, Carlos; Mora, Freddy

    Resumo em Inglês:

    Abstract Studies on genetic diversity and population structure provide basic information at the molecular level, which is a key input for breeding programs of crop species. This study evaluated the genetic diversity of 12 elite lines of sweet corn, using 20 microsatellite markers. To determine the genetic differentiation among lines, we used an artificial neural network with the self-organizing map (SOM) algorithm. This algorithm identified three genetically differentiated groups and produced relatively more accurate results than UPGMA, according to the indices of Davies-Bouldin and RMSSTD (Root Mean Square Standard Deviation). The expected heterozygosity was high (He>0.5) for 90% and the polymorphism information content high (PIC>0.6) for 40% of the SSR loci, indicating their potential to detect genetic differences among lines. The high genetic differentiation, detected by the neural network procedure, would allow the selection of promising divergent sweet corn genotypes.
  • Research priorities for next-generation breeding of tropical forages in Brazil Note

    Pereira, Jorge Fernando; Azevedo, Ana Luisa Sousa; Pessoa-Filho, Marco; Romanel, Elisson Antonio da Costa; Pereira, Antonio Vander; Vigna, Bianca Baccili Zanotto; Souza Sobrinho, Fausto de; Benites, Flávio Rodrigo Gandolfi; Ledo, Francisco José da Silva; Brito, Giovani Greigh de; Meireles, Karem Guimarães Xavier; Cavallari, Marcelo Mattos; Simeão, Rosângela Maria; Machado, Juarez Campolina

    Resumo em Inglês:

    Abstract Pasture is the main food source for more than 200 million cattle heads in Brazil. Although Brazilian forage breeding programs have successfully released well-adapted, high-yielding cultivars over the years, the use of genomic tools in these programs is currently limited. These tools are required to tackle the main challenges for tropical forage breeding in Brazil. In this context, this notes lists the main research priorities raised at the workshop “Breeding Forages in the Genomic Era”, which are necessary to accelerate the use of genomic tools for next-generation breeding of tropical forages and allow breeders to increase genetic gains. Additionally, an online discussion forum (hosted at http://www.cnpgl.embrapa.br/genfor) has been launched to strengthen collaborations among research groups. The research priorities and more synergistic collaborations will assist researchers and decision-makers in delivering a sustainable increase in production of animal products, especially beef and milk, which are required to feed a rising world population.
  • Estimation of genetic parameters and selection for rooting capacity in peach Note

    Oliveira, João Alison Alves; Bruckner, Cláudio Horst; Silva, Danielle Fabíola Pereira da; Santos, Carlos Eduardo Magalhães dos; Penso, Gener Augusto; Aquino, César Fernandes

    Resumo em Inglês:

    Abstract One of the most important criteria in the improvement of rootstocks is easiness of propagation. The objective of the present study was to verify the existence of genetic variability and the possibility of gains with selection in the rooting capacity among peach genotypes. Seventy-seven genotypes of peach belonging to five F3 families were evaluated. Rooting percentage (RP), number of roots per cutting (NR) and mean root length (RL) were evaluated. Genetic values ​​were estimated. The nineteen genotypes with the lowest rank summation index were selected to estimate the genetic gains. The variables RP, NR and RL had progeny mean heritabilities of 0.53, 0.60 and 0.83, respectively. Of the individuals selected, 89.5% belonged to the 713 (Talismã x Adafuel) family. The estimation of the genetic and phenotypic parameters show the possibility of success by selecting superior genotypes, with gains of 9.09, 17.47 and 24% for RP, NR and RL, respectively.
  • RB036066 - a sugarcane cultivar with high adaptability and yield stability to Brazilian South-Central region Cultivar Release

    Daros, Edelclaiton; Oliveira, Ricardo Augusto de; Zambon, José Luis Camargo; Bespalhok Filho, João Carlos; Brasileiro, Bruno Portela; Ido, Oswaldo Teruyo; Ruaro, Lucimeris; Weber, Heroldo

    Resumo em Inglês:

    Abstract The sugarcane cultivar RB036066 is medium-maturing and has a long period of industrial suitability; in the South-Central region, harvest is recommended between June and September, and it is indicated for planting on medium to highly fertile soils. The cultivar is widely adaptable and has high sugar yield and stability of agricultural production.
  • IAC Obatã 4739 - dwarf arabic coffee cultivar with yellow fruits and resistant to leaf rust Cultivar Release

    Fazuoli, Luiz Carlos; Braghini, Masako Toma; Silvarolla, Maria Bernadete; Gonçalves, Wallace; Mistro, Júlio César; Gallo, Paulo Boller; Guerreiro Filho, Oliveiro

    Resumo em Inglês:

    Abstract Cultivar IAC Obatã 4739 was derived from a cross between coffee tree IAC 1669-20-1 and cultivar Catuaí Amarelo IAC 62. It is short and high-yielding, rust-resistant, has large and yellow fruits, medium to late maturation, excellent cup quality, and responds effectively to irrigation.
  • CD 1705 - healthy and high-yielding wheat cultivar for biscuit manufacturing Cultivar Release

    Franco, Francisco de Assis; Marchioro, Volmir Sergio; Evangelista, Adriel; Polo, Mateus; Montecelli, Tatiane Dalla Nora; Souza, Leandro Vagno; Schuster, Ivan; Lima, Fábio Junior Alcântara de; Santos, Diego Augusto dos; Grave, Edson Luiz

    Resumo em Inglês:

    Abstract CD 1705 is a cultivar indicated for the wheat-producing regions 1, 2 and 3 of the states of Rio Grande do Sul, Santa Catarina and Paraná. It is appropriate for biscuit manufacturing, has good plant health and high production potential (mean yield of 4308 kg ha-1).
  • IAC Sintonia: new carioca common bean cultivar Cultivar Release

    Chiorato, Alisson Fernando; Carbonell, Sérgio Augusto Morais; Gonçalves, João Guilherme Ribeiro; Silva, Daiana Alves da; Benchimol-Reis, Luciana Lasry; Carvalho, Cassia Regina Limonta; Barros, Vera Lúcia Nishijima Paes de; Freitas, Rogério Soares de; Ticelli, Marcelo; Gallo, Paulo Boller; Santos, Neli Cristina Belmiro dos

    Resumo em Inglês:

    Abstract IAC Sintonia, new common bean cultivar, was developed to incorporate moderate resistance to the main pathogens Fusarium oxysporum and Colletotrichum lindemuthianum allied to great grain yield observed, 5,750.00 kg ha-1. IAC Sintonia has semi-upright architecture and indeterminate growth habit (Type II), cycle is around 90 days and, 1000 seed weight of 280 grams.
  • IPRDataCol - software for field labelling and data storage Software Release

    Garbuglio, Deoclecio Domingos

    Resumo em Inglês:

    Abstract The software IPRDataCol was developed in 2017 at the Instituto Agronômico do Paraná (IAPAR) by a research group (Nucleo1) studying information technology applied to agricultural research. Due to the software architecture, whether the label-generating nor the data capture module use any native database, i.e, the program can be used directly from portable storage devices with flash memory. It is designed as a support tool to meet the needs of different research projects, regarding optimization in the identification of experimental plots in the field or laboratory/greenhouse, with subsequent logging of yield data measured by electronic scales, and storage in digital files. The software can be downloaded free of charge from the internet (http://nucleo1.org), without registration. The use of this program will contribute to minimize errors in data acquisition since the typing phase is not necessary for yield data measured by electronic scales.
Crop Breeding and Applied Biotechnology Universidade Federal de Viçosa, Departamento de Fitotecnia, 36570-000 Viçosa - Minas Gerais/Brasil, Tel.: (55 31)3899-2611, Fax: (55 31)3899-2611 - Viçosa - MG - Brazil
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