Genetic characterization of remaining populations of <i>paratecoma peroba</i>, an endangered and endemic species of the atlantic forest

França, Tábatta Caroline Cerri; Tavares, Leticia Rigo; Silva Júnior, Adelson Lemes da; Miranda, Fábio Demolinari de; Vargas, Lucas Batista; Abreu, Karla Maria Pedra de; Caldeira, Marcos Vinicius Winckler

doi:10.1590/01047760202228013055

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Ecology • CERNE 28 • 2022 • https://doi.org/10.1590/01047760202228013055 copy

Genetic characterization of remaining populations of paratecoma peroba, an endangered and endemic species of the atlantic forest

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Background:

The objective of this study was the genetic characterization of remaining populations of the species Paratecoma peroba occurring in fragments of the Atlantic Forest, by estimating parameters of diversity and genetic structure. The study was carried out in two forest fragments, the Atlantic Forest Environmental Education Pole (area 1) and the Pacotuba National Forest (area 2), where 93 adult trees were identified.

Results:

Ten Inter Simple Sequence Repeats (ISSR) primers were used in genotyping, obtaining 112 amplified bands with 87.5% polymorphism. The genetic diversity estimated for the populations from the Nei (H*) and Shannon (I*) indices was higher for area 1. For the joint data, moderate genetic diversity was observed, referring to the average values of 0.26 and 0.40, obtained for the H* and I* indices, respectively. Molecular variance analysis resulted in moderate differentiation between populations (Φ _ST = 0.143), while gene flow analysis (N _m = 6.69) revealed the occurrence of similar alleles between them. However, the predominance of a single genetic group in area 2, revealed from Bayesian approach, indicates that its genetic structure, possibly generated by the current scenario of fragmentation of the Atlantic Forest and the distancing of populations, affecting the contemporary gene flow.

Conclusion:

Despite the moderate genetic diversity of the species for the area 2 population, actions toward inclusion of seedlings obtained from seeds from neighboring fragments, including area 1, and the increase in the connectivity of forest fragments through ecological corridors, could assist in augmenting its genetic variation.

Keywords:
Forest conservation; Genetic diversity; ISSR; Peroba do campo; Population structure

HIGHLIGHTS

ISSR markers showed sufficient number of fragments and informative.

Moderate genetic diversity was found for P. peroba.

The area 1 population is an important source of propagules for the conservation of the species.

The area 2 population is in the process of genetic structuring.

Primers	Primer sequence (5’-3’)*	NTB	NPB	PPB (%)	PM _Area1	PM _Area2	SVF (máx-mín)
UBC 807	AGA GAG AGA GAG AGA GT	14	13	92.85	4	0	980 – 200
UBC 810	GAG AGA GAG AGA GAG AT	5	4	80.00	1	1	2080 – 400
UBC 811	GAG AGA GAG AGA GAG AC	9	7	77.77	0	0	2080 – 400
UBC 812	GAG AGA GAG AGA GAG AA	13	12	92.30	2	0	2080 – 310
UBC 815	CTC TCT CTC TCT CTC TG	8	8	100.00	0	1	1600 – 550
UBC 827	ACA CAC ACA CAC ACA CG	12	9	75.00	0	1	2080 – 480
UBC 836	AGA GAG AGA GAG AGA GYA	14	12	85.71	0	1	1200 – 320
UBC 845	CTC TCT CTC TCT CTC TTT	15	13	86.66	0	0	2080 – 320
UBC 868	GAA GAA GAA GAA GAA GAA	12	11	91.66	0	0	1080 – 480
UBC 880	GGA GAG GAG AGG AGA	10	9	90.00	5	0	1700 – 300
Total	-	112	98	87.50	12	4	-

*Loci* (UBC’s)	Area 1					Area 2					Joint analysis
*Loci* (UBC’s)	PIC	A _O	A _E	H*	I*	PIC	A _O	A _E	H*	I*	PIC	A _O	A _E	H*	I*
807	0.26	1.92	1.39	0.25	0.40	0.25	1.64	1.47	0.25	0.36	0.29	1.92	1.45	0.29	0.45
810	0.10	1.60	1.45	0.25	0.37	0.21	1.60	1.40	0.20	0.30	0.27	1.80	1.44	0.27	0.41
811	0.20	1.77	1.31	0.20	0.32	0.29	1.77	1.50	0.29	0.42	0.25	1.77	1.42	0.25	0.39
812	0.29	1.76	1.45	0.29	0.45	0.11	1.38	1.17	0.09	0.15	0.25	1.92	1.36	0.24	0.39
815	0.26	2.00	1.39	0.26	0.43	0.22	1.75	1.36	0.22	0.33	0.26	2.00	1.39	0.26	0.41
827	0.23	1.75	1.34	0.22	0.34	0.28	1.75	1.50	0.28	0.42	0.26	1.83	1.40	0.26	0.41
836	0.28	1.85	1.49	0.28	0.41	0.30	1.85	1.53	0.30	0.45	0.29	1.85	1.52	0.29	0.43
845	0.23	1.86	1.36	0.22	0.35	0.25	1.80	1.44	0.25	0.38	0.25	1.93	1.40	0.25	0.39
868	0.25	1.83	1.40	0.25	0.38	0.24	1.91	1.40	0.24	0.37	0.25	1.91	1.40	0.25	0.40
880	0.25	1.90	1.40	0.24	0.38	0.24	1.90	1.38	0.23	0.37	0.25	1.90	1.40	0.25	0.39
Average	0.23	1.82	1.39	0.24	0.38	0.24	1.73	1.41	0.23	0.35	0.26	1.88	1.41	0.26	0.40

Source of variation	Degrees of freedom	Sum of squares	Variance components	Variation (%)
Between populations	1	109.26	2.30	14.30
Within populations	91	1259.25	13.83	85.70
Total	92	1368.51	16.14	-
Φ_ST = 0.143

UFLA - Universidade Federal de Lavras Universidade Federal de Lavras - Departamento de Ciências Florestais - Cx. P. 3037, 37200-000 Lavras - MG Brasil, Tel.: (55 35) 3829-1706, Fax: (55 35) 3829-1411 - Lavras - MG - Brazil
E-mail: cerne@dcf.ufla.br

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[1] ✤Corresponding author e-mail tabattacerri17@gmail.com